Functional analysis of dwarf genes located in T-DNA of Ri plasmids and its application to plant breeding
Functional analysis of dwarf genes located in T-DNA of Ri plasmids and its application to plant breeding
批准号:
63490005
负责人:
KAMADA Hiroshi
金额:
$2.75万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (B)
财政年份:
1988
资助国家:
日本
项目状态:
已结题
起止时间:
1988 至 1989
中文摘要
菊苣花芽分化需要低温长日照处理。然而,用Ri质粒的T-DNA转化的菊苣植株在长日照条件下不经低温处理就表现出开花。在转Ri质粒T-DNA上的rol C基因的菊苣植株中也观察到类似的不经低温处理的花芽形成现象。用赤霉素、反赤霉素、细胞分裂素和乙烯等植物生长调节剂处理未转化的菊苣植株,赤霉素(GA_3)诱导茎伸长,但不诱导花芽分化。抗赤霉素(S-3307,一种有效的赤霉素生物合成抑制剂)对菊苣花芽分化的诱导频率较低,而且当用GA_3处理转Ri T-DNA的菊苣植株时,不能观察到花芽的形成。这些结果表明,Ri T-DNA可能具有抑制赤霉素生物合成或抑制赤霉素活性的生理功能。转化植株和正常植株的赤霉素主要为GA_1,转化植株中GA_1的含量低于正常植株的1/100。这一结果表明,一些生理反应(包括矮化),特别是在植物中观察到的Ri T-DNA转化诱导内源赤霉素含量的减少转化植物。
英文摘要
It is well known that Cichorium intybus requires low temperature and long day treatments for the flower bub differentiation. However, Cichorium plants transformed with T-DNA of Ri plasmid exhibited flower formation without low temperature treatment under a long day condition. Similar phenomenon of flower bud formation without low temperature treatment was also observed in Cichorium plants transformed with single rol C gene located on the T-DNA of Ri plasmid. When not-transformed (normal) Cichorium plants were treated with some plant growth regulators, such as gibberellin, anti-gibberellin, cytokinin, and ethylene, gibberellin (GA_3) induced the stem elongation, but not the flower bud differentiation. On the other hand, anti-gibberellin (S-3307, a potent inhibitor of gibberellin biosynthesis) induced the flower bud differentiation with low frequency.Moreover, when Cichorium plants transformed with Ri T-DNA were treated with GA_3, flower bud formation could not be observed. These results indicate that a possible physiological function of Ri T-DNA is an inhibition of gibberellin biosynthesis or a suppression of gibberellin activity).Qualitative and quantitative analyses of endogenous gibberellin in Atropa belladonna transformed with Ri T-DNA were carried out by using radio-immunoassay with monoclonal antibodies against some gibberellins. The major gibberellin in the transformed and normal plants was GA_1, and the contents of GA_1 in the transformed plants was less than 1/100 as compared to that of the normal plants. This result suggests that some physiological responses (including dwarfism) observed specifically in plants transformed with Ri T-DNA were induced by the decrease of endosenous gibberellin contents in the transformed plants.
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鎌田博、小野明美: 組織培養. (1989)
Hiroshi Kamata,Akemi Ono:组织培养(1989)。
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R.Kato: "Effects of high and very magnetic fields on the growth of hairy roots of Daucus carota and Atropa belladonna." Plant Cell Physiol.30. 605-608 (1989)
R.Kato:“高磁场和强磁场对胡萝卜和颠茄毛状根生长的影响。”
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H.Ohkawa: "Effects of gibberellic acid on hairy root growth in Datura innoxia." J.Plant Physiol.134. 633-636 (1989)
H.Ohkawa:“赤霉酸对曼陀罗毛状根生长的影响。”
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A.Isogai;N.Fukuchi;M.Hayashi;H.Kamada;H.Harada;A.Suzuki: Agric Biel Chem. 52(12). 3235-3237 (1988)
A.Isogai;N.Fukuchi;M.Hayashi;H.Kamada;H.Harada;A.Suzuki:Agric Biel Chem。
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H.Kamada: "Glbberellin Symposium Tokyo 1989(Effenoxia.cts of gibberellic acid on growth and tropane alkaloid synthesis in Ri transformed plant of Datura innoxia.(印刷中)" Springer-Verlag(eds.by N.Takahashi,B.O.Phinney and J.MacMillan),
H. Kamada:“Glbberellin Symposium Tokyo 1989(Effenoxia.cts of赤霉酸对 Ri 转化植物 of Datura innoxia 的生长和托烷生物碱合成。(印刷中)”Springer-Verlag(由 N.Takahashi、B.O.Phinney 和 J 编辑) .麦克米伦),
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共 23 条
Effect of Screening and Prevention Programs for Musculoskeletal Problems in Elementary and Junior High School Students
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负责人:KAMADA Hiroshi
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Japan-France Joint Study on Development in Higher Plants
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依托单位:
Analysis of flower induction by Ri rolC gene and its application to plant breeding
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项目类别:Grant-in-Aid for General Scientific Research (B)
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资助金额:$3.52万
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财政年份:1992
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负责人:KAMADA Hiroshi
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依托单位:
海外基金