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Detection of Oncogenes Affecting Genetic Diagnosis and Prognosis in the Patients with Oral Cancer by CGH

CGH检测影响口腔癌基因诊断及预后的癌基因

基本信息

批准号：
07407056
负责人：
AMAGASA Teruo
金额：
$ 12.86万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07407056/
关键词：
Comparative Genomic Hybridization(CGH)Oral (Squamous Cell) Carcinoma Chromosome DNA Copy Number Tumor Malignancy Lymph Node Metastasis Comparative Genomic Hybridization (CGH)Comparative Genomic Hybridization (CGH)

项目摘要

1. To find and map chromosomal regions with amplifications and deletions in oral cancsers by CGH,we performed CGH on 17 oral squamous cell carcinomas cell lines and compared to those obtained by Southern hybridization. Recurrent loci for amplification were observed on 8q22-26,3q25-27,7p12,11q13,13q33,14q, 15q and 20q12. Deletions were seen on 3p, 8p, 5q21-22,7q31 and 18q21. There was a strong concordance for amplification detected by the CGH and Southern hybridization methods.2. When the original tumors and the cell lines were compared, their profiles were essestialy similar with one exception. Further, there was no region that commonly changed in the cell lines, but not in the original tumors, suggesting that the DNA copy number changes observed in the cell lines mostly represent those of the original tumors.3. We correlated clinical and histopathological results to tomor DNA copy number changes. The increases on chromosomes 6p23-25 and 8q24 were specificaly observed in the groups of WHO Grade II and Grade III,whereas the deletions on chromosomes 11p11-13 and 18q21 observed in the same groups. Comparison of DNA copy number changes to TNM classification indicated that the deletions on chromosomes 1p36 and 10q25-26 might be related to tumor progression.

1.为了通过CGH寻找并绘制口腔癌中存在扩增和缺失的染色体区域，我们对17个口腔鳞状细胞癌细胞系进行了CGH，并与Southern杂交获得的结果进行比较。在 8q22-26、3q25-27、7p12、11q13、13q33、14q、15q 和 20q12 上观察到扩增的复发位点。在 3p、8p、5q21-22、7q31 和 18q21 上发现缺失。 CGH和Southern杂交检测的扩增结果具有很强的一致性。 2．当对原始肿瘤和细胞系进行比较时，除了一处例外，它们的特征基本上相似。此外，在细胞系中没有常见变化的区域，但在原始肿瘤中却没有，这表明在细胞系中观察到的DNA拷贝数变化大部分代表了原始肿瘤的DNA拷贝数变化。3．我们将临床和组织病理学结果与肿瘤 DNA 拷贝数变化相关联。 6p23-25和8q24染色体的增加在WHO II级和III级组中观察到，而11p11-13和18q21染色体上的缺失在同一组中观察到。 DNA拷贝数变化与TNM分类的比较表明，染色体1p36和10q25-26上的缺失可能与肿瘤进展有关。