Detection of Oncogenes Affecting Genetic Diagnosis and Prognosis in the Patients with Oral Cancer by CGH

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1. To find and map chromosomal regions with amplifications and deletions in oral cancsers by CGH,we performed CGH on 17 oral squamous cell carcinomas cell lines and compared to those obtained by Southern hybridization. Recurrent loci for amplification were observed on 8q22-26,3q25-27,7p12,11q13,13q33,14q, 15q and 20q12. Deletions were seen on 3p, 8p, 5q21-22,7q31 and 18q21. There was a strong concordance for amplification detected by the CGH and Southern hybridization methods.2. When the original tumors and the cell lines were compared, their profiles were essestialy similar with one exception. Further, there was no region that commonly changed in the cell lines, but not in the original tumors, suggesting that the DNA copy number changes observed in the cell lines mostly represent those of the original tumors.3. We correlated clinical and histopathological results to tomor DNA copy number changes. The increases on chromosomes 6p23-25 and 8q24 were specificaly observed in the groups of WHO Grade II and Grade III,whereas the deletions on chromosomes 11p11-13 and 18q21 observed in the same groups. Comparison of DNA copy number changes to TNM classification indicated that the deletions on chromosomes 1p36 and 10q25-26 might be related to tumor progression.