Fundamental study on gene-hyperthermia for cancer using heat sensitive gene

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Hyperthermia for cancer using magnetite particles as heating material has been studied. In the present study, hyperthermia was not performed only by heating alone but also by combination with gene expression and drug incorporation.In order to increase the amount of magnetite adsorbed to a cancer cell, TMAG which is a lipid with positive charge was incorporated to a magnetoliposome. Polyethylene glycol coated magnetite particles were prepared and those amount it tumor tissue was observed by means of Magnetic Resonance Imaging after administrated through a tail vein. It was found that those particles can be used as a magnetic resonance contrast agent. Interferon beta gene was arranged downstream heat inducible promoter and the liposome with the gene was prepared. When the gene-encapsulated liposome was incorporated to glioma cells, the transient expression of interferon-beta was obtained. In order to enhance the killing effect by hyperthermia, the magnetoliposome prepared by us was directly administrated to the tumor tissue, which formed under the skin of a rat. Glioma tissue was observed histologically after 40 min treatment by high efficiency magnetic field with 386 Oe. As a result, it was found that almost all rat glioma cells were dead after 3 times treatment.