Peptideinformatics-establishment of newly screening and design method of functional peptide-
肽信息学-功能肽筛选设计新方法的建立-
基本信息
- 批准号:17206082
- 负责人:
- 金额:$ 31.95万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (A)
- 财政年份:2005
- 资助国家:日本
- 起止时间:2005 至 2007
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Peptides have attracted attention for their ability to regulate a variety of cellular events by interacting with receptors on the cell surface. A large number of biomaterials that conjugate functional peptides have been developed for medicine, drug delivery, tissue engineering, bio-imaging, and food additives. However, identifying functional peptides by exhaustive screening that covers all the possible combinations of 20 amino acids is terribly time-consuming.In this research, “Peptideinformatics" was proposed, which is combined new peptide screening method combined with computational analysis. The method is based on the concept that screening efficiency can be enhanced from even limited data by use of a model derived from computational analysis that serves as a guide to screening and combining it with subsequent repeated experiments. Here we focus on cell adhesion peptides as a model application of this peptide-screening strategy. Cell adhesion peptides were screened by use of a cell-based assay of a peptide array. Starting with the screening data obtained from a limited, random 5-mer library (643 sequences), a rule regarding structural characteristics of cell adhesion peptides was extracted by fuzzy neural network (FMN) analysis. According to this rule, peptides with nnfavored residues in certain positions that led to inefficient binding were eliminated from the random sequences. In the restricted, second random library (273 sequences), the yield of cell adhesion peptides having an adhesion rate more than 1.5-fold to that of the basal array-support was significantly high (31%) compared to the unrestricted random library (20%). In the restricted third library (50 sequences), the yield of cell adhesion peptides increased to 84%. We conclude that a repeated cycle of experiments screening limited numbers of peptides can be assisted by the rule-extracting feature of FNN.
多肽因其通过与细胞表面受体相互作用来调节多种细胞事件的能力而受到关注。大量结合功能肽的生物材料已被开发用于医药、药物输送、组织工程、生物成像和食品添加剂等领域。然而,通过详尽的筛选来识别功能肽,包括20种氨基酸的所有可能组合,是非常耗时的。本研究提出了“肽信息学”,将新的肽筛选方法与计算分析相结合。该方法基于这样一个概念,即即使有限的数据也可以通过使用计算分析得出的模型来提高筛选效率,该模型可以作为筛选的指导,并将其与随后的重复实验相结合。在这里,我们专注于细胞粘附肽作为这种肽筛选策略的模型应用。细胞粘附肽通过使用基于细胞的多肽阵列检测来筛选。从有限的随机5-mer文库(643个序列)中获得筛选数据开始,通过模糊神经网络(FMN)分析提取细胞粘附肽的结构特征规律。根据这一规则,在某些位置上具有导致低效结合的不利残基的肽从随机序列中消除。在受限的第二个随机文库(273个序列)中,与不受限制的随机文库(20%)相比,黏附率超过基础阵列支持的1.5倍的细胞粘附肽的产量(31%)显着高。在限制性第三文库(50个序列)中,细胞粘附肽的产率提高到84%。我们得出的结论是,FNN的规则提取特性可以帮助筛选有限数量的肽的重复实验周期。
项目成果
期刊论文数量(88)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Pentamer peptide from Fas antigen ligand inhibits tumor-growth with solid-bound form found by peptide array
肽阵列发现来自 Fas 抗原配体的五聚体肽以固体结合形式抑制肿瘤生长
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Ryuji Kato;Yukako Okuno;Chiaki Kaga;Mitoshi Kunimatsu;Takeshi Kobayashi;Hiroyuki Honda
- 通讯作者:Hiroyuki Honda
情報処理を用いたペプチドスクリーニングの効率化
利用信息处理提高肽筛选效率
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:加藤竜司;加賀 千晶;国松 己歳;大河内 美奈;本多 裕之
- 通讯作者:本多 裕之
Screening of apoptosis inducible short peptides from trail-derived sequence
从试验衍生序列中筛选凋亡诱导短肽
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Mina Okochi;Chiaki Kaga;MariNakanishi;Ryuji Kato;Hiroyuki Honda
- 通讯作者:Hiroyuki Honda
細胞接着ペプチドによる形態変化と間葉系幹細胞の分化誘導
细胞粘附肽对间充质干细胞的形态变化和分化诱导
- DOI:
- 发表时间:2008
- 期刊:
- 影响因子:0
- 作者:大河内美奈;野村茂幸;加賀千晶;加藤竜司;紀ノ岡正博;本多裕之
- 通讯作者:本多裕之
ペプチドアレイを利用したZnO結合ペプチドの解析
使用肽阵列分析 ZnO 结合肽
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:大河内美奈;古澤聖司;杉田智哉;梅津光央;阿尻雅文;本多裕之
- 通讯作者:本多裕之
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HONDA Hiroyuki其他文献
Detection of QT prolongation through approximation of the T wave on Gaussian mixture modeling
通过高斯混合模型上 T 波的近似来检测 QT 延长
- DOI:
- 发表时间:
2013 - 期刊:
- 影响因子:0
- 作者:
HIBINO Shin;UEDA Norihiro;HORIBA Mitsuru;YASUI Kenji;KAGAMIHARA Yusuke;FUNAHASHI Shuji;KAMIYA Kaichiro;HONDA Hiroyuki - 通讯作者:
HONDA Hiroyuki
HONDA Hiroyuki的其他文献
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{{ truncateString('HONDA Hiroyuki', 18)}}的其他基金
Study of documents distributed at school for foreign parents
研究在学校分发给外国家长的文件
- 批准号:
16K13241 - 财政年份:2016
- 资助金额:
$ 31.95万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Effects and neuroprotection of the anesthetic agent in the spinal ventral horn neuron
麻醉剂对脊髓腹角神经元的影响及神经保护
- 批准号:
26462331 - 财政年份:2014
- 资助金额:
$ 31.95万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Electrophysiological analysis of anesthetic neuroprotective effect
麻醉神经保护作用的电生理分析
- 批准号:
24791582 - 财政年份:2012
- 资助金额:
$ 31.95万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
The physical distribution and community of the 16th century western part of Japan
16世纪日本西部地区的地理分布和社区
- 批准号:
23520814 - 财政年份:2011
- 资助金额:
$ 31.95万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Research on the effects "patriotic education" in the East Asian region has been given to the Japanese-language education
东亚地区“爱国主义教育”对日语教育的影响研究
- 批准号:
21520548 - 财政年份:2009
- 资助金额:
$ 31.95万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Electrophysiological analysis of opioidergic neuroprotective effect in spinal cord ischemia
阿片类药物对脊髓缺血神经保护作用的电生理分析
- 批准号:
21791438 - 财政年份:2009
- 资助金额:
$ 31.95万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Research on single cell function analysis by magnetic force-based cell patterning
基于磁力的细胞图案化单细胞功能分析研究
- 批准号:
21360400 - 财政年份:2009
- 资助金额:
$ 31.95万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Structural Change of Social Economy and Public Power in the Transitional 16th and 17th centuries
16、17世纪转型期社会经济与公共权力的结构变迁
- 批准号:
17520444 - 财政年份:2005
- 资助金额:
$ 31.95万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Design of cancer immunostimulant peptide by designable proteomix
通过可设计蛋白质组设计癌症免疫刺激肽
- 批准号:
15360439 - 财政年份:2003
- 资助金额:
$ 31.95万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of gene expression for apotosis using DNA chips
使用 DNA 芯片分析细胞凋亡的基因表达
- 批准号:
13450341 - 财政年份:2001
- 资助金额:
$ 31.95万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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