Specific Imaging and Intracellular Hyperthermia for Brain Tumor using Magnetite Particle conjugated with monoclonal antibody

使用与单克隆抗体结合的磁铁矿颗粒对脑肿瘤进行特异性成像和细胞内热疗

• 批准号: 08671574
• 负责人: WAKABAYASHI Toshihiko
• 金额: $ 1.47万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08671574/
• 关键词: Magnetite; Glioma; Imaging; Hyperthermia; Liposome; MRI; 造影剤; グリオーマ

In this study, it was demonstrated that magnetite particle coupled to G-22 monoclonal antibody, could be useful for brain tumor imaging on MRI.After injection of this magnetite, tumor bearing mice were exposed to the magnetic field of an MRI scanner and T2 WI intensity in the tumor was compared with reference number. Result indicated a significant decreace of T2 WI intensity ratio in the tumor, suggesting specific accumulation of magnetite in the tumor. To the next study, we have investigated the possibility of hyperthermia for the tumor using this taregt directed magnetite in the high frequent magnetic field. When 375 Oe of magnetic field and 120kHz of frequency were applied, the magnetite in the tumor tissue were stimulated to be enough for heating tumor tissue to induce necrosis. These result suggest that target directed magnetite is expected to be not only for tumor enhancement agent in MRI, but also for the treatment strategy by hyperthermia for the tumor in the high frequent magnetic field.

本研究证实了磁铁矿颗粒与G-22单抗偶联，可用于脑肿瘤的磁共振成像。注射该磁铁矿后，荷瘤小鼠暴露于MRI扫描仪的磁场中，并将肿瘤内的T2加权信号与参考值进行比较。结果显示，肿瘤的T2加权像信号比明显降低，提示肿瘤内有特异性的磁铁矿聚集。在接下来的研究中，我们研究了在高频磁场中使用这种尾部定向磁铁矿进行肿瘤热疗的可能性。当施加375Oe的磁场和120 kHz的频率时，肿瘤组织中的磁铁矿被激发到足以加热肿瘤组织引起坏死。这些结果表明，靶向磁铁矿不仅有望在磁共振成像中作为肿瘤增强剂，而且可以作为高频磁场中肿瘤的热疗策略。

项目成果

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Yanase M. et al.: "Antitemor lmmity Induction by Intracllular Hyperthermin using Nagnetite Cationic Liposomes" Jpn.J.Cancer Res.89. 775-782 (1998)

Yanase M. 等人：“使用 Nagnetite 阳离子脂质体通过细胞内 Hyperthermin 诱导抗炎免疫”Jpn.J.Cancer Res.89。

Masashige Shinkai, et al: "Development of novel magnetite sensing for brain lision using functional magnetic particles" KAGAKUKOUGAKU RONNBUNNSHI. Vol 24(2). 174-178 (1998)

Masashige Shinkai 等人：“利用功能性磁性粒子开发用于脑损伤的新型磁铁矿传感”KAGAKUKOUGAKU RONBUNNSHI。

Yanase M, et al.: "Intracellular Hyperthermia for Cancrusing Magnetite Cationic Liposomes.Exvivostudy" Jpn.J.Caneer Res.88. 630-632 (1997)

Yanase M 等人：“用于癌症磁铁矿阳离子脂质体的细胞内热疗。Exvivostudy”Jpn.J.Caneer Res.88。

Suzuki H,et al: "Development of a target-directed magnetic resonance contrast agent using monulonal antibody-conjugated magnetic partiles" Brain Tumor Pathol. 13. 127-132 (1996)

Suzuki H 等人：“使用单克隆抗体缀合的磁性粒子开发靶向磁共振造影剂”脑肿瘤病理。