Physiological Roles of a Novel Calcium Phospholipid Binding Protein, Annexin V,in the Function of the Anterior Pituitary Gland and the Ovary ; A Molecular-Cellular Biological Approach
新型磷脂钙结合蛋白膜联蛋白 V 在垂体前叶腺和卵巢功能中的生理作用;
基本信息
- 批准号:07456136
- 负责人:
- 金额:$ 4.86万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Physiological importance of a novel calcium-phospholipid binding protein, annexin V,in functions of the anterior pituitary gland and the ovary was investigated. By immunocytochemical approach, the specific distribution of annexin V on gonadotrophs at the anterior pituitary gland was demonstrated. The amount of this protein in the anterior pituitary gland was revealed to increase after ovariectomy. It was shown that ovariectomy enhances annexin V synthesis also by Northern blot analysis. These results led us to examine the effect of GnRH on annexin V synthesis and we found a stimulating effect of GnRH on annexin V mRNA accumulation. In turn, annexin V increased LH beta mRNA.Getting together, annexin V is thought to be a novel signal transducer of GnRH at pituitary gonadotrophs. Annexin V is hypothesized to be a physiological modulator also at the ovary. The distribution of annexin V was shown to restricted to luteal cells. Granulosa cells showed neither annexin V nor its mRNA.Furthermore, luteal annexin V varies its concentration along with pseudopregnancy. These results suggest annexin V would play a role in luteal progestational function. We also examined the effect of annexin V in neural system by observing single unit activity (SUA) recorded with a section of hypothalamus in vitro. Even though we have only preliminary data, annexin V was shown to inhibit or stimulate significantly the neural firing activity. The present project on the physiological role of annexin V raises a possibility that annexin V exerts specific activity locally in various tissues. Among these, its signal transducing action at gonadotrophs is the first observation on its activity of physiological modulator.
研究了一种新的钙磷脂结合蛋白膜联蛋白V在垂体前叶和卵巢功能中的生理重要性。免疫细胞化学方法证实了膜联蛋白V在垂体前叶促性腺上的特异性分布。卵巢切除术后发现垂体前叶中该蛋白的含量增加。Northern blot分析表明,卵巢切除术也能促进膜联蛋白V的合成。这些结果使我们研究了GnRH对膜联蛋白V合成的影响,我们发现GnRH对膜联蛋白V mRNA积累有刺激作用。反过来,膜联蛋白V增加LH β mRNA。综上所述,膜联蛋白V被认为是垂体促性腺激素中GnRH的一种新的信号传感器。膜联蛋白V也被认为是卵巢的生理调节剂。膜联蛋白V的分布仅限于黄体细胞。颗粒细胞未显示膜联蛋白V及其mRNA。此外,黄体膜联蛋白V的浓度随假妊娠而变化。提示膜联蛋白V可能在黄体孕产功能中起一定作用。我们还通过观察下丘脑体外切片记录的单单位活性(SUA)来检测膜联蛋白V在神经系统中的作用。尽管我们只有初步的数据,但膜联蛋白V被证明能显著抑制或刺激神经放电活动。目前关于膜联蛋白V生理作用的项目提出了膜联蛋白V在各种组织中局部发挥特定活性的可能性。其中,其对促性腺激素的信号转导作用是首次观察到其生理调节剂的活性。
项目成果
期刊论文数量(126)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Hirasawa M: "The rostral ventrolateral medulla mediates suppression of the circulatory system by the ventromedial nucleus of the hypothalamus.(in press)" Brain Res. (1996)
Hirasawa M:“延髓头侧腹外侧介导下丘脑腹内侧核对循环系统的抑制。(正在印刷中)”Brain Res。
- DOI:
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- 影响因子:0
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- 通讯作者:
Yoshida S: "Molecular cloning of cDNA for equine ovarian inhibin/activin bA-subunit." J Vet Med Sci. 57. 469-473 (1995)
Yoshida S:“马卵巢抑制素/激活素 bA 亚基 cDNA 的分子克隆。”
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- 影响因子:0
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Hasegawa Y: "Inhibin and Activin;Novel Regulators for Gonadal Function and Sexual Maturation." Ares-Serono Symposia Publications SEXUAL DIFFERENTIATION AND MATURATION(in press), (1996)
长谷川 Y:“抑制素和激活素;性腺功能和性成熟的新型调节剂。”
- DOI:
- 发表时间:
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- 影响因子:0
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- 通讯作者:
Nishimura F: "Excitability of neurons in the ventromedial nucleus in rat hypothalamic slices:Modulation by amino acids at cerebrospinal fluid levels." Brain.Res.691. 217-222 (1995)
Nishimura F:“大鼠下丘脑切片腹内侧核神经元的兴奋性:脑脊液水平氨基酸的调节。”
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- 影响因子:0
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KAWAMINAMI Mitsumori其他文献
KAWAMINAMI Mitsumori的其他文献
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{{ truncateString('KAWAMINAMI Mitsumori', 18)}}的其他基金
A role for Annexin A5 in the pregnancy
膜联蛋白 A5 在妊娠中的作用
- 批准号:
24658249 - 财政年份:2012
- 资助金额:
$ 4.86万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
A novel physiological function of mast cells on tissue remodeling
肥大细胞在组织重塑中的新生理功能
- 批准号:
23380175 - 财政年份:2011
- 资助金额:
$ 4.86万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Involvement of annexin 5 in G protein mediated signal transduction
膜联蛋白 5 参与 G 蛋白介导的信号转导
- 批准号:
14360181 - 财政年份:2002
- 资助金额:
$ 4.86万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
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