BRAIN MONOAMINES AND LUTEINIZING HORMONE SECRETION

脑单胺和黄体生成素的分泌

• 批准号: 3312271
• 负责人: WILLIAM R CROWLEY
• 金额: $ 10.04万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-12-01 至 1990-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3312271
• 关键词: 6 hydroxydopamine; brain metabolism; denervation; dopamine; epinephrine; estradiol; high performance liquid chromatography; hormone regulation /control mechanism; laboratory rat; luteinizing hormone; neurochemistry; neuroendocrine system; neuropeptide Y; neurotoxins; neurotransmitter metabolism; norepinephrine; optics; pituitary gonadal axis; progesterone; prolactin; radioimmunoassay; radiotracer; serotonin; sympathetic nervous system; thin layer chromatography; tritium

The objective is to examine the neural mechanisms by which the ovarian hormones, estradiol and progesterone, regulate the secretion of luteinizing hormone (LH) from the anterior pituitary gland. The experiments will test the hypothesis that the inhibitory and stimulatory feedback actions of the ovarian hormones are mediated in part via changes in the activity of noradrenergic and adrenergic neural systems that control the neurosecretion of LH-releasing hormone (LHRH). It is proposed that estradiol and progesterone affect activity in intrahypothalamic neuronal systems, such as the endogenous opioid neuropeptides and Gamma-aminobutyric acid, which regulate catecholamine release, and of neuropeptide Y (NPY), which modulates the response of the pituitary gland to LHRH. To test this hypothesis further, discrete hypothalamic areas will be incubated understatic or perifusion conditions in vitro. The release of LHRH and NPY into the medium will be measured by radioimmunoassays, while catecholamines will be measured by radioenzymatic assay. The first group of experiments will examine the postsynaptic mechanisms, especially the involvement of the Ca++ messenger system, mediating the stimulatory effect of norepinephrine (NE), epinephrine (EPI) and NPY on LHRH release. These studies will also test whether NPY alters the response of LHRH neurons to the catecholamines. A second set of studies will examine the interaction of NPY with the catecholamines in the regulation of LHRH release, and in particular, will test whether NE or EPI stimulates the release of NPY. A third set of studies will test whether the inhibitory effects of low dosage E2 treatment in vivo on the release of LHRH in vitro is due to activation of opioid systems that depress the release of NE and NPY. Other experiments will test whether the effect of in vitro progesterone to stimulate LHRH release is mediated by increased release of NE, EPI or NPY, and whether this can be inhibited by opioid or by GABA agonists. A fourth set of studies will test further the mechanisms underlying the facilitation of LHRH-induced LH release by NPY, using dispersed anterior pituitary cells in culture.

目的是研究卵巢癌的神经机制， 激素，雌二醇和孕酮，调节促黄体生成激素的分泌， 垂体前叶分泌的促黄体生成素（LH）。 实验将测试 这一假说认为，抑制性和刺激性的反馈行动， 卵巢激素部分是通过改变 控制神经分泌的去甲肾上腺素能和肾上腺素能神经系统 LH释放激素（LHRH）。 有人提出，雌二醇和 孕酮影响下丘脑内神经元系统的活性，例如 内源性阿片类神经肽和γ-氨基丁酸， 调节儿茶酚胺的释放，以及神经肽Y（NPY）， 调节脑下垂体对LHRH的反应。 为了验证这一 进一步假设，离散的下丘脑区域将被孵育 在体外静态或灌流条件下。 LHRH和NPY的释放 将通过放射免疫测定法测量进入培养基的量，而 将通过放射酶测定法进行测量。 第一组实验 将研究突触后机制，特别是参与 Ca++信使系统，介导去甲肾上腺素的刺激作用 (NE)、肾上腺素（EPI）和NPY对LHRH释放的影响。 这些研究还将 测试NPY是否改变LHRH神经元对 儿茶酚胺 第二组研究将探讨 在LHRH释放的调节中，NPY与儿茶酚胺一起起作用， 特别是，将测试NE或EPI是否刺激NPY的释放。 一 第三组研究将测试低剂量的抑制作用是否 E2在体内处理对体外LHRH释放的影响是由于激活 阿片系统抑制NE和NPY的释放。 其他 实验将测试是否在体外孕酮的效果， 刺激LHRH释放通过增加NE、EPI或NPY的释放来介导， 以及这是否可以被阿片类药物或GABA激动剂抑制。 第四 一组研究将进一步测试促进的机制 LHRH诱导的LH释放的NPY，使用分散的垂体前叶细胞 在文化中。