A Study on Accelerating Sytem of Aging in Brain Using 3-Dimensional Imaging Analysis Modified with Light Subtraction Method

本研究试图1)提取衰老加速小鼠（SAM）亚型（SAM - p8、P10和R1分别表现为学习记忆障碍、脑萎缩和对照组）在衰老过程中的行为和形态学变化特征；2)利用光减法修饰的超高速还原系统描述神经传递系统的动力学。3)测量衰老过程中大脑中微量元素的含量。本研究结果总结如下：1)与SAM-R1相比，SAM-P10和SAM-P8在9月龄和14月龄时出现了更明显的脱发和皮肤萎缩。与SAM-R1相比，SAM-P8在9、13月龄时的昼夜自发活动和体重显著降低，13月龄时的摄食量显著增加，SAM-P10与SAM-R1在4月龄时的体重和摄食量差异有增加的趋势。从这些时间序列数据中提取指数谱作为混沌存在的一个方面。2)采用光减法改进的三维成像分析，可以证实在短时间内保持高活性的新鲜切片的情况下，海马神经传递系统兴奋过程的可视化可以提供比以往方法更多的信息。3) icp -质谱分析发现，SAM-P10在9、12月龄时的锌浓度和6、9、12月龄时的铜浓度显著低于SAM-R1，而6、9、12月龄时的钼浓度显著高于SAM-R1。这些结果提示，SAM-P8、P10和R1在衰老过程中行为和神经生理方面的不同特征可能与它们大脑代谢系统的紊乱有关。需要进一步的研究来阐明产生这些差异的机制和系统。少

This study attempted 1) to extract the characteristics of behavioral and morphological changes in aging process of subtypes of senescence accelerated mice (SAM), which include SAM-P8, P10 and R1 characterized with disturbances of learning and memory, brain atrophy and control group respectively, 2) to describe the dynamics of neurotransmission system using ultra high speed reduction system modified with light subtraction method, and 3) to measure the trace elements in the aging process in their brains.The results of this study are summarized as follows.1) The alopecia and atrophy of skin were observed more clearly from 9 months age of SAM-P10 and from 14 months age of SAM-P8 compared with SAM-R1. Significantly lower spontaneous activity during night and daytime and body weight at 9 and 13 months age, and significantly more food intake at 13 months age in SAM-P8 compared to SAM-R1, tendency of increase of differences of body weight and food intake from 4 months age between SAM-P10 and S … More AM-R1 were observed. Exponential spectrum as an aspect of exitence of chaos was extracted from these time series data.2) Using 3-dimensional imaging analysis modified with light subtraction method, visualization of exciting process in the neurotransmission system in hippocampus in their brains could be confirmed to supply more information than previous methods, although only under the conditions of keeping fresh slices with high activities for short periods.3) Using ICP-mass spectroscopy, significantly less zinc concentration at 9 and 12 months of age and copper concentration at 6,9 and 12 months of age, and significantly higher molybdenum concentration at 6,9 and 12 months of age in cerebral cortex of SAM-P10 than of SAM-R1 were found.These results suggest that different characteristics in behavioral and neurophysiological aspects in aging process between SAM-P8, P10 and R1 may relate to disturbances of metabolic system in their brains. Further studies are necessary to clarify the mechanisms and systems which produce these differemces. Less