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A Study on Accelerating Sytem of Aging in Brain Using 3-Dimensional Imaging Analysis Modified with Light Subtraction Method

利用光减法改进的三维成像分析研究大脑加速衰老系统

基本信息

批准号：
07457091
负责人：
KOYAMA Akio
金额：
$ 4.86万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457091/
关键词：
Imaging analysis Visualization Aging Neurotransmission Hippocampus Time series analysis Exponential spectrum Chaos

项目摘要

This study attempted 1) to extract the characteristics of behavioral and morphological changes in aging process of subtypes of senescence accelerated mice (SAM), which include SAM-P8, P10 and R1 characterized with disturbances of learning and memory, brain atrophy and control group respectively, 2) to describe the dynamics of neurotransmission system using ultra high speed reduction system modified with light subtraction method, and 3) to measure the trace elements in the aging process in their brains.The results of this study are summarized as follows.1) The alopecia and atrophy of skin were observed more clearly from 9 months age of SAM-P10 and from 14 months age of SAM-P8 compared with SAM-R1. Significantly lower spontaneous activity during night and daytime and body weight at 9 and 13 months age, and significantly more food intake at 13 months age in SAM-P8 compared to SAM-R1, tendency of increase of differences of body weight and food intake from 4 months age between SAM-P10 and S … More AM-R1 were observed. Exponential spectrum as an aspect of exitence of chaos was extracted from these time series data.2) Using 3-dimensional imaging analysis modified with light subtraction method, visualization of exciting process in the neurotransmission system in hippocampus in their brains could be confirmed to supply more information than previous methods, although only under the conditions of keeping fresh slices with high activities for short periods.3) Using ICP-mass spectroscopy, significantly less zinc concentration at 9 and 12 months of age and copper concentration at 6,9 and 12 months of age, and significantly higher molybdenum concentration at 6,9 and 12 months of age in cerebral cortex of SAM-P10 than of SAM-R1 were found.These results suggest that different characteristics in behavioral and neurophysiological aspects in aging process between SAM-P8, P10 and R1 may relate to disturbances of metabolic system in their brains. Further studies are necessary to clarify the mechanisms and systems which produce these differemces. Less

本研究试图：1）提取加速衰老小鼠（SAM）各亚型（SAM-P8、P10和R1）在衰老过程中的行为学和形态学变化特征，分别以学习记忆障碍、脑萎缩和对照组为特征; 2）采用光减法改进的超高速还原系统描述神经传递系统的动力学，结果表明：（1）SAM-P10和SAM-P8在9月龄和14月龄时的脱毛和皮肤萎缩现象较SAM-R1明显，而SAM-R1在14月龄时的脱毛和皮肤萎缩现象较SAM-R1明显。与SAM-R1相比，SAM-P8在夜间和白天的自发活动和9月龄和13月龄的体重显著降低，而在13月龄的摄食量显著增加，SAM-P10和S之间的体重和摄食量差异从4月龄开始有增加的趋势 ...更多信息观察到AM-R1。从这些时间序列数据中提取出指数谱，作为混沌存在的一个方面。2）采用光减法改进的三维成像分析方法，可以证实其大脑海马神经传递系统兴奋过程的可视化比以往的方法提供了更多的信息。尽管仅在短时间内保持具有高活性的新鲜切片的条件下。3）使用ICP-质谱，9月龄和12月龄时锌浓度显著降低，6月龄、9月龄和12月龄时铜浓度显著降低，6月龄时钼浓度显著升高，结果表明，SAM-P8、P10和R1在衰老过程中行为和神经生理方面的不同特点可能与其脑内代谢系统紊乱有关。有必要进一步研究，以澄清产生这些症状的机制和系统。少