STUDIES ON THE MANNER OF T CELL ACTIVATION IN VARIOUS TYPES OF GLOMERULAR DISEASES

各类肾小球疾病中T细胞激活方式的研究

• 批准号: 11671025
• 负责人: KOYAMA Akio
• 金额: $ 2.3万
• 依托单位: UNIVERSITY OF TSUKUBA
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671025/
• 关键词: GLOMERUL ONEPHRITIS; NEPHROTIC SYNDROME; VASCULITIS; T CELL SUBSET; MANNER OF ACTIVATION; IMMUNE COMPLEX; SUPER ANTIGEN; IGA-RELATED NEPHRITIS; T細胞サブヒット; 免役複合体

In order to investigate the manner of T cell activation and usage of TCRV beta in GN, we analyzed the T cells of PBL from 129 patients with GN and 43 normal control by 3 color FACS, and classified them into 4 groups, according to the ratios of activated T cells and TCRV beta usage.In group 1 (proliferative IC-GN ; post-MRSA infectious GN, HSPGN), high ratios of activated T cells and increases of limited number of TCRV beta cells were observed. In group 2 (Autoimmune GN ; Lupus nephritis), high ratios of activated T cells and increases of various TCRV beta cells were observed. In group 3 (crescentic and vascular type ; idiopathic crescentic GN, microscopic PN), activated T cell ratio was significantly higher, however, several TCRV beta cells were significantly decreased. On the other hand, in group 4 (non-proliferative IC type ; membranous GN), there was no significant difference, compared to those in normal control.From the above observations, we speculate that manner of T cell activation and usage of TCRV beta are different in each group of glomerular disease, and T cells may contribute to the pathogenesis of glomerular diseases in the different manner.

为探讨GN患者外周血中T细胞活化和TCRVβ的利用情况，采用三色流式细胞仪分析129例GN患者和43例正常对照外周血中T细胞的活化情况，并根据T细胞活化和TCRVβ利用的比例将其分为4组。2组(自身免疫性肾炎；狼疮性肾炎)T细胞活化比例高，各种TCRVβ细胞增多。第3组(新月体型和血管型；特发性新月体肾炎、显微镜下肾炎)，活化T细胞比例明显升高，而少数TCRVβ细胞明显减少。而第4组(非增殖型IC型、膜性肾炎)与正常对照组比较无显著差异，推测各肾小球疾病组T细胞活化方式和TCRVβ的利用方式不同，T细胞可能以不同的方式参与肾小球疾病的发病。

项目成果

Yamagata K, et al.: "Prevalence of Japanese dialysis patients with A-to-G mutation at nucleotide 3243 of mitochondrial tRNA Leu (UUR) gene."Nephrology Dialysis Transplantation. 15. 385-388 (2000)

Yamagata K 等人：“日本透析患者线粒体 tRNA Leu (UUR) 基因第 3243 位核苷酸出现 A 至 G 突变的患病率。”肾脏病透析移植。

Yamagata K, et al: "Prevalence of Japanese dialysis patients with A-to-G mutation at nucleotide 3243 of mitochondrial tRNA Leu(UUR) gene."Nephrology Dialysis Transplantation. 15. 385-388 (2000)

Yamagata K 等人：“日本透析患者线粒体 tRNA Leu (UUR) 基因第 3243 位核苷酸出现 A 至 G 突变的患病率。”肾脏病透析移植。

Ebihara I, et al.: "predominancy of T helper subsets at the single-cell level in patients with IgA nephropathy."Nephrology. 5. A53 (2000)

Ebihara I 等人：“IgA 肾病患者单细胞水平上 T 辅助亚群的优势。”肾脏病学。

Muro K, et al.: "The usage of T cell receptor variable segments of the β chain in IgA nephropathy."Journal of American Society of Nephrology. 11. 72A (2000)

Muro K 等人：“T 细胞受体 β 链可变片段在 IgA 肾病中的应用。”美国肾病学会杂志 11. 72A (2000)。

小山 哲夫,小林 正貴: "最新内科学大系 プログレス10 腎泌尿器疾患:スーパー抗原関連腎炎"中山書店. 11 (1997)

Tetsuo Koyama、Masataka Kobayashi：“最新内科进展10肾脏和泌尿系统疾病：超抗原相关性肾炎”中山书店11（1997）。