BIOLOGICAL MONITORING OF EXPOSURE TOomicron-DICHLOROBENZENE

太微米二氯苯暴露的生物监测

• 批准号: 07457115
• 负责人: KUMAGAI Shinji
• 金额: $ 1.6万
• 依托单位: OSAKA PREFECTURAL INSTITUTE OF PUBLIC HEALTH,SCIENTIFIC RESEARCH
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457115/
• 关键词: omicron-DICHLOROBEZENE; URINARY METABOLITE; DICHLOROCATECHOL; DICHLOROPHENOL; BIOLOGICAL MONITORING

1) Identification of urinary metabolites of human subjects exposed to omicron-dichlobenzene (omicron-DCB)Urine samples were collected from three male workers exposed to omicron-DCB and they were analyzed using a gas chromatograph-mass spectrometer. 2,3-dichlorophenol (2,3-DCP), 3,4-dichlorophenol (3,4-DCP), 3,4-dichlorocatechol (3,4-DCC) and 4,5-dichlorocatechol (4,5-DCC) were detected in the all samples.2) Quantitative determination of urinary metabolites of omicron-DCB using a high performance liquid chromatograph (HPLC)First, urinary conjugated metabolites were hydrolyzed by concentrated hydrochloric acid, and the metabolites were extracted with diethylether. Next, 0.5 ml of the ethylether layr was separated, and 2.0 ml of methyl alcohol was added. The HPLC was connected to a column packed with LiChrospher 100 RP-18. For DCCs, the mobile phase was a mixture of acetonitrile, acetic acid and water (1 : 12 : 87). For DCPs, the mobile phase was a mixture of methyl alcohol, acetic acid a … More nd water (33 : 5 : 62). The effuents were monitored at 285 nm. The calibration curves were linear from 10 to 100 mg/l. The mean recovery rates of DCCs and DCPs were more than 90%.3) Relation between exposure to omicron-DCB and urinary metabolitesIn a plant symthesizing intermediate products for dyes, the 8-hr time weighted average (8-hr TWA) value for the exposure concentration of omicron-DCB and concetrations of urinary 3,4-DCC,4,5-DCC,2,3-DCP and 3,4-DCP were determined for 28 male workers. The concetrations of 3,4-DCC,4,5-DCC,2,3-DCP and 3,4-DCP inurine collected at the end of the workshift were shown to be linearly correlated to the 8-hr TWA value for the exposure concentration of omicron-DCB.Concequently, biological exposure monitoring can be done.4) Quantitative Determination of Urinary Metabolites of omicron-DCB Using a Gas Chromatograph (GC)Urine treatment was the same as that in the above HPLC method. The GC was connected to a capillary column was Shimadzu CBP-20. Helium was used as the carrier gas. The column temperature was programd from 50 to 220ﾟC at 3ﾟC/min. The injector and detector temperatures were 250ﾟC.The calibration curves were linear from 10 to 100mg/l. The mean recovery rates of 2,3-and 3,4-DCPs were more than 90%. Less

1)接触奥米克隆-二氯苯（omicron-DCB）的人体尿液代谢产物的鉴定收集了3名接触奥米克隆-DCB的男性工人的尿液样品，并使用气相色谱-质谱仪进行分析。在所有样品中均检测到2，3-二氯苯酚（2，3-DCP）、3，4-二氯苯酚（3，4-DCP）、3，4-二氯邻苯二酚（3，4-DCC）和4，5-二氯邻苯二酚（4，5-DCC）。2）采用高效液相色谱法（HPLC）定量测定Omicron-DCB的尿代谢产物首先，用浓盐酸水解尿结合代谢产物，用乙醚提取代谢产物。接着，分离出0.5ml乙醚层，并加入2.0ml甲醇。将HPLC连接至填充有LiChrospher 100 RP-18的柱。对于DCC，移动的相是乙腈、乙酸和水（1：12：87）的混合物。对于DCP，移动的相是甲醇、乙酸和乙酸的混合物， ...更多信息 水（33：5：62）在285 nm处监测流出物。校准曲线在10 - 100 mg/l范围内呈线性。在某染料中间体合成厂，测定了28名男工尿中3，4-DCC、4，5-DCC、2，3-DCP和3，4-DCP的浓度和接触Omicron-DCB的8小时时间加权平均值（8小时TWA）。结果表明，工人班末尿中3，4-DCC、4，5-DCC、2，3-DCP和3，4-DCP的浓度与接触Omicron-DCB的8小时TWA值呈线性相关。可以进行生物暴露监测。4）使用气相色谱仪（GC）定量测定omicron-DCB的尿代谢物尿处理与上述HPLC法相同。GC连接到Shimadzu CBP-20毛细管柱。使用氦气作为载气。柱温为50 ~ 220 ℃，进样口和检测器温度为250 ℃，流速为3 ℃/min，线性范围为10 ~ 100 mg/l。2，3-和3，4-DCP的平均回收率均大于90%。少

项目成果

Shinji Kumagai, Ichiro Matsunaga: "Identification of urinary metabolites of human subjects exposed to omicron-dichlorobenzene" Int Arch Occup Environ Health. 67. 207-209 (1995)

Shinji Kumagai、Ichiro Matsunaga：“暴露于 omicron-二氯苯的人体尿液代谢物的鉴定”Int Arch Occup Environ Health。

Shinji Kumagai: "Relations between exposure to o-dichlorobenzene and concentrations of urinary metabolites." J Occup Health. 39. 124-129 (1997)

Shinji Kumagai：“接触邻二氯苯与尿液代谢物浓度之间的关系。”