BIOLOGICAL MONITORING OF EXPOSURE TOomicron-DICHLOROBENZENE

1)对三名接触奥米克隆二氯苯（ommicron - dcb）的男性工人进行尿液代谢物鉴定，采用气相色谱-质谱联用仪对其进行分析。在所有样品中均检测到2,3-二氯苯酚（2,3- dcp）、3,4-二氯苯酚（3,4- dcp）、3,4-二氯儿茶酚（3,4- dcc）和4,5-二氯儿茶酚（4,5- dcc）。2)高效液相色谱（HPLC）定量测定ommicron - dcb尿液代谢产物首先，用浓盐酸水解尿偶联代谢产物，用二乙醚提取代谢产物。接下来，分离0.5 ml的乙醚层，加入2.0 ml的甲醇。HPLC连接到填充LiChrospher 100 RP-18的柱上。对于dcc，流动相为乙腈、乙酸和水的混合物（1:12:87）。对于dcp，流动相为甲醇、乙酸和水的混合物（33:5:62）。在285 nm处对流出液进行监测。在10 ~ 100 mg/l范围内，标定曲线呈线性。dcc和dcp的平均回收率均在90%以上。在某染料中间体合成工厂，测定了28名男性工蚁尿中3,4- dcc、4,5- dcc、2,3- dcp和3,4- dcp暴露浓度的8小时加权平均值（8小时TWA）。在工作结束时收集的3,4- dcc,4,5- dcc,2,3- dcp和3,4- dcp的浓度与8小时的TWA值的暴露浓度呈线性相关。因此，可以进行生物暴露监测。4)气相色谱仪（GC）定量测定尿中ommicron - dcb代谢物尿液处理与上述HPLC法相同。气相色谱连接至岛津CBP-20毛细管柱。氦气被用作载气。色谱柱温度设定为50 ~ 220ºC/min（3ºC/min）。进样器和检测器温度均为250℃。校准曲线在10 ~ 100mg/l范围内呈线性。2,3和3,4- dcp的平均回收率均大于90%。少

1) Identification of urinary metabolites of human subjects exposed to omicron-dichlobenzene (omicron-DCB)Urine samples were collected from three male workers exposed to omicron-DCB and they were analyzed using a gas chromatograph-mass spectrometer. 2,3-dichlorophenol (2,3-DCP), 3,4-dichlorophenol (3,4-DCP), 3,4-dichlorocatechol (3,4-DCC) and 4,5-dichlorocatechol (4,5-DCC) were detected in the all samples.2) Quantitative determination of urinary metabolites of omicron-DCB using a high performance liquid chromatograph (HPLC)First, urinary conjugated metabolites were hydrolyzed by concentrated hydrochloric acid, and the metabolites were extracted with diethylether. Next, 0.5 ml of the ethylether layr was separated, and 2.0 ml of methyl alcohol was added. The HPLC was connected to a column packed with LiChrospher 100 RP-18. For DCCs, the mobile phase was a mixture of acetonitrile, acetic acid and water (1 : 12 : 87). For DCPs, the mobile phase was a mixture of methyl alcohol, acetic acid a … More nd water (33 : 5 : 62). The effuents were monitored at 285 nm. The calibration curves were linear from 10 to 100 mg/l. The mean recovery rates of DCCs and DCPs were more than 90%.3) Relation between exposure to omicron-DCB and urinary metabolitesIn a plant symthesizing intermediate products for dyes, the 8-hr time weighted average (8-hr TWA) value for the exposure concentration of omicron-DCB and concetrations of urinary 3,4-DCC,4,5-DCC,2,3-DCP and 3,4-DCP were determined for 28 male workers. The concetrations of 3,4-DCC,4,5-DCC,2,3-DCP and 3,4-DCP inurine collected at the end of the workshift were shown to be linearly correlated to the 8-hr TWA value for the exposure concentration of omicron-DCB.Concequently, biological exposure monitoring can be done.4) Quantitative Determination of Urinary Metabolites of omicron-DCB Using a Gas Chromatograph (GC)Urine treatment was the same as that in the above HPLC method. The GC was connected to a capillary column was Shimadzu CBP-20. Helium was used as the carrier gas. The column temperature was programd from 50 to 220ﾟC at 3ﾟC/min. The injector and detector temperatures were 250ﾟC.The calibration curves were linear from 10 to 100mg/l. The mean recovery rates of 2,3-and 3,4-DCPs were more than 90%. Less