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Study on Pathological Process of Cerebral Venous Circulation Disturbance

脑静脉循环障碍的病理过程研究

基本信息

批准号：
07457321
负责人：
SAKAKI Toshisuke
金额：
$ 3.9万
依托单位：
Nara Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

Research on cerebral venous circulation disturbances (CVCD) has been limited partly by the lack of animal models that produce consistent venous infarction. We have reported that raty cortical vein occlusion by a photochemical thrombotic technique is a less invasite, clinically relevant and reproducible model. We here investigated microcirculation after venous occlusion and subsequent brain damage in this model.Experiment-1 : We evaluated changes of the cerebral venous flow pattern by fluorescence angiography, regional cerebral blood flow (rCBF) and cerebral blood volume (CBV) assessed by a modem laser Doppler "scanning" technique, and brain damage histologically in a rat cortical vein occlusion model using a photochemical thrombotic technique. Fluorescence angiographic findings could classify animals into 3 groups : (1)Group A,with a changed venous flow pattern after occlusion (n=12) ; (2)Group B,With an interruption of blood flow and/or a growing venous thrombus (n=5) ; (3)Group C : s … More ham-operated animals (n=5). Extravasation of fluorescein, a massive decrease of rCBF,a short-lasting increase of CBVF and regional brain damage were typical for group B.Cortical CBF mapping revealed a transient hyperperfusion zone with hyperemia surrounding a hypoperfused ischemic core in group B.Experiment-2 : We compared the results of two cortical veins occlusion group (T ; n=7) with those of the single vein occlusion with brain damage group (S ; n=5). rCBF reduction observed in group T,which occurred from 30 min post occlusion, was earlier and greater than decrese in group S from 60 min. Histogram obtained from data of group T demonstrated that local CBF of some locations decreased to a level below the ischemic threshold within 90 min. Six among 7 rats in group T had growing venous thrombus with extravasation of fluorescence and brain damage, and infarction size was bigger in group T than in group S.In conclusion, microciruclation perturbation appears very early following venous occlusion to result in the formation of venous thrombus accompanied by local critical ischemia and severe venous infarction. The extent of vein occlusion reflects the brain damage directly. Based on the results of this study, therapy for CVCD should include the prevention of formation of ongoing venous thrombus and ischemia. Less

脑静脉循环障碍（CVCD）的研究部分由于缺乏产生一致静脉梗塞的动物模型而受到限制。我们已经报道，通过光化学血栓形成技术进行的大鼠皮质静脉闭塞是一种侵入性较小、具有临床相关性且可重复的模型。我们在这里研究了静脉闭塞后的微循环以及该模型中随后的脑损伤。实验1：我们通过荧光血管造影评估了脑静脉血流模式的变化，通过现代激光多普勒“扫描”技术评估了局部脑血流量（rCBF）和脑血容量（CBV），并使用了大鼠皮层静脉闭塞模型中的组织学脑损伤光化学血栓技术。荧光血管造影结果可将动物分为3组：（1）A组，闭塞后静脉血流模式改变（n=12）； (2)B组，血流中断和/或静脉血栓生长(n=5)； (3)C组：火腿手术动物(n=5)。荧光素外渗、rCBF 大量减少、CBVF 短暂增加和局部脑损伤是 B 组的典型特征。皮质 CBF 测绘显示 B 组中缺血核心周围存在短暂的高灌注区，充血不足。实验 2：我们将两个皮质静脉闭塞组（T；n=7）的结果与单静脉闭塞组的结果进行比较脑损伤组（S；n=5）。 T组中观察到的rCBF减少是从闭塞后30分钟开始发生的，比S组从60分钟开始的减少更早且更大。从T组数据获得的直方图显示，一些部位的局部CBF在90分钟内下降至缺血阈值以下的水平。 T组7只大鼠中有6只出现静脉血栓生长，荧光外渗，脑损伤，梗死面积较S组大。综上所述，静脉闭塞后很早就出现微循环扰动，导致静脉血栓形成，并伴有局部严重缺血和严重静脉梗塞。静脉阻塞的程度直接反映脑损伤程度。根据这项研究的结果，CVCD 的治疗应包括预防持续静脉血栓和缺血的形成。较少的