Effect of volatile anesthetics on the ion-channels of the cerebral vascular smooth muscle isolated from dog

挥发性麻醉药对犬脑血管平滑肌离子通道的影响

• 批准号: 07457361
• 负责人: HATANO Yoshio
• 金额: $ 4.74万
• 依托单位: Wakayama Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457361/
• 关键词: Cerebral artery; Mesenteric artery; Ca^<++>-activated K^+ channel; Na^+-K^+ ATPase; Voltile anesthetics; 腸間膜動脈平滑筋; Na^+-K^+ ATPase; Ca依存性Kチャンネル; Na-K ATPase; カルシウムイオン; ナトリウムイオン

(1) The rings of the dog cerebral artery without endothelium but not the mesenteric artery maintained an automatic myogenic tone in the resting state. The myogenic tone was abolished in the Ca^<++> free Ringer solution and in the presence of nifedipine. These findings suggest that the increased basal Ca^<++> influx through voltage-dependent Ca^<++> channel is responsible for the maintenance of myogenic tone in the cerebral artery. Halothane significantly inhibited the myogenic tone observed in the cerebral arterial rings.(2) The addition of tetraethylammonium and charybdotoxin (Ca^<++>-activated K^+ channel inhibitors) produced a concentration-dependent contraction in the cerebral artery, but not in the mesenteric artery. These findings suggest that Ca^<++>-activated K^+ channel was highly activated by the increased Ca^<++> influx in the cerebral artery. Halothane, even at a low concentration of 0.5 MAC,strongly attenuated the tension development elicited by tetraethylammonium and charybdotoxin.(3) Exposure of the mesenteric arterial rings precontracted with phenylephrine to halothane (4 MAC) caused a rapid contraction, which reached a plateau within 5 to 10 min. After stabilization, termination of halothane produced a transient and profound relaxation. This relaxation was inhibited by ouabain, a Na^+-K^+ ATPase inhibitor, in a concentration-dependent manner. Substitution of Li^+ for Na^+ in Ringer solution also attenuated the relaxation. These results suggest that the relaxation elicited by termination of halothane exposure may be responsible to an alteration of Na^+-K^+ ATPaes activity in the mesenteric artery.The effect of volatile anesthetics on the alteration of intracellular ion concentration (Na^+, Ca^<++>) is now under way in our laboratory.

(1)狗的大脑动脉环无内皮，但不是肠系膜动脉在静息状态下保持自动肌源性紧张。在无Ca^<++>的林格氏液和硝苯地平存在下，肌源性张力被消除。这些结果表明，通过电压依赖性Ca^<++>通道增加的基础Ca^<++>内流是维持脑动脉肌源性张力的原因。氟烷显著抑制脑动脉环中观察到的肌源性张力。(2)加入四乙铵和Charybdotoxin（Ca^++激活的K^+通道抑制剂）后，脑动脉产生了浓度依赖性收缩，但肠系膜动脉没有。这些结果表明，脑动脉内Ca^<++>内流增加可高度激活Ca ^<++>激活的K^+通道。氟烷，即使在0.5 MAC的低浓度，强烈衰减的紧张局势的发展引起的四乙铵和charybdotoxin。(3)暴露的肠系膜动脉环预收缩苯乙双胍氟烷（4 MAC）引起快速收缩，达到一个平台期内5至10分钟。稳定后，终止氟烷产生短暂的和深刻的放松。哇巴因（一种Na^+-K^+ ATP酶抑制剂）可以浓度依赖性地抑制这种舒张作用。在林格溶液中用Li^+取代Na^+也减弱了弛豫。这些结果表明，终止氟烷暴露所引起的舒张作用可能与肠系膜动脉Na^+-K^+ ATP酶活性的改变有关。

项目成果

Iranami H, Hatano Y, et al.: "A beta-adrenoreceptor agonist evokes a nitric oxide-CGMP relaxation mechanism moduiated by adenylate cyclase in rat aorta" Anesthesiology. 85. 1129-1138 (1996)

Iranami H、Hatano Y 等人：“β-肾上腺素受体激动剂在大鼠主动脉中引起由腺苷酸环化酶调节的一氧化氮-CGMP 松弛机制”麻醉学。

Ogawa K.,et al.: "Difference Between Halcthane and Dentobarbital in Inhibition of the Contracfile Response to Hypocapnia and Bicarbonate in Isolated Canine Cerebral Artery" Anesthesiology. 83. A589 (1995)

Okawa K.,et al.：“Halcthane 和 Dentobarbital 在抑制离体犬脑动脉低碳酸血症和碳酸氢盐收缩反应方面的差异”麻醉学。

Yoshio, Hatano, Kazuhiro Mizumoto, Takeshi Yoshiyama, Manabu Yamamoto, Hiroshi Iranami: "Endothelium-dependent and -independent vasodilation of isolated rat aorta induced by caffeine" Am J Physiol. 269. H1679-1684 (1995)

Yoshio、Hatano、Kazuhiro Mizumoto、Takeshi Yoshiyama、Manabu Yamamoto、Hiroshi Iranami：“咖啡因诱导离体大鼠主动脉的内皮依赖性和非依赖性血管舒张”Am J Physiol。

Iranami H,Hatano Y,Tsukiyama Y,Maeda H,Mizumoto K: "A beta-adrenoreceptor agonist evokes a nitric oxide-cGMP relaxatoin mechanism modulated by adenylate cyclase in rat aorta" Anesthesiology. 85. 1129-1138 (1996)

Iranami H、Hatano Y、Tsukiyama Y、Maeda H、Mizumoto K：“β-肾上腺素受体激动剂在大鼠主动脉中引起由腺苷酸环化酶调节的一氧化氮-cGMP 松弛机制”麻醉学。

Yamamoto M, Hatano Y. et al: "Different effects of halothare, Isoflurone and sevaflurane on sarcoplasmic reticulum of vascular smooth muscle in dog mesenteric cutery" Acta Anaesth Scand. (in press).

Yamamoto M、Hatano Y. 等人：“halothare、异氟酮和七氟烷对狗肠系膜血管平滑肌肌浆网的不同影响”Acta Anaesth Scand。