INVESTIGATION ABOUT THE MECHANISM OF RETINAL ADHESION AND ITS BREAKDOWN AND RECOVERY

视网膜粘连及其破坏和恢复机制的研究

• 批准号: 07457413
• 负责人: NEGI Akira
• 金额: $ 4.8万
• 依托单位: KUMAMOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457413/
• 关键词: RETINA; RETINAL PIGMENT EPITHELIUM; RETINAL DETACHMENT; RETINAL BREAK; GAS TANPONADE; VITRECTOMY; SUBRETINAL FLUID; ガスタンポナ-デ; 黄班円孔; 硝子体; 中心性網膜炎; 網膜円孔

1 Healing process of the rabbit retinal pigment epithelium after mechanical removal A dome shaped retinal detachment was created in the rabbit eye by injecting saline into the subretinal space. RPE under the detached retina was removed from Bruck's membrane about 1.5mm in diameter and the healing process was observed morphologically. Shortly after the injury, RPE cells around the wound started to fill the defected area by sliding and migrating, and after 2 weeks the wound was completely covered. In eyes with overlying sensory retina, regenerated RPE cells formed a monolayr similar to normal RPE.The microvilli and RPE-photoreceptor outer segment interface was reorganized. In eyes with no sensory retina, the regenerated RPE were multilayred and the microvilli were short and disorganized. In many cases of damaged Bruck's membrane, choroidal neovascularization was observed.2 Sealing process of the retinal hole in the rabbit eyeRetinal hole of 0.5mm in diameter was made in the rabbit eye. After making artificial posterior vitreous detachment, vitrectomy and gas tamponade were performed. One week after surgery, the retinal hole was perfectly closed in all eyes macroscopically.Histologically, the hole was filled with glial cells, fibroblast and RPE.Diaminido yellow staining revealed that glial cells accumulated actively to the retinal wound and this process playd important roll to seal the hole.3 Effects of steroid on the subretinal fluid resorptionExperimental serous detachment was made by injecting rose bengal and mild photocoagulation in the rabbit eye. Preliminary results showed the tendency that in a group of administrating steroid, the serous detachment survived longer than that in non-administered group.

1兔视网膜色素上皮机械摘除后的愈合过程向视网膜下腔注入生理盐水造成兔眼穹隆状视网膜脱离。从直径约1.5 mm的Bruck膜上取出脱离视网膜下的RPE，并对愈合过程进行形态学观察。损伤后不久，创面周围的RPE细胞开始以滑动和迁移的方式填满缺损区，2周后创面完全覆盖。在有感觉性视网膜的眼，再生的RPE细胞形成与正常RPE相似的单层结构，微绒毛和RPE-光感受器外节界面重组。无感觉性视网膜者，再生的RPE多层，微绒毛短而杂乱。在许多Bruck膜受损的病例中，观察到脉络膜新生血管。2.在兔眼制作直径为0.5 mm的视网膜裂孔的封闭术。人工玻璃体后脱离后行玻璃体切割术和气体填充术。术后1周，所有兔眼视网膜裂孔完全闭合，组织学上可见裂孔内充满胶质细胞、成纤维细胞和视网膜色素上皮，二胺黄染色显示裂孔内胶质细胞活跃堆积，这一过程起到了封闭裂孔的重要作用。3类固醇对视网膜下液吸收的影响。初步结果显示，激素治疗组浆液性脱离存活时间较非激素治疗组延长。

项目成果

根木 昭: "接着機構から見た網膜疾患" 第一回国際眼科シンポジウム. 120-121 (1997)

Akira Negi：“从粘附机制看视网膜疾病”第一届国际眼科研讨会 120-121（1997 年）。

AKIRA NEGI: "RETINAL DISEASES INVESTIGATION FROM THE STANDPOINT OF ADHESION MECHANISM" THE FIRST INTERNATIONAL SYMPOSIUM OF OPHTHALMOLOGY,JAPAN OPHTHALMIC SOCIETY. 120-121 (1997)

AKIRA NEWI：“从粘附机制的角度进行视网膜疾病调查”第一届国际眼科研讨会，日本眼科学会。