权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Analysis of intraretinal neuro-glial interaction in glaucoma

青光眼视网膜内神经胶质细胞相互作用分析

基本信息

批准号：
14571672
负责人：
NEGI Akira
金额：
$ 2.37万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571672/
关键词：
glaucoma ocular hypertension retinal ganglion cell apoptosis glia グリア GFAP MAP1a

项目摘要

1.In an attempt to elucidate the causal effect of diabetes on development of glaucomatous optic neuropathy, male Sprague-Dawley rats were made diabetic by streptozotocin injection, in which unilateral eyes were treated with episcleral vein cauterization to produce chronically elevated intraocular pressure (IOP). Terminal dUTP nick end-labeling (TUNEL) staining was performed on the flat-mount retina dissected at several time points of sacrifice. The number of TUNEL positive cells in the inner retina was significantly higher in diabetic retina with episcleral vein 'cauterization than in control retina with same operation. Thus, diabetes has an additive effect on neuronal apoptosis induced by glaucoma in rats.2.In order to identify whether the pro-survival Akt pathway is activated in retina with chronically elevated IOP, immunohistochmeistry in the crysosection and in the whole mount retina as well as immunoblotting with retinal homogenates were conducted for rat retina with episcleral ve … More in cauterization. Akt phorphorylated at Ser 437 was specifically expressed in the retinal ganglion cells in the retina with cauterization, but not in the contralateral retina. Phosphorylated relative to total Akt was significantly increased in retina with cauterization. Thus, Akt activation may counteract against increased IOP stress in retina.3.Finally, to test whether glial reactivity was altered in glaucomatous retina, immunostaining and immunoblotting for glial fibrially acidic protein (GFAP) was conducted in the same experimental glaucoma model as in 2. Astrocytes lost and Muller cells gained GFAP imunoreactivity as early as at 3 days after episcleral vein cauterization, which was partially reversed overtime but last up to 6 months. GFAP molecular mobility and acidity were also changed by the cauterization. Similar observations were also occurred in the contrallateral retina at 1 month and thereafter. Thus, epicsleral vein cauterization altered glial reactivity not only in the ipsilateral but also in the contralateral eyes in rats. Less

1.为了阐明糖尿病对青光眼性视神经病变发展的因果影响，通过注射链脲佐菌素使雄性Sprague-Dawley大鼠患上糖尿病，其中单侧眼睛接受巩膜外静脉烧灼以产生慢性升高的眼内压（IOP）。在处死的几个时间点解剖的平装视网膜上进行末端 dUTP 缺口末端标记 (TUNEL) 染色。经巩膜静脉烧灼的糖尿病视网膜内层TUNEL阳性细胞数量明显高于经相同手术的对照视网膜。因此，糖尿病对大鼠青光眼诱导的神经细胞凋亡具有累加作用。 2.为了确定促生存Akt通路在慢性眼压升高的视网膜中是否被激活，对巩膜外层的大鼠视网膜进行了冰冻切片和全片视网膜的免疫组化以及视网膜匀浆的免疫印迹。烧灼。 Ser 437 磷酸化的 Akt 在烧灼视网膜的视网膜神经节细胞中特异性表达，但在对侧视网膜中不表达。烧灼后视网膜中相对于总 Akt 的磷酸化显着增加。因此，Akt 激活可能会抵消视网膜中增加的 IOP 应激。3.最后，为了测试青光眼视网膜中的神经胶质反应性是否发生改变，在与 2 相同的实验性青光眼模型中进行了神经胶质纤维酸性蛋白 (GFAP) 的免疫染色和免疫印迹。星形胶质细胞早在 3 天后就丢失了，而 Muller 细胞则获得了 GFAP 免疫反应性巩膜外静脉烧灼，随着时间的推移部分逆转，但持续长达 6 个月。烧灼也改变了 GFAP 分子的流动性和酸度。 1个月及之后的对侧视网膜也出现类似的观察结果。因此，巩膜上静脉烧灼不仅改变了大鼠同侧眼睛的神经胶质反应性，还改变了对侧眼睛的神经胶质反应性。较少的