Development of a small diameter vascular prosthesis with capacity of collateral formation to ischemic region due to angiogenesis

开发一种小直径血管假体，具有因血管生成而对缺血区域形成侧支的能力

• 批准号: 07457602
• 负责人: ENDO Masahiro
• 金额: $ 0.51万
• 依托单位: Tokyo Women's Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457602/
• 关键词: cytokine; grafts; vascular prostheses; bFGF; GABG; ischemic heart disease; collateral; blood vesselprosthesis; 冠動脈バイパス術

Basic fibroblast growth factor (bFGF) is one of the strongest angiogenic agent, and affects all cell types involved in would healing. The distribution of bFGF is widespread, because it plays an important role in regulating cell proliferation and mitosis. Angiogenesis by bFGF is important for endothelialization in vascular prostheses and is significant from shortly after implantation. Lack of angiogenic factors in the vascular graft wall may cause for unhealing neointima. Antithrombogenicity is important when the diameter of vascular prostheses become small for satisfactory patency. Natural and permanent antithrombogenicity due to host endothelial cells should be excellent. Collateral formation in ischemic heart disease is induced by angiogenic factors including bFGF.We hypothesized that tissue-fragmented (TF-) grafts are rich of angiogenic activity from shortly after implantation and it ability may work for collateral formation to the ischemic area when it is used for a small diameter vascular prosthesis in the coronary artery position. We developed a TF-graft and observed the healing process including angiogenesis and endothelialization. Proliferation and mitosis of endothelial cells ware active in the graft wall 5 days after implantation. The full thickness of the intima was organized at 7 days. These results demonstrated that endogenous bFGF is important for endothelialization due to angiogenesis in fabric vascular prostheses, whereas thrombus and infection might have a negative effect. It might be possible to have patent grafts in the coronary position, if we could have fine basic small-diameter fabric prosthesis with good handling characteristics, excellent antithrombogenicity.

碱性成纤维细胞生长因子（bFGF）是最强的血管生成因子之一，影响所有参与血管愈合的细胞类型。bFGF分布广泛，在调节细胞增殖和有丝分裂中起重要作用。bFGF的血管生成对于血管假体的内皮化具有重要意义，并且在植入后不久就具有重要意义。血管移植物壁缺乏血管生成因子可能导致新生内膜不愈合。当血管假体直径变小以获得满意的通畅时，抗血栓性是重要的。由于宿主内皮细胞的天然和永久的抗血栓性应该是优秀的。缺血性心脏病侧枝的形成是由血管生成因子包括bFGF诱导的。我们假设，组织碎片（TF-）移植物在植入后不久就具有丰富的血管生成活性，当它用于冠状动脉位置的小直径血管假体时，它的能力可能有助于缺血区域的侧支形成。我们开发了一种tf移植物，并观察了愈合过程，包括血管生成和内皮化。移植5天后，移植壁内皮细胞增殖和有丝分裂活跃。第7天内膜形成全层。这些结果表明，内源性bFGF对纤维血管假体血管生成的内皮化很重要，而血栓和感染可能有负面影响。在冠状动脉位置，如果我们能有良好的处理特性，良好的抗血栓性，细小的基本纤维假体，可能会有通畅的移植物。

项目成果

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