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Reactor Design for Biocellulose Production to the Aims of Industrial Process

以工业过程为目标的生物纤维素生产反应器设计

基本信息

批准号：
07555674
负责人：
TAKAI Mitsuo
金额：
$ 0.9万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

In this study, the most effective incubation methods for biocellulose production were examined in various mode of bioreactor, and then on the basis of the results, the bioreactor with rotating-disk has been designed. This is composed of horizontal pyrexcylinder of 20cm long and 15cm diameter, and stainless-made side plates equiped with one side of motor-drive, openings for culture medium and aeration, and with another side of pH and oxygen electrodes, two valves. Warmed water is passed through a thermojacked under the cylinder maintained at 28(]SY+-[)1ﾟC.pH in culture medium was automatically controlled by 1% NaOH aqueous solution. Aeration was done only into gas-phase by the filtered-air. Initial volume of the medium is 1800ml, that is half volume of the cylinder. Stainless mesh-disk from 6 to 16mesh of 14.4cm diameter were used as a supporter for bacterial cells. 60ml of preculture was inoculated into the reactor horizontally rotating 2-5rpm at 28ﾟC,and then incubated for 6days with aeration of 2000ml/min. The incubation was repeated 9times. Then total yealds of biocellulose has been examined for rotating speed and number of the disk, or pH control and aeration conditions. The most effective incubation conditions for the reactor of biocellulose production were as follows. 14mesh stainless disk was the most effective supporter for bacterial cells. Although the bioreactor produced about the same amount of biocellulose with respect to rotating speed of the disk, 30 and 50rpm, in the case of 50rpm incubation, there was some scattering of biocellulose too fast to retain on the disk. So the optimum rotating speed is 30rpm. Aeration into gas-phase would contribute to produce more biocellulose and to enhance physical properties of the biocellulose, especially, to increase in Young's modulus of the sheets.

本研究在不同的生物反应器模式下，考察了最有效的培养方法，并在此基础上设计了带培养盘的生物反应器。该装置由20 cm长、15 cm直径的水平耐热圆筒和不锈钢侧板组成，侧板一侧装有电机驱动装置、培养基和通气孔，另一侧装有pH和氧电极，两个阀门。使温水通过保持在280 ± 1 ℃的圆筒下的热夹套。通过1%NaOH水溶液自动控制培养基中的pH。通过过滤空气仅对气相进行曝气。培养基的初始体积为1800 ml，即圆筒体积的一半。用直径14.4cm的6 ~ 16目不锈钢网片作为细菌细胞的载体。将60 ml预培养物接种于28 ℃水平旋转2- 5 rpm的反应器中，然后以2000 ml/min的通气量培养6天，重复培养9次。然后对生物纤维素的总产量进行了检测，包括转速和圆盘数量，或pH控制和通气条件。生物纤维素生产反应器最有效的培养条件如下。14目不锈钢盘是细菌细胞最有效的载体。尽管生物反应器相对于盘的旋转速度（30和50 rpm）产生大约相同量的生物纤维素，但是在50 rpm孵育的情况下，存在一些太快而不能保留在盘上的生物纤维素的散射。因此，最佳转速为30 rpm。通气进入气相将有助于产生更多的生物纤维素，并提高生物纤维素的物理性能，特别是提高片材的杨氏模量。