Development of High Performance Temporarily Artificial Skin made from Salmon Collagen and Squid Chitin

以鱿鱼甲壳素悬浮液为原料，采用空气减压法制备了鱿鱼甲壳素片材，并将其作为临时人工皮肤的基材。用鲑鱼胶原蛋白将鱿鱼甲壳素片层压成层，用于人体皮肤细胞的生长。用鲑鱼胶原蛋白层合的甲壳素片材清楚地显示了人皮肤细胞与甲壳素片材的良好附着性，与用小牛胶原层压的甲壳素片材一样。人皮肤细胞在鲑鱼胶原复合甲壳素片材上的生长活性是小牛胶原片材的1.3倍，是单纯甲壳素片材的1.5倍。用DNA标记指数法确定鲑鱼胶原片层上生长的几丁质细胞为正常细胞。用鲑鱼胶原蛋白复合的甲壳素片材与小白鼠和兔进行了多次生物安全性试验，显示出较高的生物相容性。连续造纸机是为以鱿鱼甲壳素和鲑鱼胶原蛋白为原料制造临时人造皮肤而开发的。

The squid chitin sheet prepared from the squid chitin suspension by air-decompression was used as the base sheet for temporarily artificial skin. The squid chitin sheet was laminated with salmon collagen for the growth of human skin cells. The chitin sheet laminated with salmon collagen showed clearly the superior attachment of human skin cells to the chitin sheet only as well as the chitin sheet laminated with the calf collagen. The growth of human skin cells on the chitin sheet laminated with salmon collagen was activated 1.3 times than that on the sheet laminated with calf collagen and 1.5 times than that on the chitin sheet only. The growing cells on the chitin sheet laminated with salmon collagen were confirmed as the normal cells by the DNA labeling index method. The chitin sheet laminated with salmon collagen showed the high bio-compatibility on the several bio-safety tests with the mouse or the rabbit. The continuous paper machine was developed for the manufacturing of the temporarily artificial skin from the squid chitin and the salmon collagen.