Development of High Performance Temporarily Artificial Skin made from Salmon Collagen and Squid Chitin

开发由鲑鱼胶原蛋白和鱿鱼甲壳素制成的高性能临时人造皮肤

• 批准号: 09555294
• 负责人: TAKAI Mitsuo
• 金额: $ 7.49万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09555294/
• 关键词: Salmon collagen; Squid chitin; Temporarily artificial skin; Human skin cells; Attachment and growth of cells; DNA labeling index; Continuous paper machine; Bio-safety test; 連続抄紙機; 手漉き抄紙; 連続抄紙機試作; 上下リザーバー; 無終端ベルト; 繊維芽細胞; 接着・増殖

The squid chitin sheet prepared from the squid chitin suspension by air-decompression was used as the base sheet for temporarily artificial skin. The squid chitin sheet was laminated with salmon collagen for the growth of human skin cells. The chitin sheet laminated with salmon collagen showed clearly the superior attachment of human skin cells to the chitin sheet only as well as the chitin sheet laminated with the calf collagen. The growth of human skin cells on the chitin sheet laminated with salmon collagen was activated 1.3 times than that on the sheet laminated with calf collagen and 1.5 times than that on the chitin sheet only. The growing cells on the chitin sheet laminated with salmon collagen were confirmed as the normal cells by the DNA labeling index method. The chitin sheet laminated with salmon collagen showed the high bio-compatibility on the several bio-safety tests with the mouse or the rabbit. The continuous paper machine was developed for the manufacturing of the temporarily artificial skin from the squid chitin and the salmon collagen.

将鱿鱼甲壳素悬浮液经空气减压法制备鱿鱼甲壳素片作为临时人造皮肤的基片。鱿鱼甲壳素薄片与鲑鱼胶原蛋白层压，用于人体皮肤细胞的生长。与鲑鱼胶原蛋白层合的几丁质薄片清楚地表明，人类皮肤细胞与几丁质薄片的附着性优于与小牛胶原蛋白层合的几丁质薄片。人体皮肤细胞在三文鱼胶原层合的几丁质薄片上的生长活性是小牛胶原层合的1.3倍，是单独几丁质薄片的1.5倍。经DNA标记指数法证实，在三文鱼胶原层合的几丁质薄片上生长的细胞为正常细胞。三文鱼胶原层合甲壳素片在小鼠和家兔的生物安全性试验中均表现出较高的生物相容性。研制了以鱿鱼甲壳素和鲑鱼胶原蛋白为原料制备临时人造皮肤的连续造纸机。

项目成果

H.N. Cheng, M. Takai, et al.: "Rheology of carboxymethylcellulose made from bacterial cellulose"Macromol. Symp.. 140. 145-153 (1999)

H.N. Cheng、M. Takai 等人：“由细菌纤维素制成的羧甲基纤维素的流变学”Macromol。

K, Tajima et al.: "Enhancement of Bacterial Cellulose Productivity by Water-soluble Chitosan" Mokuzai Gakkaishi. 42(3). 279-288 (1996)

K, Tajima 等人：“水溶性壳聚糖增强细菌纤维素生产力”Mokuzai Gakkaishi。

K.Tajima et al.: "Synthesis of Bacterial Cellulose Composite (BCC) by Acetobacter xylinum, I" Mokuzai Gakkaishi. 41 (8). 749-757 (1995)

K.Tajima 等人：“木醋杆菌合成细菌纤维素复合材料 (BCC)，I”Mokuzai Gakkaishi。

M. Takai, et al.: "Development of temporarily artificial skin made from salmon collagen and squid chitin"Nihon Nogei Kagakukaishi. 72. 843-845 (1998)

M. Takai等：“开发由鲑鱼胶原蛋白和鱿鱼甲壳素制成的临时人造皮肤”Nihon Nogei Kagakukaishi。

高井 光男 他: "鮭皮コラーゲンとイカ甲キチンの代用皮膚開発"日本農芸化学会誌. 72(7). 843-845 (1998)

Mitsuo Takai 等人：“使用鲑鱼皮胶原蛋白和鱿鱼壳甲壳素开发替代皮肤”，日本农业化学学会杂志 72(7) (1998)。