Development of High Performance Temporarily Artificial Skin made from Salmon Collagen and Squid Chitin

将鱿鱼甲壳素悬浮液经空气减压法制备鱿鱼甲壳素片作为临时人造皮肤的基片。鱿鱼甲壳素薄片与鲑鱼胶原蛋白层压，用于人体皮肤细胞的生长。与鲑鱼胶原蛋白层合的几丁质薄片清楚地表明，人类皮肤细胞与几丁质薄片的附着性优于与小牛胶原蛋白层合的几丁质薄片。人体皮肤细胞在三文鱼胶原层合的几丁质薄片上的生长活性是小牛胶原层合的1.3倍，是单独几丁质薄片的1.5倍。经DNA标记指数法证实，在三文鱼胶原层合的几丁质薄片上生长的细胞为正常细胞。三文鱼胶原层合甲壳素片在小鼠和家兔的生物安全性试验中均表现出较高的生物相容性。研制了以鱿鱼甲壳素和鲑鱼胶原蛋白为原料制备临时人造皮肤的连续造纸机。

The squid chitin sheet prepared from the squid chitin suspension by air-decompression was used as the base sheet for temporarily artificial skin. The squid chitin sheet was laminated with salmon collagen for the growth of human skin cells. The chitin sheet laminated with salmon collagen showed clearly the superior attachment of human skin cells to the chitin sheet only as well as the chitin sheet laminated with the calf collagen. The growth of human skin cells on the chitin sheet laminated with salmon collagen was activated 1.3 times than that on the sheet laminated with calf collagen and 1.5 times than that on the chitin sheet only. The growing cells on the chitin sheet laminated with salmon collagen were confirmed as the normal cells by the DNA labeling index method. The chitin sheet laminated with salmon collagen showed the high bio-compatibility on the several bio-safety tests with the mouse or the rabbit. The continuous paper machine was developed for the manufacturing of the temporarily artificial skin from the squid chitin and the salmon collagen.