Development of High Performance Temporarily Artificial Skin made from Salmon Collagen and Squid Chitin

以鱿鱼甲壳素悬浮液为原料，采用空气减压法制备鱿鱼甲壳素片，作为临时性人工皮肤的基片。鱿鱼甲壳素片层与鲑鱼胶原蛋白层压用于人皮肤细胞的生长。与鲑鱼胶原层压的甲壳质片清楚地显示出人类皮肤细胞仅与甲壳质片以及与小牛胶原层压的甲壳质片的上级附着。人皮肤细胞在层压有鲑鱼胶原的甲壳质片上的生长比在层压有小牛胶原的片上的生长活化1.3倍，比仅在甲壳质片上的生长活化1.5倍。通过DNA标记指数法证实了在与鲑鱼胶原层压的甲壳素片上生长的细胞为正常细胞。甲壳素层压鲑鱼胶原蛋白的几个生物安全性测试与小鼠或兔显示出高的生物相容性。以鱿鱼甲壳素和鲑鱼胶原蛋白为原料，研制了一种用于制备临时性人工皮肤的连续抄纸机。

The squid chitin sheet prepared from the squid chitin suspension by air-decompression was used as the base sheet for temporarily artificial skin. The squid chitin sheet was laminated with salmon collagen for the growth of human skin cells. The chitin sheet laminated with salmon collagen showed clearly the superior attachment of human skin cells to the chitin sheet only as well as the chitin sheet laminated with the calf collagen. The growth of human skin cells on the chitin sheet laminated with salmon collagen was activated 1.3 times than that on the sheet laminated with calf collagen and 1.5 times than that on the chitin sheet only. The growing cells on the chitin sheet laminated with salmon collagen were confirmed as the normal cells by the DNA labeling index method. The chitin sheet laminated with salmon collagen showed the high bio-compatibility on the several bio-safety tests with the mouse or the rabbit. The continuous paper machine was developed for the manufacturing of the temporarily artificial skin from the squid chitin and the salmon collagen.