Development of multi-component sensor by using rotating bioreactor and stationary ring electrode/optical fiber

利用旋转生物反应器和静环电极/光纤开发多组分传感器

• 批准号: 07556097
• 负责人: MATSUMOTO Kiyoshi
• 金额: $ 0.32万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556097/
• 关键词: immobilized enzyme; multi-component sensor; biosensor; flow injection analysis; rotating bioreactor; fluorescence detection; chemiluminescence detection; 静止リング電極

An automated multi-channel flow analyzer equipped with biosensing devices was developed for multi-component measurement in various samples. Biosensing was achieved with single detector by the aid of an immobilized enzyme reactor installed in the channel, and the channel switching process for the sensing of a different compound was made by using a colum-switching rotary valve. Another rotary valve was used for auto-sampling. Subsequent measurements of glucose, sucrose and lactate were performed automatically in real samples.Then, an identical analyzing system was constructed for the rapid measurement of both the total amino acid content and the L-glutamate content of green tea infusions. L-Glutamate in samples was oxidized and deaminated enzymatically in a channel with a mini-reactor that was packed with L-glutamate oxidase immobilized on glass beads, and the evolved ammonium ions were measured by a fluorogenic reaction with omicron-phthalaldehyde and 2-mercaptoethanol. The total amino … More acid content was measured in a channel with another mini-reactor which was filled with immobilized L-amino acid oxidase. The analytical results showed that the canned green tea drinks examined were similar in terms of chemical composition in infusions of the Japanese green tea called sencha. The quality evaluation of green tea was possible to some extent by this method.Finally, an analyzing system by combined use of electrochemical and chemiluminescence/fluorescence detector was developed as a multi-component sensor system equipped with both a rotating bioreactor and an immobilized enzyme column. With electrochemical and chemiluminescence detector, semultaneous quantitation system of ethanol and glucose was achieved by use of an immobilized alcohol oxidase rotating bioreactor and an immobilized glucose oxidase column. Chemiluminescence of hydrogen peroxide produced by the reaction with alcohol oxidase was reacted with luminol and the chemiluminescence produced was detected. This system was applied to the quantitation of ethanol and glucose in Japanese sake and the results agreed well with F-kit method. With electrochemical and fluorescence detector, individual detections were achieved, but further investigations were needed for simultaneous quantitation. Less

研制了一种配备生物传感装置的多通道自动流动分析仪，用于多种样品的多组分测定。生物传感器实现与单一检测器的援助，固定化酶反应器安装在通道中，和通道切换过程中，用于传感不同的化合物，是通过使用柱切换旋转阀。另一个旋转阀用于自动取样。随后对真实的样品进行了葡萄糖、蔗糖和乳酸的自动检测，构建了一套快速测定绿色茶饮料中氨基酸总量和谷氨酸含量的分析系统。样品中的L-谷氨酸被氧化和脱氨基的酶在一个通道中的微型反应器，填充与L-谷氨酸氧化酶固定在玻璃珠，和释放的铵离子通过荧光反应测定与omicron-phthalaldehyde和2-巯基乙醇。总氨基酸 ...更多信息 在具有另一个填充有固定化L-氨基酸氧化酶的微型反应器的通道中测量酸含量。分析结果表明，所检测的罐装绿色茶饮料与日本绿色茶（称为森查）的冲泡液的化学成分相似。该方法在一定程度上可用于绿色茶叶的品质评价。最后，建立了一种电化学-化学发光/荧光检测器联用的多组分传感器系统，该系统由旋转生物反应器和固定化酶柱组成。采用固定化乙醇氧化酶旋转生物反应器和固定化葡萄糖氧化酶柱，以电化学和化学发光检测器实现了乙醇和葡萄糖的同时定量。利用乙醇氧化酶与过氧化氢反应产生的化学发光与鲁米诺反应，检测其化学发光。将该系统应用于日本清酒中乙醇和葡萄糖的测定，结果与F-kit法一致。通过电化学和荧光检测器，实现了单独检测，但同时定量还需要进一步研究。少

项目成果

Mei-Hwei Lee,Riche L.C.Chen Kiyoshi Matsumoto: "Fluorometric Biosensing of the Total Amino Acid Content and the Glutamate Content of Green Tea Infusions Using an Automated Multi-Channel Flow System" Bioscience Biotechnology and Biochemistry. 60・1. 99-102

Mei-Hwei Lee、Riche L.C.Chen Kiyoshi Matsumoto：“使用自动多通道流系统对绿茶输液的总氨基酸含量和谷氨酸含量进行荧光生物传感”生物科学生物技术和生物化学 60・1。

RichiL.C.Chen Kiyoshi Matsumoto: "Biosensing of Glucose,Sucrose,and Lactate in Beverages with an Automated Multi-channel Flow Analyzer" Bioscience Biotechnology and Biochemistry. 59・5. 813-816 (1995)

RichiL.C.Chen Kiyoshi Matsumoto：“使用自动多通道流量分析仪对饮料中的葡萄糖、蔗糖和乳酸进行生物传感”《生物科学生物技术和生物化学》59・5（1995）。

Mei-Hwei Lee, Riche L.C.Chen, and Kiyoshi Matsumoto: "Fluorometric Biosensing of the Total Amino Acid Content and the Glutamate Content of Green Tea Infusions Using an Automated Multi-Channel Flow System." Biosci. Biotech. Biochem.60-1. 99-102 (1996)

Mei-Hwei Lee、Riche L.C.Chen 和 Kiyoshi Matsumoto：“使用自动多通道流动系统对绿茶浸液的总氨基酸含量和谷氨酸含量进行荧光生物传感”。

Mei-Hwei Lee, Riche L.C.Chen, and Kiyoshi Matsumoto: "Novel Enzyme Assays for the Measurement of Gallotannin, Amino Acids, and Glutamate in Green Tea Infusion : Analytical Results." Biosci. Biotech. Biochem.60-2. 362-363 (1996)

Mei-Hwei Lee、Riche L.C.Chen 和 Kiyoshi Matsumoto：“用于测量绿茶浸液中没食子单宁、氨基酸和谷氨酸的新型酶测定：分析结果。”

Mei-Hwei Lee,Riche L.C.Chen Kiyoshi Matsumoto: "Novel Enzyme Assays for the Measurement of Gallotannin,Amino Acids,and Glutamate in Green Tea Infusion : Analytical Results" Bioscience Biotechnology and Biochemistry. 60・2. 362-363 (1996)

Mei-Hwei Lee、Riche L.C.Chen Kiyoshi Matsumoto：“用于测量绿茶浸液中没食子单宁、氨基酸和谷氨酸盐的新型酶测定：分析结果”生物科学生物技术和生物化学 60・2。