Establishment of a new inbred strain rat developing spontaneous eosinohilia (MES) and investigation of the pathogenesis.

自发性嗜酸性粒细胞增多症（MES）大鼠新近交系的建立及其发病机制的研究。

• 批准号: 12680808
• 负责人: MATSUMOTO Kiyoshi
• 金额: $ 2.05万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680808/
• 关键词: Eosinophil; Eosinophilia; Immunodeficiency; Rat; Parasitosis; Model animal; ConA肝炎

From our research, following results are obtained.1) A quite new strain of spontaneous eosinophilic rat (Matsumoto Eosinophilia shinshu rats: MES/sdi) was produced.2) All animals (more than 1500 rats tested) develop eosinophilia and bone marrow cell proliferation also observed.3) From the results of anti-IL-5 study, it is suggested that IL-5 plays an important roles in pathogenesis of eosinophilia.4) Histopathologically, eosinophilic proliferation and infiltration in the bone marrow, gastrointestinal tract, mesenteric lymph node, and spleen was found.5) MES rats were resistant to infection with T. spiralis newborn larvae. This indicates that eosinophils destroy the newborn larvae in vivo.From these results, it is concluded that MES rats could be a useful animal model for research on HES (Hypereosinophilic syndrome in humans) because the hypereosinophilia, with many associated eosinophilic lesions seen in the MES rats, is comparable to the features of HES in humans and other animals.

本研究获得以下结果：1）一种新的自发性嗜酸性粒细胞大鼠品系（松本嗜酸性粒细胞增多shinshu大鼠：MES/sdi）。2）所有动物（超过1500只测试的大鼠）发生嗜酸性粒细胞增多，骨髓细胞增殖也被抑制。3）从抗IL-5研究的结果来看，提示IL-5在嗜酸粒细胞增多症的发病机制中起重要作用。4）组织学上，骨髓、胃肠道、肠系膜淋巴结、5）MES大鼠对T.螺旋幼虫从这些结果可以得出结论，MES大鼠可能是一个有用的动物模型，用于研究HES（人类嗜酸性粒细胞增多综合征），因为在MES大鼠中观察到的嗜酸性粒细胞增多症，以及许多相关的嗜酸性粒细胞病变，与人类和其他动物的HES特征相当。

项目成果

Sano,K.,Matsumoto,K.: "A rat model of hypereosinophilic syndrome"Pathol.Int.. 51. 82-88 (2001)

Sano，K.，Matsumoto，K.：“嗜酸性粒细胞增多综合征的大鼠模型”Pathol.Int.. 51. 82-88 (2001)

Hiratochi,M.,Takamoto,M.: "Inhibition of interleukin 5 production with no influence on IL-4 production by an anti-allergic drug, tranilast, in TC-infectedmice."Int.J.Immunopharmacol.. 22(6). 463-471 (2000)

Hiratochi,M.,Takamoto,M.：“抗过敏药物曲尼司特在 TC 感染小鼠中抑制白细胞介素 5 的产生，但对 IL-4 的产生没有影响。”Int.J.Immunopharmacol.. 22(6)

Muto, S., Matsumoto, K.: "Systemic and eosinophilic lesions in rats with spontaneous eosinophilia"Vet. Pathol.. 38. 346-350 (2001)

Muto, S.、Matsumoto, K.：“自发性嗜酸性粒细胞增多大鼠的全身性和嗜酸性粒细胞病变”兽医。

Tagawa, Y., et al.: "Bimodal role of endogenous interleukin-6 in concanavalin A-induced hepatitis in mice."J. Leukoc. Biol.. 67. 90-96 (2000)

Takawa, Y. 等人：“内源性白细胞介素 6 在伴刀豆球蛋白 A 诱导的小鼠肝炎中的双模式作用。”J.

Tagawa, Y.et al.: "Differences between BALB/c and C57BL/6 in mouse hepatitis virus replication in primary hepatocyte culture"Exp.Anim.. 52. 81-84 (2003)

Takawa, Y.等人：“原代肝细胞培养物中小鼠肝炎病毒复制中 BALB/c 和 C57BL/6 之间的差异”Exp.Anim.. 52. 81-84 (2003)