Creative functional conversion of food resources with poor utility into alternative medical foods

将效用较差的食物资源创造性地功能转化为替代医疗食品

• 批准号: 11794007
• 负责人: MATSUMOTO Kiyoshi
• 金额: $ 5.18万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for University and Society Collaboration
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11794007/
• 关键词: Hypertenstion; Diabetes; Functional food design; adult disease

1. Postprandial suppression of blood glucose level (BGL) by anthocyanins.In order to optimize the postprandial BGL by food components for the prophylaxis of diabetes, various plants rich in authocyanins were investigated with respect to α-glucosidase (AGH) inhibition. As a result of in vitro screening on AGH inhibition, Ipomoea batatas cv Yamagawamurasaki purple sweet potato (YGM) had a potent maltase inhibition with the IC50 value of 0. 26 mg/ml. The YGM extract also showed a potent in vivo supressive effect of BGL after single oral administration of maltose in SD rats. As a result of isolation of active compound in YGM, acylated anthocyanins were found to show a potent AGH (maltase) inhibitory activity.2. Depressor mechanism of ACE inhibitory peptide (IVY) derived from wheat germ hydrolysate.In order to elucidate the depressor effect of Ile-Val-Tyr (IVY) isolated from wheat germ hydrolysate, in vivo ****hypertensive effect of it in SHR was primarily investigated. After intravenous ad … More iminstration of IVY (5mg/kg), a significant (P<0. 05) blood pressure (BP) reduction was observed at 8 min (ΔMBP=19. 2 mmHg), and the reduction was held for 20 min. By using rat and human plasma. IVY was found to be metabolized by the action of aminopeptidases in plasma to form a subsequent ACE inhibitor with hypotensive effect, VY. Thus, the intake of IVY or wheat germ hydrolysate would serve in reguating BP by the combined depressor effect of IVY and VY, after absorption.3. latent antihypertensive effect of buckwheat (BW) protein.Latent production of ACE inhibitors from tartary BW, and the characterization of peptides responsible fcr ACE inhibition were investigated. Sequential digestion with pepsin, trypsin and chymotrypsin resulted in a significant increase of ACE inhibitory activity (IC50 ; 0. 14 mg-protein/ml). rutin did not show the ACE inhibition. About 30 mmHg of BP reduction in SHR was observed after single oral adiministration of BW digest at 4 h. From the BW digest, 11 peptides were identified as an ACE inhibitor. The findings in this study demonstrated that BW protein had a latent ability to exert an in vivo antihypertensive effect after the gastrointestinal digestion process. Less

1.花青素对餐后血糖的抑制为了优化食物成分预防糖尿病的餐后血糖水平，研究了几种富含花青素的植物对α-葡萄糖苷酶的抑制作用。对山川紫甘薯(YGM)的AGH抑制作用进行了体外筛选，结果表明，YGM对麦芽糖酶有较强的抑制作用，IC50值为0。给SD大鼠灌胃麦芽糖后，银杏叶提取物对BGL有较强的体内抑制作用。通过分离YGM中的活性成分，发现酰化花色苷具有很强的AGH(麦芽糖酶)抑制活性。小麦胚芽水解物血管紧张素转换酶抑制肽(Ivy)的降压机制为阐明小麦胚芽水解物Ile-Val-Tyr(Ile-Val-Tyr，Ile-Val-Tyr)的降压作用，对其体内降压作用进行了初步研究。静脉注射ad…后常春藤(5 mg/kg)灌胃越多，差异显著(P&lt；0。05)观察8分钟血压下降(Δ平均血压=19.2毫米汞柱)，并保持20分钟。通过使用大鼠和人的血浆。常春藤被发现是通过血浆中氨基肽酶的作用来代谢的，形成随后的具有降压作用的ACE抑制剂VY。因此，常春藤或小麦胚芽水解物的摄入可能通过常春藤和VY的联合降压作用，在吸收后起到调节血压的作用。荞麦(BW)蛋白潜在的降压作用。研究了苦荞蛋白潜在的ACE抑制剂的产生，以及与FCR ACE抑制相关的多肽的特性。胃酶、胰酶和胰凝乳酶的顺序消化可显著提高血管紧张素转换酶抑制活性(IC50；14 mg-蛋白质/ml)。芦丁对血管紧张素转换酶无抑制作用。单次口服BW消化液4h后，SHR血压下降约30 mm Hg，从BW消化液中鉴定出11个多肽为ACE抑制物。本研究结果表明，BW蛋白在胃肠道消化过程中具有潜在的体内降压作用。较少

项目成果

T.Matsui, et al.: "α-Glucosidase Inhibitory Action of Natural Acylated Anthocyanins(1)"Journal of Agricultural and Food Chemistry. 49・4. 1948-1951 (2001)

T.Matsui等人：“天然酰化花青素的α-葡萄糖苷酶抑制作用(1)”农业和食品化学杂志49·4（2001）。

T. Matsui, M. Kobayashi, S. Hayashida, and K. Matsumoto: "Luteolin, a flavone, does not suppress postprandial glucose absorption through an inhibition of α-glucosidase action."Biosci. Biotechnol. Biochem.. (in press).

T. Matsui、M. Kobayashi、S. Hayashida 和 K. Matsumoto：“木犀草素是一种黄酮，不会通过抑制 α-葡萄糖苷酶作用来抑制餐后吸收。”Biosci. Biotechnol。 。

T. Matsui, T. Ueda, T. Oki, K. Sugita, N. Terahara, and K. Matsumoto: "α-Glucosidase Inhibitory Action of Natural Acylated Anthocyanins. 1. Survey of Natural Pigments with Potent Inhibitory Activity"J. Agric. Food Chem.. 49(4). 1948-1951 (2001)

T. Matsui、T. Ueda、T. Oki、K. Sugita、N. Terahara 和 K. Matsumoto：“天然酰化花青素的 α-葡萄糖苷酶抑制作用。1. 具有有效抑制活性的天然色素的调查”J. Agric食品化学. 49(4). 1948-1951 (2001)

T.Matsui, et al.: "Luteolin, a flavone, does not suppress postprandial glucose absorption through an inhibition of α-glucosidase action"Bioscience, Biotechnology, and Biochemistry. (in press).

T. Matsui 等人：“木犀草素是一种黄酮，不会通过抑制 α-葡萄糖苷酶作用来抑制餐后葡萄糖吸收”《生物科学、生物技术和生物化学》（正在出版）。