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ESTABLISHMENT OF THE DEVELOPING AND SUPPLING SYSTEMS FOR NEW ANIMAL,DISEASE MODELS FROM SUBSPECIES OF THE MOUSE

小鼠亚种新动物、疾病模型的开发和供应体系的建立

基本信息

批准号：
07556128
负责人：
NAMIKAWA Takao
金额：
$ 3.71万
依托单位：
NAGOYA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556128/
关键词：
diabetes mellitus NIDDM mutant strain biological rhythm circadian rhythm mouse chromosome mapping candidate gene 行動異常糖尿病モデル突然変異遺伝子実験動物

项目摘要

(1) A new animal model for non-insulin dependent diabetes mellitus from Philippine wild mouse, Mus musculus castaneus1) Intraperitoneal glucose tolerance test (IGT) showed that the males showed a higher incidence of impared glucose tolerance than females. The incidence of IGT was -50% in males among the lines being maintained. There seems to be no increase in the degree of severity in IGT between F4 and F5 individuals.2) Insulin secretory capacity of IGT mice was impaired with poor response to glucose stimulation. The control mice (C57BL/6J) showed a 2.5 times increase in insulin upon glucose injection (1.37mg/dl to 2.62mg/dl). However, there was small rise in serum insulin 0.5 h after stimulation in the IGT males.3) Triglyceride (TG) levels in the IGT mice was slightly higher than that in the control mice although it showed a large range in variation since sample collection was done in the fed state. The TG levels vary with the state (fasted/nonfaste) of sampling. On the other hand, total coresterol (TC) levels in the mice was generally higher (174-250 mg/dl) with values twicw that of the control mice (132 mg/dl).(2) A mutant was found in the Philippine wild mice, those mice exhibit no rhythmic circadian activity under the constant dark condition. The trait was found to be controled by a recessive gene with nearly 100% penetrance.

(1)本文报道了一种新的非胰岛素依赖型糖尿病动物模型-菲律宾小家鼠（Mus musculus castaneus）。1）腹腔内葡萄糖耐量试验（IGT）显示，雄性小鼠的IGT发生率高于雌性小鼠。在维持的品系中，IGT的发生率在雄性中为约50%。F4和F5个体间IGT的严重程度似乎没有增加。2）IGT小鼠的胰岛素分泌能力受损，对葡萄糖刺激的反应较差。对照小鼠（C57 BL/6 J）在注射葡萄糖后显示胰岛素增加2.5倍（1.37mg/dl至2.62mg/dl）。然而，在IGT雄性中，在刺激后0.5h血清胰岛素略有升高。3）IGT小鼠中的甘油三酯（TG）水平略高于对照小鼠中的TG水平，尽管其显示出较大的变化范围，因为样品是在进食状态下进行的。TG水平随采样状态（空腹/非空腹）而变化。另一方面，小鼠中的总胆固醇（TC）水平通常较高（174-250 mg/dl），是对照小鼠（132 mg/dl）的两倍。(2)在菲律宾野生小鼠中发现了一种突变体，该突变体小鼠在恒定黑暗条件下不表现出节律性昼夜活动。该性状受一对近100%隐性基因控制。