Developement of optical coherence tomography and its ophthalmologic application

光学相干断层扫描技术的发展及其眼科应用

• 批准号: 07557111
• 负责人: KINOSHITA Shigeru
• 金额: $ 7.3万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07557111/
• 关键词: light scattering; tomography; cornea; sclera; Michelson interferometer; optical coherence tomography; 断層線撮影

We established a prototype device of optical coherence tomography for ophthalmologic application in order to obtain noninvasively high-resolution tomographic images of anterior part of eyes. This device was built by the Michelson's theory using low-coherence infrared light. First, we investigated the most appropriate engineering set-up regarding light pass, super luminescent diode, beam-splitter, mirror etc., for this device, and finally achieved the depth resolution under 9 mum and the detectable level over 100 dB.After numerous improvements in the prototype device, we applied this to pig corneas to obtain A-mode images. A-mode images disclosed fine corneal structures, and the corneal thickness measured by this device was well correlated with that of ultrasonic pachometry. We further obtained fine B-mode images manually from central corneas with or without epithelium. Thus, we could differentiate abraded corneas from normal corneas using this device. The corneo-scleral junctional area showed a gradual change of scattering lights from corneal to scleral side. As a result, we have obtained almost all technical information regarding optical coherence tomography for the anterior segment of the eye. Therefore, we are now planning to make a second generation of optical coherence tomography for clinically applying human corneas and scleras.

我们建立了一种用于眼科应用的光学相干断层扫描原型设备，以获得眼睛前部的无创高分辨率断层扫描图像。该装置是根据迈克尔逊理论使用低相干红外光构建的。首先，我们研究了最适合该设备的光通、超发光二极管、分光镜、反射镜等工程设置，最终实现了9μm以下的深度分辨率和100dB以上的可检测水平。经过对原型设备的多次改进，我们将其应用于猪角膜以获得A模式图像。 A模式图像揭示了精细的角膜结构，并且该设备测量的角膜厚度与超声角膜厚度测量的结果具有良好的相关性。我们进一步从有或没有上皮的中央角膜手动获得了精细的 B 模式图像。因此，我们可以使用该设备区分磨损的角膜和正常角膜。角巩膜交界处显示散射光从角膜侧到巩膜侧逐渐变化。由此，我们获得了几乎所有关于眼前节光学相干断层扫描的技术信息。因此，我们现在正计划制作第二代光学相干断层扫描技术，用于临床应用人类角膜和巩膜。