Development of Diagnosis System for Atherosclerosis Based on the Autofluorescence of Blood and Vascular Walls

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We have performed experiments to examine the relationships between autofluorescence of blood and vascular walls of guinea pig atherosclerotic model and human patients and obtained the following results.1.The autofluorescence from human atherosclerotic aorta included the components with longer wave length than normal aorta, suggesting that diagnosis of atherosclerotic aortic walls will be possible using spectroscopic analysis through glass fiber catheter into vascular system. Further studies should be needed to the quantitative diagnosis.2.The autofluorescence from blood plasma of human atherosclerotic patients has showed that the peak wave length was shorter than that of normal plasma. This phenomenon was mainly caused by the oxidization of plasma, especially lipoproteins, low density lipoproteins (LDL) and high density lipoproteins (HDL) .3.Atherosclerotic model of the guinea pigs was quite similar to human atherosclerosis at the points of cholesterol levels and localization of lipid deposit to arterial walls, and showed to be useful for the studies of atherosclerosis. Autofluorescence analysis on blood plasma, red cells and white cells of guinea pigs was performed. Plasma of cholesterol-fed guinea pig had stronger autofluorescence with shorter wavelength at the peak, being similar to human plasma. Autofluorescence from red and white cells increased with cholesterol. Especially the autofluorescence materials from red cells was confirmed to be protoporpnyrin. Further, we measured sudanophilic area in aorta and found that the area had significant correlation with the strength of autofluorescence from plasma and red cells.