Development of Diagnosis System for Atherosclerosis Based on the Autofluorescence of Blood and Vascular Walls

我们通过实验研究了豚鼠动脉粥样硬化模型和人类患者血液和血管壁的自发荧光之间的关系，得到了以下结果：1.人动脉粥样硬化的自发荧光中含有比正常动脉波长更长的成分，提示通过玻璃纤维导管进入血管系统进行光谱分析将有可能诊断动脉粥样硬化性主动脉壁。定量诊断尚需进一步研究。2.人动脉粥样硬化患者血浆的自体荧光显示其峰值波长较正常血浆短。这种现象主要是由于血浆氧化，尤其是脂蛋白、低密度脂蛋白和高密度脂蛋白的氧化所致。3.豚鼠动脉粥样硬化模型在胆固醇水平和脂质沉积到动脉壁的位置上与人类动脉粥样硬化非常相似，为动脉粥样硬化的研究提供了有用的依据。对豚鼠血浆、红细胞和白细胞进行自体荧光分析。胆固醇喂养的豚鼠血浆具有较强的自发荧光，峰值波长较短，与人血浆相似。红细胞和白细胞的自发荧光随着胆固醇的增加而增加。特别是从红细胞中提取的自体荧光物质被证实为原甲鸟蛋白。此外，我们测量了主动脉中的嗜苏丹酸面积，发现该面积与血浆和红细胞的自发荧光强度有显著的相关性。

We have performed experiments to examine the relationships between autofluorescence of blood and vascular walls of guinea pig atherosclerotic model and human patients and obtained the following results.1.The autofluorescence from human atherosclerotic aorta included the components with longer wave length than normal aorta, suggesting that diagnosis of atherosclerotic aortic walls will be possible using spectroscopic analysis through glass fiber catheter into vascular system. Further studies should be needed to the quantitative diagnosis.2.The autofluorescence from blood plasma of human atherosclerotic patients has showed that the peak wave length was shorter than that of normal plasma. This phenomenon was mainly caused by the oxidization of plasma, especially lipoproteins, low density lipoproteins (LDL) and high density lipoproteins (HDL) .3.Atherosclerotic model of the guinea pigs was quite similar to human atherosclerosis at the points of cholesterol levels and localization of lipid deposit to arterial walls, and showed to be useful for the studies of atherosclerosis. Autofluorescence analysis on blood plasma, red cells and white cells of guinea pigs was performed. Plasma of cholesterol-fed guinea pig had stronger autofluorescence with shorter wavelength at the peak, being similar to human plasma. Autofluorescence from red and white cells increased with cholesterol. Especially the autofluorescence materials from red cells was confirmed to be protoporpnyrin. Further, we measured sudanophilic area in aorta and found that the area had significant correlation with the strength of autofluorescence from plasma and red cells.