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Measurement of mechanical properties of vascular smooth muscle and blood vessel wall as a model of functional materials

作为功能材料模型测量血管平滑肌和血管壁的机械性能

基本信息

批准号：
16360052
负责人：
MATSUMOTO Takeo
金额：
$ 9.66万
依托单位：
Nagoya Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16360052/
关键词：
Biomechanics Cell mechanics Vascular mechanics Vascular smooth muscle cells Viscoelastic analysis Stress strain distribution Mechanical adaptation 応力・ひずみ分布

项目摘要

To estimate mechanical environment of smooth muscle cells (SMCs) in the vessel wall, we measured mechanical properties of SMCs and their microscopic residual stress and strains in the wall : We measured quasi-static tensile properties of SMCs freshly isolated from the media with our original tensile tester to find that their mechanical properties have a close correlation with intracellular actin filaments (AFs). We also measured their stress relaxation properties to find that stress relaxation process could be separated into two phases : fast passive relaxation phase with a time constant in the order of a minute and slow active phase with a time constant in the order of an hour. Then, we established a method to elucidate microscopic mechanical environment for the aortic walls composed of concentric layers of SMCs and elastic laminas (ELs). To estimate stresses in the ELs and SMCs in the in vivo state, ELs were isolated enzymatically from 10-μm-thick slices of aortic walls to obtain their tensile properties. Upon isolation, corrugation of the ELs almost disappeared, indicating that the corrugation was buckling of the ELs due to their compressive residual strain. Residual stress and strain in the SMCs were estimated in their tensile test by referring to their nuclear length. Tensile stress required to restore the nuclear length in tissue and its concomitant strain was obtained as microscopic residual stress and strain and was found to be 10 kPa and 23%, respectively. In vivo stresses in the ELs and SMCs were calculated from data obtained in the present study and strain distribution obtained for homogeneous aortic wall. They were 80-40% of the mean hoop stress of the wall. Such inconsistency might be caused because 1) mechanical properties of SMCs were measured under fully relaxed state, and 2) collagen fibers were not taken into consideration.

为了估计血管壁中平滑肌细胞（SMC）的机械环境，我们测量了 SMC 的机械性能及其在壁中的微观残余应力和应变：我们用我们原来的拉伸测试仪测量了新鲜从培养基中分离的 SMC 的准静态拉伸性能，发现它们的机械性能与细胞内肌动蛋白丝（AF）密切相关。我们还测量了它们的应力松弛特性，发现应力松弛过程可以分为两个阶段：时间常数约为一分钟的快速被动松弛阶段和时间常数约为一小时的缓慢主动阶段。然后，我们建立了一种方法来阐明由 SMC 和弹性层（EL）同心层组成的主动脉壁的微观机械环境。为了估计 EL 和 SMC 在体内状态下的应力，通过酶法从 10 μm 厚的主动脉壁切片中分离出 EL，以获得其拉伸特性。隔离后，EL 的波纹几乎消失，表明波纹是由于 EL 的压缩残余应变而导致的屈曲。 SMC 中的残余应力和应变是在拉伸测试中根据其核长度进行估计的。恢复组织中核长度所需的拉应力及其伴随应变以微观残余应力和应变的形式获得，结果分别为 10 kPa 和 23%。 EL 和 SMC 的体内应力是根据本研究中获得的数据和均匀主动脉壁获得的应变分布计算得出的。它们是墙平均环向应力的 80-40%。造成这种不一致的原因可能是：1）SMC的机械性能是在完全松弛状态下测量的，2）没有考虑胶原纤维。

项目成果

期刊论文数量（38）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

細胞用二軸繰返引張機構の開発とこれを用いた血管内皮細胞の力学応答の解析

细胞双轴循环张力机制的开发及利用该机制分析血管内皮细胞的机械响应

DOI：
发表时间：
2004
期刊：
日本機械学会論文集A編 70・694
影响因子：
0
作者：
Nagayama K;Matsumoto T;長山和亮;中垣将
通讯作者：
中垣将

Tensile properties of cultured aortic smooth muscle cells obtained in a quasi-in situ tensile test with thermoresponsive gelatin

用热敏明胶进行准原位拉伸试验获得的培养主动脉平滑肌细胞的拉伸特性

DOI：
发表时间：
2006
期刊：
Journal of Biomechanical Science and Engineering 1・1
影响因子：
0
作者：
Nagayama K;Yanagihara S;Matsumoto T;松本健郎;Matsumoto T;Nagayama K
通讯作者：
Nagayama K

細胞の力学特性計測のためのレーザ顕微鏡複合型原子間力顕微鏡(AFM)システムの開発

开发用于测量细胞机械特性的激光显微镜组合原子力显微镜（AFM）系统

DOI：
发表时间：
2004
期刊：
日本機械学会論文集C編 70・691
影响因子：
0
作者：
Nagayama K;Matsumoto T;長山和亮
通讯作者：
長山和亮

Mechanical anisotropy of rat aortic smooth muscle cells decreases with their contraction - (Possible effect of actin filament orientation)

DOI：
10.1299/jsmec.47.985
发表时间：
2004-12-01
期刊：
JSME INTERNATIONAL JOURNAL SERIES C-MECHANICAL SYSTEMS MACHINE ELEMENTS AND MANUFACTURING
影响因子：
0
作者：
Nagayama, K;Matsumoto, T
通讯作者：
Matsumoto, T

Observation of Cell Shortening and Dynamic Changes of Actin Filaments during Cell Detachment from Thermoresponsive-Gelatin-Coated Substrate

细胞从热敏明胶涂层基质上脱离过程中细胞缩短和肌动蛋白丝动态变化的观察