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Bioinorganic chemical studies on behavior of selenium and other metals by the use of multielemental analysis.

利用多元素分析对硒和其他金属的行为进行生物无机化学研究。

基本信息

批准号：
07640742
负责人：
AMANO Ryohei
金额：
$ 1.47万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07640742/
关键词：
multitracer experiment neutron activation analysis trace element, V,Mn, Rb regional cerebral tracer uptake regional cerebral concentration brain function age effect mouse experiment マルチトレーサ法中性子放射化分析法脳機能ラジオアイソトープセレンバナジウム骨集

项目摘要

This study was undertaken in order to acquire better understanding of the biobehavior of selenium and other trace metals by the techniques of multitracer and instrumental neutron activation analysis. The followings were found.1). The radioactive multitracer technique, which was established at RIKEN,enables simultaneous tracing of a number of elements in a variety of bio-systems and an accurate comparison of their biobehavior. In this work, the multitracer technique was thus shown to be useful for comparative studies of behavior of trace elements in living bodies.2). We find new facts using the radioactive multitracer technique. (a) V is a bone-seeking element probably playing an role in bone metabolism. (b) Rubidium uptake rates for all soft tissues of mice housed under a 10% O_2 atmosphere were found to be higher than those of the tissues of mice housed under the other three O_2 atmospheres (20%, 80%, and 100%), but no difference was observed for the other elements (Sc, Mn, Fe, Co, Se, etc.) under any of O_2 the atmospheres. (c) Regional cerebral uptake behaviors of Mn, Fe, Zn and Se trace elements were determined in normal C57BL/6N mice, by using a quadruple radioactive tracers. In all regions the uptake rates were found in the following order ; Zn > Mn > Fe >Se.3). Form the technique of instrumental neutron activation analysis for small amount of regional samples. No difference in regional concentration was found in the brain of young and aged mice for the elements, Na, Mg, Cl, K,Rb and Br. On the other hand, significant differences were found for the element, Mn, in all regions of the brain of young and aged mice.

进行这项研究是为了通过多重示踪剂和仪器中子活化分析技术更好地了解硒和其他痕量金属的生物行为。发现如下：1)． RIKEN建立的放射性多重示踪剂技术能够同时追踪各种生物系统中的多种元素并准确比较它们的生物行为。在这项工作中，多重示踪剂技术被证明可用于活体中微量元素行为的比较研究。2)。我们使用放射性多重示踪剂技术发现了新的事实。 (a) V是一种寻骨元素，可能在骨代谢中发挥作用。 (b) 10% O_2 气氛下饲养的小鼠所有软组织对铷的吸收率均高于其他三种 O_2 气氛（20%、80% 和 100%）下饲养的小鼠组织的铷吸收率，但其他元素（Sc、Mn、Fe、Co、Se 等）在任何 O_2 气氛下均未观察到差异。 (c) 使用四重放射性示踪剂测定正常 C57BL/6N 小鼠中 Mn、Fe、Zn 和 Se 微量元素的区域脑摄取行为。在所有地区，采用率按以下顺序排列： Zn>Mn>Fe>Se.3)。形成少量区域样品的仪器中子活化分析技术。在年轻和老年小鼠的大脑中，Na、Mg、Cl、K、Rb 和 Br 元素的区域浓度没有差异。另一方面，在年轻和老年小鼠大脑的所有区域中都发现了锰元素的显着差异。