Bioinorganic chemical studies on metal superoxide dismutases and their behavior in brain by the use of multielemental analysis.

利用多元素分析对金属超氧化物歧化酶及其在大脑中的行为进行生物无机化学研究。

• 批准号: 10640539
• 负责人: AMANO Ryohei
• 金额: $ 1.98万
• 依托单位: KANAZAWA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10640539/
• 关键词: Multitracer experiment; neutron activation analysis; bio-trace element; regional brain uptake; regional brain concentration; brain fuction; age effect; brain development; mouse experiment; 生体徴量元素; 局所脳徴量元素; 脳; SOD; マルチトレーサ法; 中性子放射化分析法; Mn(マン

This study was undertaken in order to acquire better understanding of the biobehavior metal superoxide dismutases by the techniques of multitracer and instrumental neutron activation analysis. The followings were found.1) The radioactive multitracer technique was applied to a study on the uptake behavior of trace elements in normal C57BL/6N mice. Comparative uptake behavior of ^<46>Sc, ^<54>Mn, ^<59>Fe, ^<58>Co, ^<65>Zn, ^<75>Se, ^<83>Rb and ^<88>Zr tracers was examined among 11 organs (brain, cardiac muscle, lung, liver, spleen, pancreas, kidneys, bone, muscle, eyeballs and testes) and blood, and evaluated in terms of the "tissue uptake rate (the radioactivity percentage of injected dose per gram of tissue, %dose/g)". The multitracer technique revealed reliable data demonstrating characteristic uptake of the 8 trace elements, Sc, Mn, Fe, Co, Zn, Se, Rb and Zn by the brain and other organs, as well as the distinctive feature of the accumulation and retention of each element in the brai … More n. According to these results, it is evident that the metal ions examined in this work are easily transported into the brain, in addition, the high retention of Se, Rb, Mn and Zn in the brain during a fairly long time suggests active involvement of these elements in the brain function.2) Brain regional uptakes for ^<46>Sc, ^<54>Mn, ^<58>Co, ^<65>Zn, ^<75>Se, and ^<83>Rb in mice were examined using multitracer under deficient and excessive states of an essential trace elements, manganese, zinc or selenium. Zn-deficient and excessive states, and Se-deficient and excessive states influence the brain regional uptake behaviors of other trace elements except respective elements. On the other hand, in the mouse brain under Mn-deficient and excessive states, few interrelation between Mn and other elements was found from 0.4ppm to 300.4ppm. Multitracer technique was found to be very useful to screen the element-element interrelation in brain.3) Concentrations and uptake behavior of manganese (Mn), zinc (Zn) and selenium (Se) in mouse brain were studied by means of multitracer technique, neutron activation analysis and autoradiography. Comparative concentrations of Mn, Zn and Se and tracer uptake behavior of ^<54>Mn, ^<65>Zn and ^<75>Se were examined in the brain of 1-, 4-, 8-, 21- and 56-day-old mice, and evaluated in terms of brain concentration (part per million, ppm) and brain uptake rate (the radioactivity percentage of injected dose per gram of brain, %dose/g), respectively. As a result, the brain concentrations of Mn was increasing with growth, although those of Se and Zn were hardly changing. On the other hand, the uptakes of the 3 tracers by the brain of 1-day-old mice were much higher than those of other elder ones. Using radioactive ^<54>Mn as a single tracer, autoradiography was examined to known the Mn uptake regional distribution in the brain of 1-, 8- and 21-day-old mice, and observed higher regional uptake of Mn by the cerebral cortex, hippocampus, thalamus and hypothalamus in the young brain. Less

为了更好地了解金属超氧化物歧化酶的生物学行为，采用多种示踪剂和仪器中子活化分析技术进行了这项研究。结果表明：1）应用放射性多示踪技术研究了正常C57 BL/6 N小鼠对微量元素的摄取行为。在<46><54><59><58><65><75><83><88>11个器官（脑、心肌、肺、肝、脾、胰腺、肾、骨、肌肉、眼球和睾丸）和血液中检查了对Sc、Mn、Fe、Co、Zn、Se、Rb和Zr示踪剂的比较摄取行为，并根据“组织摄取率（每克组织注射剂量的放射性百分比，%剂量/g）"进行评价。多种示踪剂技术显示了可靠的数据，显示了脑和其他器官对Sc、Mn、Fe、Co、Zn、Se、Rb和Zn 8种微量元素的特征性摄取，以及每种元素在脑中积累和保留的独特特征。 ...更多信息 n.结果表明，本工作中所检测的金属离子很容易被转运到脑内，而且Se、Rb、Mn和Zn在脑内的高滞留时间较长，表明这些元素在脑功能中起着积极的作用。2）<46><54><58><65><75><83>用多种示踪剂研究了小鼠在缺乏和过量必需微量元素锰、锌或硒的情况下，脑局部对~（13）Sc、~（13）Mn、~（13）Co、~（13）Zn、~（13）Se和~（13）Rb的摄取。锌缺乏和过量状态、硒缺乏和过量状态影响除个别元素外的其它微量元素的脑区域摄取行为。而在缺锰和过量锰状态下的小鼠脑中，锰与其它元素在0.4 ~ 300.4ppm范围内几乎没有相关性。3）应用多示踪技术、中子活化分析和放射自显影技术研究了锰（Mn）、锌（Zn）和硒（Se）在小鼠脑中的含量和摄取行为。在1、4、8、21和56日龄小鼠的脑中检查Mn、Zn和Se的比较浓度以及^<54>Mn、^<65>Zn和^<75>Se的示踪剂摄取行为，并分别根据脑浓度（百万分之一，ppm）和脑摄取率（每克脑的注射剂量的放射性百分比，%剂量/g）进行评价。结果表明，脑中锰浓度随生长而增加，而硒和锌的浓度变化不大。另一方面，1日龄小鼠脑对3种示踪剂的摄取量明显高于其他年龄组小鼠。用放射性~<54>（13）Mn作单一示踪剂，用放射自显影法研究了1、8和21日龄小鼠脑内Mn摄取的区域分布，发现幼年小鼠大脑皮层、海马、丘脑和下丘脑对Mn的摄取均较高。少

项目成果

Amano R, Oishi S, Ando A, Minami T: "Instrumental Nuetron Activation Analysis of Minor and Trace Elements in Baby, Young and Aged C57BL/ON Mice"KURRI Progress Reports 1998 Section 1. 1999. 168-168 (1999)

Amano R、Oishi S、Ando A、Minami T：“婴儿、幼年和老年 C57BL/ON 小鼠中微量元素和微量元素的仪器中子激活分析”KURRI 进度报告 1998 第 1 部分。1999.168-168 (1999)

Oishi, S., Amano, R., Ando A., Enomoto, S., Ambe, F.: "Simultaneous behavior of Sc, Mn, Fe, Co, Zn, Se, Rb and Zr trace elements in brain and other organs of C57BL/6N mice."J.Radioanal.Nucl.Chem.. vol.239(2). 411-416 (1999)

Oishi, S.、Amano, R.、Ando A.、Enomoto, S.、Ambe, F.：“Sc、Mn、Fe、Co、Zn、Se、Rb 和 Zr 微量元素在大脑和其他器官中的同时行为

Tarouda J, Amano R, Sakamoto K et al: "Biobehavior of Multitrater in the brain and other argans or 1-, 3- and 8-week-old mice"RIKEN Accelerator Progress Report. 33巻印刷中. (2000)

Tarouda J、Amano R、Sakamoto K 等人：“Multitrater 在大脑和其他摩洛哥坚果或 1、3 和 8 周龄小鼠中的生物行为”RIKEN Accelerator Progress Report 第 33 卷（2000 年）。

Amano R,Enomoto S: "Brain regional uptake of radioactive Sc, Mn, Zn, Se, Rb and Zr tracers into normal mice during aging,"J.Radioanal.Nucl.Chem.,. 247(3). 507-511 (2001)

Amano R、Enomoto S：“正常小鼠在衰老过程中大脑区域摄取放射性 Sc、Mn、Zn、Se、Rb 和 Zr 示踪剂”，J.Radioanal.Nucl.Chem.,。

Amano R,Oishi S,Ishige M. Kimura M: "Brain regional distributions of the minor and trace elements, Na, Mg, Cl, K, Mn, Zn, Pb and Br in young and aged mice."Journal of Radioanalytical and Nuclear Chemistry. 247・2. 381-384 (2001)

Amano R、Oishi S、Ishige M. Kimura M：“年轻和老年小鼠中微量元素和微量元素 Na、Mg、Cl、K、Mn、Zn、Pb 和 Br 的脑区域分布。”放射分析与核杂志化学 247・2. 381-384 (2001)