Brain regional distributions of some transient metals and their bioinorganic chemical studies by the use of multielemental analysis.

使用多元素分析研究一些瞬态金属的脑区域分布及其生物无机化学研究。

• 批准号: 14540512
• 负责人: AMANO Ryohei
• 金额: $ 1.98万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14540512/
• 关键词: Multitracer experiment; neutron activation analysis; ICP-MS analysis; bio-trace element; regional brain concentration; brain function; age effect; brain development; マルチトレーサ法; 中性子放射化分析; ICP-MS; 脳; MRI; マンガン; 鉄; 生物無機化学; 亜鉛; 銅; 生物機化学

This study was undertaken in order to acquire better understanding of the brain regional biobehavior of some transient metals and their bioinorganic chemical studies by the nuclear probe techniques of the multitracer. The followings were found. 1).Mn, Fe, Cu and Zn concentrations were determined in the brain regions of normal Wistar rats using inductively coupled plasma mass spectrometry (ICP-MS), and their maps were illustrated to compare visually the distribution of the elements at the stage of the growth process. Among new findings about their brain distribution, it is especially worth noting that high concentrations of Mn, Fe and Zn were observed in the substantia nigra compared with those in neighboring regions. 2)Using the above technique, the changes in Mn, Fe, Cu and Zn concentrations were also examined in the brain of a 6-hydroxydopamine (OHDA)-induced rat model of Parkinson's disease. The results indicated that Mn, Fe, Cu and Zn levels were increased in the 6-OHDA-lesioned substantia nigra, amygdala and putamen. A marked increase in Fe level was observed in the substantia nigra of model rats. The increase in manganese level occurred earlier than those in the other elements reaching a plateau in the 6-OHDA brain, especially in the substantia nigra. The patterns in these 4 elements may hold the key to the development of Parkinson's disease. 3)We observed the direct transport of radioactive ^<86>Rb^+ and ^<201>Tl^+ via the olfactory nerve transport following intranasal administration in mice. These results evidently indicated the axonal transport of the via the olfactory nerve pathway, from nasal cavity to the olfactory bulb, and moreover to the olfactory cortex through the synaptic junctions. The olfactory transport of the ^<86>Rb^+ and ^<201>Tl^+ should represent the behavior of K^+ in the olfactory system.

本研究的目的是利用多示踪剂核探针技术，更好地了解某些过渡金属的脑局部生物行为及其生物无机化学研究。结果发现：1）.采用电感耦合等离子体质谱（ICP-MS）测定了正常Wistar大鼠脑区的锰、铁、铜和锌浓度，并绘制了它们的分布图，直观地比较了这些元素在生长过程阶段的分布。在关于其大脑分布的新发现中，特别值得注意的是，与邻近区域相比，在黑质中观察到高浓度的Mn，Fe和Zn。2)使用上述技术，还在6-羟基多巴胺（OHDA）诱导的帕金森病大鼠模型的脑中检查Mn、Fe、Cu和Zn浓度的变化。结果表明，6-OHDA损毁后，黑质、杏仁核和壳核中Mn、Fe、Cu和Zn含量增加。模型组大鼠黑质铁含量明显升高。锰水平的增加发生早于其他元素达到高原的6-OHDA脑，特别是在黑质。这4种元素的模式可能是帕金森病发展的关键。3）小鼠鼻内给药后，观察到放射性~（40）<86>Rb^+和~<201>（40）Tl^+通过嗅神经转运的直接转运。这些结果表明，嗅神经元的轴突运输是通过嗅神经通路，从鼻腔到嗅球，再通过突触连接到达嗅皮质。^<86>Rb^+和^<201>Tl^+的嗅觉运输应该代表了K^+在嗅觉系统中的行为。

项目成果

Brain regional distribution of the minor and trace elements, Na, K, So, Cr, Mn, Co, Zn and Se, in mice bred under Zn-deficient, -adequate and -excessive diets

缺锌、充足和过量饮食下饲养的小鼠中微量元素和微量元素 Na、K、So、Cr、Mn、Co、Zn 和 Se 的脑区域分布

• 发表时间: 2003
• 期刊: J.Radioanal.Nucl.Chem. vol.257(2)
• 作者: Yuko Yabushita;Yousuke Kanayama;Tohru Tarohda;Takae Tuji;Koshin Washiyama;Ryohei Amano
• 通讯作者: Ryohei Amano

Multitracer screening : Brain delivery of trace elements by eight different administration method

多重示踪剂筛选：通过八种不同的给药方法向大脑输送微量元素

• 发表时间: 2005
• 期刊: BioMetals (印刷中)
• 作者: Y.Kanayama;T.Tsuji;S.Enomoto;R.Amano
• 通讯作者: R.Amano

Yabushita Y, Kanayama Y, Tarohda T, Tuji T, Washiyama K, Enomoto S, Amano R: "Brain regional distribution of the minor and trace elements, Na, K, Sc, Cr, Mn, Co, Zn and Se, in mice bred under Zn-deficient, -adequate and -excessive diets"Journal of Radioan

Yabushita Y、Kanayama Y、Tarohda T、Tuji T、Washiyama K、Enomoto S、Amano R：“小鼠中微量元素和微量元素 Na、K、Sc、Cr、Mn、Co、Zn 和 Se 的脑区域分布

Yabushita Y, Kanayama Y, Tarohda T, Enomoto S, Amano R: "Multitracer screening for in vivo element-element interrelations Part II : Uptake of ^<65>Zn, ^<46>Sc, ^<58>Co ^<54>Mn, ^<75>Se, ^<83>Rb, ^<85>Sr and ^<88>Zr by brain and other organs of mice bred u

Yabushita Y、Kanayama Y、Tarohda T、Enomoto S、Amano R：“体内元素-元素相互关系的多重示踪剂筛选第二部分：^<65>Zn、^<46>Sc、^<58>Co ^<54 的摄取

Yabushita Y, Kanayama Y, Tarohda T, Tuji T, Washiyama K, Enomoto S. Amano R: "Brain regional distribution of the minor and trace elements, Na, K, Sc, Cr, Mn, Co, Zn and Se, in mice bred under Zn-deficient, -adequate and -excessive diets"Journal of Radioan

Yabushita Y、Kanayama Y、Tarohda T、Tuji T、Washiyama K、Enomoto S. Amano R：“小鼠中微量元素和微量元素 Na、K、Sc、Cr、Mn、Co、Zn 和 Se 的脑区域分布