Studies on the unknown ninhydrin-positive substance, appearing in the developing fruits (pericarp and seeds) of tea plants (Camellia sinensis L.)

对茶树（Camellia sinensis L.）正在发育的果实（果皮和种子）中出现的未知茚三酮阳性物质的研究

基本信息

批准号：
07660082
负责人：
SUZUKI Takeo
金额：
$ 1.28万
依托单位：
Kyoto Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

An unknown ninhydrin-positive substance, especially appearing developing fruits (pericap and seeds) of tea plants, was detected, analyzed and identified as pipecolic acid by two-dimensional thin-layr chromatography (TLC) and by an HPLC system using fluoresence detector.(a) Free amino acids were separated on TLC.The unknown compound and pipecolic acid, a cyclic amino acid, on the plate reacted with nihydrin sprays, giving distinctive color (blue). They also produced a greenish-blue color with isatin.(b) Standard solution containing amino acids, theanine, and pipecolic acid, or fresh extracts from developing tea fruits (pericarp or seeds) were separated on a Shimpack Amino-Li coloumn using a linear gradient and detected by a post-column derivative method using fluoresence detector (OPA method). In this method, more than 20 free amino acids, including theanine and pipecolic acid, were detected in tea extracts. The main free amino acid was pipecolic acid (70-80%) but not theanine in fresh unripe fruit pericarp and seeds, dissapearing in mature seeds. Lysine and *-aminoadipic acid were also detected in these extracts, suggesting that lysine-pipecolic acid pathway play an important rol in fruit development and seed formation in tea plants.(c) Pipecoli acid was also detected in flush tea leaves (5-15%), indicating that pipecolic acid is regarded as one of important free amino acids in manufactured tea leaves.

未知的二氢蛋白阳性物质，尤其是出现茶植物的开发水果（Pericap和种子），通过二维薄层色谱（TLC）（TLC）（TLC）和HPLC系统使用HPLC系统检测，分析和鉴定为寄生虫酸。与Nihydrin Sprays反应，具有独特的颜色（蓝色）。（b）使用线性梯度在Shimpack Amino-Li coloumn上分离出含有氨基酸，硫氨酸和pipecolic酸的标准溶液，或者使用线性梯度在Shimpack Amino-Li Coloumn上分离出含有氨基酸，硫氨酸和pipocolic酸的标准溶液，并使用后衍生物衍生品衍生方法在Shimpack Amino-Li Coloumn上分离，并使用column衍生物衍生物使用荧光方法（使用荧光方法）。在这种方法中，在茶提取物中检测到了20多个游离氨基酸，包括硫氨酸和脂酸。主要的游离氨基酸是脂酸（70-80％），但在新鲜的未成熟果皮和种子中却不是硫氨酸，在成熟的种子中消失。在这些提取物中还检测到赖氨酸和 * - 氨基磷酸，这表明赖氨酸 - 二贝醇酸途径在茶厂的果实发育和种子形成中起重要的ROL。（c）在冲洗茶叶（5-15％）中还检测到果脂酸（5-15％），表明含脂酸是在制造茶叶中被认为是重要的免费氨基酸的脂质酸。