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Analysis of Flowering Induced by L-Pipecolic Acid in Lemna

L-哌啶酸诱导浮萍开花的分析

基本信息

批准号：
07660146
负责人：
FUJIOKA Shozo
金额：
$ 1.41万
依托单位：
The Institute of Physical and Chemical Research (RIKEN)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660146/
关键词：
Lemna flowering flower induction L-pipecolic acid lysine gene expression protein リジン代謝

项目摘要

Previosly L-Pipecolic acid was isolated as a flower-inducing substance from Lemna extracts. A subsequent study demonstrated that L-pipecolic acid is effective in inducing flowering of many strains of Lemna. The mechanism by which L-pipecolic acid induces flowering in Lemna remains, however, to be determined. In order to clarify the flowering mechanism induced by L-pipecolic acid in Lemna, biosynthesis and metabolism of L-pipecolic acid was investigated in relation to flowering. In addition, analysis of gene and protein specifically induced by L-pipecolic acid was carried out.Lysine was found to have flower-inducing activity in Lemna, and the effect of lysine on flowering was found to be enhanced in the presence of lysine catabolite, alpha-aminoadipic acid. These results suggested that the flowering of Lemna might be induced by L-pipecolic acid, derived from exogenously applied lysine. Amino-acid analysis indicated that the level of L-pipecolic acid increased several fold by exogenous application of lysine to the medium. In addition, the conversion of lysine to L-pipecolic acid was demonstrated definitively by metabolic study using deuterium labeled lysine. Thus, the present study indicates that conversion of lysine to L-pipecolic acid induces flowering of Lemna. L-Pipecolic acid can induce flowering of Lemna by the addition of this compound to the medium. Using this system, gene and protein induced by L-pipecolic acid were examined. Treatment of L-pipecolic acid tended to increase total RNA,however, no significant change of gene expression was found. Furthermore, no specific protein induced by L-pipecolic acid was found.

l -胡椒果酸是一种从柠檬草提取物中分离得到的致花物质。随后的研究表明，l -胡椒果酸对许多柠檬属植物的开花都是有效的。然而，L-pipecolic酸诱导Lemna开花的机制仍有待确定。为了阐明l -胡椒果酸诱导柠檬花开花的机制，研究了l -胡椒果酸的生物合成和代谢与柠檬花开花的关系。此外，还对l -胡椒果酸特异性诱导的基因和蛋白进行了分析。研究发现，赖氨酸在Lemna中具有诱导花的活性，并且在赖氨酸分解物α -氨基己二酸的存在下，赖氨酸对开花的作用增强。这些结果表明，外源赖氨酸衍生的l -胡椒果酸可能诱导了柠檬花的开花。氨基酸分析表明，外源赖氨酸使l -胡椒果酸水平增加了数倍。此外，用氘标记赖氨酸的代谢研究明确地证明了赖氨酸向l -管果酸的转化。因此，本研究表明赖氨酸转化为l -胡椒果酸诱导了Lemna的开花。L-Pipecolic acid可以通过在培养基中添加该化合物诱导Lemna开花。利用该系统对l -管果酸诱导的基因和蛋白进行了检测。l -胡椒果酸处理有增加总RNA的趋势，但基因表达无明显变化。此外，没有发现l -胡椒果酸诱导的特异性蛋白。