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Development of evaluation for human exposure to highly toxic dioxin analogues using hair as an indicator sample

以头发为指标样品开展人体接触剧毒二恶英类似物的评估

基本信息

批准号：
07670468
负责人：
MIYATA Hideaki
金额：
$ 1.41万
依托单位：
Setsunan University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670468/
关键词：
Dioxin analogues PCDD PCDF Coplanar PCB Hair Pollution Incineration facility Analysis PCDF ユプラナーPCB

项目摘要

All dioxin analogues composing of polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and coplanar PCBs (Co-PCBs) are very toxic. Even at environmental pollution level, it is revealed that they give a hormonal damage in women, and pointed out that they will occur immunosuppression and genital function damage in human. Therefore, in recent years, U.S.A., Canada and Europe countries have actively took measures to decrease the environmental pollution level.In this study, a new method with a combination of isotope dilution method was established for dioxin analogues in human hair. This method, which was composed of washing and cutting of hair, alkaline decomposition, hexane extraction, multi-layr silica gel column chromatography, alumina column chromatography and analysis by gas chromatograph/high resolution mass spectrometer, enable us to analyze dioxin analogues at an extra-trace level of less than pg/g with a good reproducibility.Using this method, hair samples from 80 control persons and from 60 workers in municipal solid waste (MSW) incineration facilities were analyzed. Taking the results of analytical data and questionnaire about age, diet habit, work, smoking, drinking etc., the hair was confirmed to be a good indicator sample for atmospheric burden of dioxin analogues. The worker was toxicologically exposed to dioxin analogues a few times greater than did the control, indicating the indoor environment of the facility to be more heavily polluted in comparison to the general environment. In addition, there was a difference in pollution degree among working places of the facility.From above results, it is concluded that this hair monitoring technique is a good means for the total evaluation of human exposure to dioxin analogues via many media.

所有由多氯二苯并对二恶英(PCDDs)、多氯二苯并呋喃(PCDf)和共面多氯联苯(Co-PCbs)组成的二恶英类似物都具有很强的毒性。即使在环境污染水平上，也有研究表明，它们会对女性造成荷尔蒙损伤，并指出它们会对人类产生免疫抑制和生殖功能损害。因此，近年来，美国、加拿大和欧洲国家积极采取措施降低环境污染水平。本研究建立了一种结合同位素稀释法测定头发中二恶英类似物的新方法。该方法包括头发的清洗和切割、碱解、正己烷提取、多层硅胶柱层析、氧化铝柱层析和气相色谱/高分辨质谱仪分析，使我们能够在超痕量水平上分析二恶英类似物，重现性好。用该方法分析了城市垃圾焚烧设施中80名对照人员和60名工人的头发样品。结合年龄、饮食习惯、工作、吸烟、饮酒等分析数据和问卷调查结果，证实头发是大气中二恶英类似物负荷的良好指示性样本。这名工人暴露在二恶英类似物中的毒理作用比对照组大几倍，这表明与一般环境相比，该设施的室内环境污染更严重。此外，设施不同工作场所的污染程度也存在差异。从以上结果来看，该毛发监测技术是一种通过多种媒介全面评估人体接触二恶英类似物的良好手段。