Exploration of the DNA-recognition site for anti-DNA antibody inducing DNA-binding protein nucleobindin

点击翻译按钮获取中文摘要

We have achieved the following progresses in the studies on physiological activity and fine characteristics of nucleobindin.1. Administration of recombinant nucleobindin induced production of autoantibodies such as anti-DNA antibody even in normal BALB/c mice that have no genetical background of autoimmune disorders.2. Enhanced expression of nucleobindin mRNA was observed in the lymphoid organs of lupus-prone strains of mice such as MRL/lpr, C3H/gld, and male BXSB.3. Enhanced synthesis of nucleobindin in lupus-prone mice was demonstrated also in the protein level by immunohistochemical staining using an antibody produced against neclebindin peptide.4. A monoclonal antibody to human nucleobindin successfully immunoprecipitated native nucleobindin in the nuclear extract of human malignant lymphoma cell line Molt-4.5. The oligonucleotides which showed high binding affinity to human nucleobindin were selected from synthesized oligonucleotides containig 26-base-long random sequences. Some motif were repeatedly found in the selected oligonucleotides. Furthermore, such motif as CCACCACAATA and GTGTGGA were found in the up-stream region of IL-2 gene. It remains elusive how nucleobindin is involved in regulation of gene transcription.