Specific elimination of anti-DNA antibody-producing cells in systemic lupus erythematosus.

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The specific manipulation of anti-DNA antibody production is one of the prefered treatment of systemic lupus erythematosus (SLE). For this purpose, we induced DNE-specific tolerance in vitro using DNA-Dglutamic acid-D-lysine(DNA-D-GL).The addition of the tolerogen reduced markedly the in vitro production of spontaneously occuring anti-ds and/or anti-ss DNA antibodies by SLE lymphocytes. The unresponsiveness was DNA-specific.We also used monoclonal anti-idiotypic (Id) antibodies which were conjugated with neocarzinostatin (NCS), a cytotoxic agent. Human B cell clones, which produce anti-DNA autoantibodies, were killed by by in vitro treatment with anti-Id-NCS conjugates, while clones expressing an Id with irrelevant specificity were unaffected. Anti-Id-NCS also specifically suppressed anti-DNA autoantibodyproductin in vitro by human lymphocytes.This approach will have a significant advantages in aborting clones, and will result in a potent therapeutic treatment for SLE.