A STUDY OF GENETHERAPY AGAINST ORAL CANCER TARGETTED TO THE GENE OF AUTOCRINE GROWTH FACTOR

成纤维细胞生长因子-1（FGF-1）和FGF-2是广谱有丝分裂原。本文应用免疫组织化学方法检测了涎腺恶性肿瘤和正常涎腺组织中FGF-1、FGF-2及其受体FGF-1（FGFR-1）的表达。在7例腺样囊性癌中，导管样细胞和修饰肌上皮细胞FGF-1、FGF-2和FGFR-1均呈明显阳性。5例粘液表皮癌（MC）的表皮样细胞、粘液细胞和中间细胞均表达FGF-1、FGF-2和FGFR-1。在这些恶性涎腺肿瘤中，FGFR-1表达增加与FGF-2和FGF-2免疫反应性的强度相关。与恶性涎腺肿瘤相比，8例正常涎腺组织中FGF-1、FGF-2和FGFR-1均为阴性，4例FGF及其受体呈弱阳性，提示恶性涎腺肿瘤中FGF-1、FGF-2和GFR-1的表达高于正常涎腺组织。提示这些生长因子可能在促进人涎腺肿瘤进展中起重要作用。

Fibroblast growth factor-1 (FGF-1) and FGF-2 are broad spectrum mitogens. Here, th expression of FGF-1, FGF-2 and their receptor, FGF receptor-1 (FGFR-1) was examined i malignant salivary gland tumors and normal salivary glands using immunohistochemical methods. I seven cases of adenoid cystic carcinoma (ACC), both duct-like cells and modified myoepithelial cell were apparently immunopositive for FGF-1, FGF-2 and FGFR-1. In five cases of mucoepidermoi carcinoma (MC), all three types of tumor cells including epidermoid cells, mucous cells an intermediate cells expressed immunoreactive FGF-1, FGF-2 and FGFR-1. In these malignant salivar gland tumors, increased expression of FGFR-1 correlated with the intensity of both FGF- and FGF-2 mmunoreactivity. In contrast to malignant salivary gland tumors, eight cases of normal salivary glan howed negative immunostaining for FGF-1, FGF-2 and FGFR-1 while four cases were wea immunoreactivity for FGF and its receptor.These results demonstrated that malignant salivary gland tumors overexpress FGF-1, FGF-2 an GFR-1 compared to normal salivary glands, suggesting that these growth factors may play a mportant role in facilitating its neoplastic progression in human salivary gland.