A STUDY OF GENETHERAPY AGAINST ORAL CANCER TARGETTED TO THE GENE OF AUTOCRINE GROWTH FACTOR

针对自分泌生长因子基因的口腔癌基因治疗研究

• 批准号: 07807184
• 负责人: OKAMOTO Tetsuji
• 金额: $ 1.34万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07807184/
• 关键词: FGF-1; FGF-2; FGF-7; KGF; serum-free culture; squawous cell cariuown; salivary gland tuwor; avti seuse oligo; dominant-negative reoptor; 自己増殖因子

Fibroblast growth factor-1 (FGF-1) and FGF-2 are broad spectrum mitogens. Here, th expression of FGF-1, FGF-2 and their receptor, FGF receptor-1 (FGFR-1) was examined i malignant salivary gland tumors and normal salivary glands using immunohistochemical methods. I seven cases of adenoid cystic carcinoma (ACC), both duct-like cells and modified myoepithelial cell were apparently immunopositive for FGF-1, FGF-2 and FGFR-1. In five cases of mucoepidermoi carcinoma (MC), all three types of tumor cells including epidermoid cells, mucous cells an intermediate cells expressed immunoreactive FGF-1, FGF-2 and FGFR-1. In these malignant salivar gland tumors, increased expression of FGFR-1 correlated with the intensity of both FGF- and FGF-2 mmunoreactivity. In contrast to malignant salivary gland tumors, eight cases of normal salivary glan howed negative immunostaining for FGF-1, FGF-2 and FGFR-1 while four cases were wea immunoreactivity for FGF and its receptor.These results demonstrated that malignant salivary gland tumors overexpress FGF-1, FGF-2 an GFR-1 compared to normal salivary glands, suggesting that these growth factors may play a mportant role in facilitating its neoplastic progression in human salivary gland.

成纤维细胞生长因子-1(成纤维细胞生长因子-1)和成纤维细胞生长因子-2是广谱有丝分裂原。本研究采用免疫组织化学方法检测了恶性涎腺肿瘤和正常涎腺组织中成纤维细胞生长因子-1、成纤维细胞生长因子-2及其受体FGFR-1的表达。在7例腺样囊性癌(ACC)中，导管样细胞和改良肌上皮细胞均呈阳性表达。在5例粘液表皮样癌(MC)中，表皮样细胞、粘液细胞和中间细胞3种类型的肿瘤细胞均表达免疫反应阳性的FGF1、FGFR2和FGFR 1。在这些恶性涎腺肿瘤中，FGFR-1的表达增加与成纤维细胞生长因子-2和成纤维细胞生长因子-2的免疫反应强度有关。与涎腺恶性肿瘤相比，8例正常涎腺呈阴性表达，4例呈阳性表达，提示涎腺恶性肿瘤较正常涎腺过度表达，提示这些生长因子可能在涎腺肿瘤的发生发展中起重要作用。

项目成果

Okamoto, T., Myoken, Y., Yabumoto, M., Osaki, T., Fujita, Y., Whitney, R.G., Kan, M., Crabb, J.W., Sato, G.H., Kato, Y., Takada, K.and Sato, J.D.: "Androgen-dependent expression of fibroblast growth factor-1 in submaxillary gland of mouse." Biochem.Biophy

冈本，T.，Myoken，Y.，薮本，M.，大崎，T.，藤田，Y.，惠特尼，R.G.，坎，M.，克拉布，J.W.，佐藤，G.H.，加藤，Y.，高田，K

岡本哲治,明見能成: "細胞成長因子 partIII,Vascular endothelial growth factor" 朝倉書店、日本組織培養学会編(印刷中), (1996)

冈本哲二、麻美吉成：《细胞生长因子第III部分，血管内皮生长因子》朝仓书店，日本组织培养学会编（正在出版），（1996年）

Myoken,Y: "Immunohistochemical study of overexpression of FGF-1 FGF-2 and FGF receptor-1 in human malignorit Salivary fumor" J.Pathology. 178. 429-436 (1996)

Myoken，Y：“人恶性唾液腺肿瘤中 FGF-1、FGF-2 和 FGF 受体 1 过度表达的免疫组织化学研究”J.病理学。

Okamoto,T: "Differential expression of FGFR1-FGFR4 genes is associcuted with maligncont progression in huncosr salivary gland tumor" Oral Oncology. 2. 316-319 (1995)

Okamoto,T：“FGFR1-FGFR4 基因的差异表达与 huncosr 唾液腺肿瘤的恶性进展相关”口腔肿瘤学。

Myoken,Y: "Expression of FGF-1 and FGF-2 and FGFR-1 in a human salivary gland adenocarcinonra cell line" Int.J.Cancer. 65. 650-657 (1996)

Myoken，Y：“FGF-1、FGF-2 和 FGFR-1 在人唾液腺腺癌细胞系中的表达”Int.J.Cancer。