Studies on mechanisms of pathogenesis of internal derangement of temporomandibular joints by cytokine gene analysis.

通过细胞因子基因分析研究颞下颌关节内部紊乱的发病机制。

• 批准号: 07838005
• 负责人: TAKAHASHI Tetsu
• 金额: $ 1.66万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07838005/
• 关键词: cytokine; internal derangement; synovial fluid; nitric oxide; inflammation; temporomandibular joint; interleukin-1beta; temporomandibular disorders; 滑液解析; 炎症性サイトカイン; Nitric Oxide; 変形性顎関節症; Interleukin 1β; 遺伝子; 滑膜; PCR

In this study, experiments were initially scheduled to detect gene expression of cytokines in synovial-tissue specimens taken from patients with internal derangement of the temporomandibular joint during arthroscopic surgery. However, we could have not confirm the experiment system by arthroscopic biopsy of these pathologic tissue, mainly because of the lack of surgical specimens and technical problems. Then we shifted the focus of this study to the detection of proinflammatory cytokines and nitric oxide in synovial fluids taken from patients with internal derangement and osteoarthritis of the temporomandibular joints. The results are as follow :1.Although none of proinflammatory cytokines were detectable in the synovial fluid in the control group, measurable levels of cytokines including interleukin (IL)-1beta, IL-6, IL-8, TNFalpha, Interferon gamma.2.There was a strong correlation between the detection of IL-1beta and pain in the joint area.3.Elevated lefvels of nitric oxide were demonstrated in synovial fluid from patients with temporomandibular disorders.4.There was a strong correlation between the levels of IL-1beta and nitric oxide in SF from the patients.These findings were published in several journals including Oral Surg Oral med Oral Pathol Oral Radiol Endod 1996 ; 82 : 505-509 and Oral Surg Oral med Oral Pathol Oral Radiol Endod 1998 ; 885 : 135-141.These data clearly suggested that these proinflammatory cytokines and nitric oxided are involved in the pathogenesis of synovitis and degenerative changes of the cartilaginous tissue and bone of the temporomandibular joints.This study seems to shed a light of the mechanisms of internal derangement by the way of biochemical manner.

在这项研究中，实验最初计划检测滑膜组织标本中细胞因子的基因表达，该标本取自关节镜手术中的颞下颌关节内错乱患者。然而，由于缺乏手术标本和技术问题，我们无法通过关节镜下对这些病理组织进行活检来确定实验系统。然后，我们将研究的重点转移到从关节内紊乱和骨关节炎患者的滑液中检测促炎细胞因子和一氧化氮。结果如下：1.对照组滑液中未检测到致炎细胞因子，但可检测到IL-1β、IL-6、IL-8、肿瘤坏死因子α、干扰素-γ等细胞因子的水平。2.IL-1β的检测与关节区疼痛密切相关。3.颞下颌关节紊乱病患者滑液中一氧化氮水平升高。4.患者关节滑液中IL-1β水平与一氧化氮水平呈正相关。这一结果发表在《外科杂志》、《病理学》、《放射学》等期刊上；82：505-509和《口腔外科》《口腔病理》《口腔放射学》1998；885：135-141。这些资料清楚地表明，这些促炎细胞因子和一氧化氮参与了滑膜炎的发病以及关节软骨组织和骨的退行性改变。本研究从生化的角度揭示了关节内紊乱的机制。

项目成果

Takahashi T: "Proinfolmmatory cytokins in synoual Fluids from tempormmandihlaf Jort:its clihaical sognificanece" J.Jpn.Soc.TMJ. 9. 218-219 (1997)

Takahashi T：“tempormmandihlaf Jort 滑液中的原信息细胞因子：其临床意义”J.Jpn.Soc.TMJ。

Takahashi T, et al.: "Nitric oxide production in the lescrs of temporoomandiblar disorders and geuderdiffeences in nitricoxide production letters to the editor" Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 84. 330-331 (1997)

Takahashi T 等人：“致编辑的颞下颌疾病的眼睑中一氧化氮的产生和一氧化氮产生的 geudiffeences 的信” Oral Surg Oral Med Oral Pathol Oral Radiol Endod。

TAKAHASHI T: "Elevated levels of nitric oxidein synovial fluid from patients with temporomandibular disorders" Oral Surg Oral Med Oral Pathol. 82. 505-509 (1996)

TAKAHASHI T：“颞下颌疾病患者滑液中一氧化氮水平升高”口腔外科口腔医学口腔病理学。

Takahashi T, et al.: "Elevated levels of nitric oxide in symovial fuild from patisuts with tew poromacdibular Digorders" Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 82. 505-509 (1996)

Takahashi T 等人：“患有tew poromacdibular Digorders 的patisuts 中一氧化氮水平升高” Oral Surg Oral Med Oral Pathol Oral Radiol Endod。

TAKAHASHI T: "Proinflammarory cytokines detectable in synovial fhid from patients with temporomaudibulan disorders" Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 85. 135-141 (1998)

TAKAHASHI T：“颞下颌关节疾病患者的滑液中可检测到促炎细胞因子”Oral Surg Oral Med Oral Pathol Oral Radiol Endod。