Biochemical analysis for the effect of mechanical stress on temporomandibular disorders

机械应力对颞下颌疾病影响的生化分析

• 批准号: 10671863
• 负责人: TAKAHASHI Tetsu
• 金额: $ 2.24万
• 依托单位: Kyusyu Dental College (2000-2001)Akita University (1998-1999)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671863/
• 关键词: temporomandibular disorders; internal derangement; mechanical stress; synovial fluid; synovial fluid analysis; cytokine; nitric oxide; hyaluronic acid; フリーラジカル; 滑膜炎; 炎症性メディエーター; iNOS; IL-1β; 顎関節鏡; inducible nitric oxide synthase; 軟骨変性

The purpose of this study is to biochemically analyze the levels of cytokines and nitric oxides in the synovial fluid from patients with patients with internal derangement of the temporomandibular joint, and to investigate the relationship between the mechanical stress such as bruxism and the levels of proinflammatory mediators in the synovial fluid. It was extremely difficult in synovial fluid sampling from the patients with internal derangement and bruxism. Therefore, we could not directly compare the levels of proinflammatory mediators and the presence of mechanical stress. However, We demonstrated that the synovial levels of protein were correlated with joint effusion. In addition, inducible nitric oxide synthase was expressed in the synovial lining cell layers in the synovium of the patients with temporomandibular diseases, not from the control patients. Furthermore, the molecular weight of the hyaluronic acid in the synovial fluid was decreased compared with that of the controls. These biochemical data strongly suggested that mechanical stress in involved in the pathogenesis of the temporomandibular joint diseases such as internal derangement.

本研究旨在生化分析颞下颌关节内部紊乱患者滑液中细胞因子和一氧化氮的水平，并探讨磨牙等机械应力与滑液中促炎介质水平的关系。对内部紊乱和磨牙患者的滑液取样是非常困难的。因此，我们不能直接比较促炎介质的水平和机械应力的存在。然而，我们证明了滑膜蛋白水平与关节积液相关。此外，诱导型一氧化氮合酶在颞下颌疾病患者的滑膜衬里细胞层中表达，而在对照组中没有表达。此外，与对照组相比，滑液中透明质酸的分子量降低。这些生化数据有力地提示机械应力参与了颞下颌关节内部紊乱等疾病的发病机制。

项目成果

高橋哲,永井宏和 他: "Joint effusionを有する顎関節症患者における滑液中の蛋白濃度の上昇"東北大学歯学雑誌. 18. 149-158 (1999)

Tetsu Takahashi、Hirokazu Nagai 等人：“伴有关节积液的颞下颌关节疾病患者滑液中蛋白质浓度增加”，东北大学牙科杂志 18. 149-158 (1999)。

高橋哲、永井宏和 他: "Joint effusiouを有する顎関節症患者によりける滑液中の蛋白濃皮の上昇"東北大学歯学雑誌. 18. 149-158 (1999)

Tetsu Takahashi、Hirokazu Nagai 等人：“颞下颌关节疾病患者滑液中的蛋白质密度增加”东北大学牙科杂志 18. 149-158 (1999)。

Takahashi T 他 3名: "Synovial osteochondromatosis accompanied with ossified articular disk in the temporomandibular joint : a case report"J Oral Pathol Med. (in press). (2003)

Takahashi T 和其他 3 人：“滑膜骨软骨瘤病伴有颞下颌关节骨化：病例报告”J Oral Pathol Med（出版中）。

高橋哲,大谷真紀 他: "顎関節鏡視診断と顎関節滑液中の炎症性メディエーターの比較検討"関節鏡. 25. 127-131 (2000)

Satoshi Takahashi、Maki Otani 等：“颞下颌关节镜诊断和颞下颌关节滑液中炎症介质的比较研究”关节镜检查。

Takahashi T: "Biochemital changes associted with pathology of the tempromandibular joint : nitric oxide production and inducible nitric oxide synthase expression in temporomandionbular joint disorder"Bulletin of Kanagawa Doutal College. 29(印刷中). (2001)

Takahashi T：“与颞下颌关节病理学相关的生化变化：颞下颌关节疾病中一氧化氮的产生和诱导型一氧化氮合酶的表达”，神奈川道塔学院通报29（出版中）。