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Involvement of Enteric Nervous System in the Regulation of Gastrointestinal Motility

肠神经系统参与胃肠动力的调节

基本信息

批准号：
07671384
负责人：
FUJIMURA Masaki
金额：
$ 1.34万
依托单位：
Shiga University of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671384/
关键词：
peristalsis ENS ex vivo isolated vascularly perfused intestinal model serotonin depleted model p-chlorophenylalanine (PCPA)serotonin serotonin receptor serotonin receptor antagonist

项目摘要

Serotonin (5-HT)-containing neurons in the gastrointestinal (GI) tract are thought to function as interneurons in the enteric nervous system. However, the details of the action of 5-HT on GI function has not been clarified. The purpose of the present study was to clarify the functional role of 5-HT on the motor activity of the intestine by using an ex vivo model of isolated, vasculary perfused rat duodenum. We especially saught to investigate the involvement of 5-HT3 and 5-HT4 receptors on peristaltic movement of the bowel.Method : The duodenal segment between pylorus and ligament of Treiz was vascularly perfused with O2 saturated Krebs' bicarbonate solution containing 3% dextran and 0.2% BSA.The motor activity was measured by a manometric method as follows : two manometric catheters were inserted through the pylorus and the tips were placed at 3 and 8 cm distant from the pylorus. Motor activity of duodenum in p-chlorophenylalanine (PCPA) treated animala, 5-HT depleted model, was exami … More ned in ex vivo isolated vascularly perfused preparation. Different 5HT receptor antagonists such as methysergide (5-HT1), ketanserin (5-HT2), granisetron and azasetron (5-HT3) and SB204070 (5-HT4) were introduced into the perfusate. The effect of these drugs on motor index (MI), %MI (% of control value) and frequency were compared with controls.Result : In normal conditions the motor activity was observed to consiste of a period of quiescence followed by contractions. Such contractile activity was defined as clusters of phasic contractions (COC). The frequency of CPC was 0.991+0.052/min, the duration was 30.0+2.0sec and the MI was 655+89cm H20/sec. Both mucosal and musclar layr 5-HT content were significantly lower than that in controls. Neither 5-HT1 (1 mM methysergide) nor 5-HT2 (10 mM ketanserin) changed the pattern of CPC.5-HT3 (0.1 nM granisetron) decreased % MI and the frequency of CPC.5-HT4 (0.1 nM SB204070) did not affect the frequency of CPC,however it decreased % MI of CPC.Conclusion : Endogenous 5-HT may affect the motor activity in the rat duodenum and this action seems to be mediated by 5-HT3 and 5-HT4 receptpors. Less

胃肠道中的含5-羟色胺（5-HT）的神经元被认为是肠神经系统中的中间神经元。然而，5-HT对GI功能的作用的细节尚未阐明。本研究的目的是阐明5-羟色胺的功能作用的运动活动的肠道，通过使用离体模型，血管灌注大鼠十二指肠。方法：用含3%右旋糖酐和0.2%BSA的饱和Krebs碳酸氢盐溶液对十二指肠幽门和Treiz韧带之间的肠段进行血管灌流，用测压法测定肠蠕动活动。通过幽门插入两个测压导管，并将尖端放置在距离幽门3和8 cm处。检查了对氯苯丙氨酸（PCPA）处理的动物（5-HT耗尽模型）的十二指肠运动活动 ...更多信息在离体分离的血管灌注制剂中进行。将不同的5 HT受体拮抗剂如甲基麦角酰胺（5-HT 1）、酮色林（5-HT 2）、格拉司琼和阿扎司琼（5-HT 3）和SB 204070（5-HT 4）引入灌注液中。结果：正常情况下，运动活动由静止期和收缩期组成，运动指数（MI）、%MI（对照值的%）和频率的变化与对照组比较。这种收缩活动被定义为阶段性收缩簇（COC）。CPC频率为0.991 ± 0.052次/分，持续时间为30.0 ± 2.0秒，MI为655 ± 89 cm H2O/秒。粘膜和肌层5-HT含量均显著低于对照组。5-HT1（1 mM麦角新碱）去甲5-HT 2（10 mM酮色林）改变了CPC的模式。（0.1 nM格拉司琼）降低% MI和CPC频率。（0.1 nM SB 204070）不影响CPC的频率，但其降低CPC的% MI。内源性5-HT可影响大鼠十二指肠的运动，其作用可能是通过5-HT_3和5-HT_4受体介导的。少