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Involvement of Enteric Nervous System in the Regulation of Gastrointestinal Motility

肠神经系统参与胃肠动力的调节

基本信息

批准号：
07671384
负责人：
FUJIMURA Masaki
金额：
$ 1.34万
依托单位：
Shiga University of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671384/
关键词：
peristalsis ENS ex vivo isolated vascularly perfused intestinal model serotonin depleted model p-chlorophenylalanine (PCPA)serotonin serotonin receptor serotonin receptor antagonist

项目摘要

Serotonin (5-HT)-containing neurons in the gastrointestinal (GI) tract are thought to function as interneurons in the enteric nervous system. However, the details of the action of 5-HT on GI function has not been clarified. The purpose of the present study was to clarify the functional role of 5-HT on the motor activity of the intestine by using an ex vivo model of isolated, vasculary perfused rat duodenum. We especially saught to investigate the involvement of 5-HT3 and 5-HT4 receptors on peristaltic movement of the bowel.Method : The duodenal segment between pylorus and ligament of Treiz was vascularly perfused with O2 saturated Krebs' bicarbonate solution containing 3% dextran and 0.2% BSA.The motor activity was measured by a manometric method as follows : two manometric catheters were inserted through the pylorus and the tips were placed at 3 and 8 cm distant from the pylorus. Motor activity of duodenum in p-chlorophenylalanine (PCPA) treated animala, 5-HT depleted model, was exami … More ned in ex vivo isolated vascularly perfused preparation. Different 5HT receptor antagonists such as methysergide (5-HT1), ketanserin (5-HT2), granisetron and azasetron (5-HT3) and SB204070 (5-HT4) were introduced into the perfusate. The effect of these drugs on motor index (MI), %MI (% of control value) and frequency were compared with controls.Result : In normal conditions the motor activity was observed to consiste of a period of quiescence followed by contractions. Such contractile activity was defined as clusters of phasic contractions (COC). The frequency of CPC was 0.991+0.052/min, the duration was 30.0+2.0sec and the MI was 655+89cm H20/sec. Both mucosal and musclar layr 5-HT content were significantly lower than that in controls. Neither 5-HT1 (1 mM methysergide) nor 5-HT2 (10 mM ketanserin) changed the pattern of CPC.5-HT3 (0.1 nM granisetron) decreased % MI and the frequency of CPC.5-HT4 (0.1 nM SB204070) did not affect the frequency of CPC,however it decreased % MI of CPC.Conclusion : Endogenous 5-HT may affect the motor activity in the rat duodenum and this action seems to be mediated by 5-HT3 and 5-HT4 receptpors. Less

胃肠道 (GI) 中含有血清素 (5-HT) 的神经元被认为在肠神经系统中充当中间神经元。然而，5-HT对胃肠道功能的作用细节尚未明确。本研究的目的是通过使用离体、血管灌注的大鼠十二指肠离体模型来阐明 5-HT 对肠道运动活动的功能作用。我们特别致力于研究 5-HT3 和 5-HT4 受体对肠道蠕动的影响。方法：用含有 3% 葡聚糖和 0.2% BSA 的 O2 饱和 Krebs' 碳酸氢盐溶液对幽门和 Treiz 韧带之间的十二指肠段进行血管灌注。通过如下测压方法测量运动活动：两次测压导管通过幽门插入，尖端放置在距幽门 3 和 8 cm 处。在离体分离的血管灌注制剂中检查对氯苯丙氨酸 (PCPA) 处理的动物（5-HT 耗尽模型）中十二指肠的运动活动。将不同的 5HT 受体拮抗剂如美西麦角 (5-HT1)、酮色林 (5-HT2)、格拉司琼和阿扎司琼 (5-HT3) 以及 SB204070 (5-HT4) 引入灌注液中。将这些药物对运动指数（MI）、％MI（对照值的％）和频率的影响与对照进行比较。结果：在正常条件下，观察到运动活动由一段静止期和随后的收缩组成。这种收缩活动被定义为阶段性收缩簇（COC）。 CPC的频率为0.991+0.052/min，持续时间为30.0+2.0秒，MI为655+89cm H2O/秒。粘膜层和肌层5-HT含量均显着低于对照组。 5-HT1 (1 mM 美西麦角) 和 5-HT2 (10 mM 酮色林) 均未改变 CPC 的模式。5-HT3 (0.1 nM 格拉司琼) 降低了 % MI 和 CPC 的频率。5-HT4 (0.1 nM SB204070) 不影响 CPC 的频率，但降低了 CPC 的 % MI。结论：内源性 5-HT可能会影响电机大鼠十二指肠中的活性，该作用似乎是由 5-HT3 和 5-HT4 受体介导的。较少的