Involvement of Enteric Nervous System in the Regulation of Gastrointestinal Motility

肠神经系统参与胃肠动力的调节

基本信息

批准号：
07671384
负责人：
FUJIMURA Masaki
金额：
$ 1.34万
依托单位：
Shiga University of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671384/
关键词：
peristalsis ENS ex vivo isolated vascularly perfused intestinal model serotonin depleted model p-chlorophenylalanine (PCPA)serotonin serotonin receptor serotonin receptor antagonist

项目摘要

Serotonin (5-HT)-containing neurons in the gastrointestinal (GI) tract are thought to function as interneurons in the enteric nervous system. However, the details of the action of 5-HT on GI function has not been clarified. The purpose of the present study was to clarify the functional role of 5-HT on the motor activity of the intestine by using an ex vivo model of isolated, vasculary perfused rat duodenum. We especially saught to investigate the involvement of 5-HT3 and 5-HT4 receptors on peristaltic movement of the bowel.Method : The duodenal segment between pylorus and ligament of Treiz was vascularly perfused with O2 saturated Krebs' bicarbonate solution containing 3% dextran and 0.2% BSA.The motor activity was measured by a manometric method as follows : two manometric catheters were inserted through the pylorus and the tips were placed at 3 and 8 cm distant from the pylorus. Motor activity of duodenum in p-chlorophenylalanine (PCPA) treated animala, 5-HT depleted model, was exami … More ned in ex vivo isolated vascularly perfused preparation. Different 5HT receptor antagonists such as methysergide (5-HT1), ketanserin (5-HT2), granisetron and azasetron (5-HT3) and SB204070 (5-HT4) were introduced into the perfusate. The effect of these drugs on motor index (MI), %MI (% of control value) and frequency were compared with controls.Result : In normal conditions the motor activity was observed to consiste of a period of quiescence followed by contractions. Such contractile activity was defined as clusters of phasic contractions (COC). The frequency of CPC was 0.991+0.052/min, the duration was 30.0+2.0sec and the MI was 655+89cm H20/sec. Both mucosal and musclar layr 5-HT content were significantly lower than that in controls. Neither 5-HT1 (1 mM methysergide) nor 5-HT2 (10 mM ketanserin) changed the pattern of CPC.5-HT3 (0.1 nM granisetron) decreased % MI and the frequency of CPC.5-HT4 (0.1 nM SB204070) did not affect the frequency of CPC,however it decreased % MI of CPC.Conclusion : Endogenous 5-HT may affect the motor activity in the rat duodenum and this action seems to be mediated by 5-HT3 and 5-HT4 receptpors. Less

胃肠道（GI）中含有5-羟色胺（5-HT）的神经元被认为在肠神经系统中充当中间神经元。但是，尚未阐明5-HT对GI功能的作用的细节。本研究的目的是通过使用分离的，血管灌注大鼠十二指肠的体内模型来确定5-HT在肠子运动活动中的功能作用。我们特别试图研究5-HT3和5-HT4受体在肠道蠕动运动中的参与。方法：pyrorus和treiz韧带之间的十二指肠段是用O2饱和的Krebs饱和的Krebs添加剂灌注的碳酸氢盐的碳酸氢盐解决方案，其中包含3％的右旋和0.2％BSA。通过pyr插入，将尖端放置在距离皮林的3和8 cm处。十二指肠在p-氯苯乙丙氨酸（PCPA）处理的动物A耗尽模型中的运动活性是在离体分离的血管灌注制剂中进行的。引入了不同的5HT受体拮抗剂，例如甲基抗苷酸（5-HT1），Ketanserin（5-HT2），Granisetron和AzaseTron（5-HT3）和SB204070（5-HT4）。将这些药物对运动指数（MI），％MI（对照值的百分比）和频率的影响与对照组进行了比较。恢复：在正常条件下，观察到运动活性遏制了静止期，然后收缩。这种收缩活动定义为阶段收缩簇（COC）。 CPC的频率为0.991+0.052/min，持续时间为30.0+2.0秒，MI为655+89cm H20/sec。粘膜和Musclar Layr 5-HT含量均显着低于对照组的含量。 5-HT1（1毫米甲基抗苷酸）和5-HT2（10 mM酮酸）均未改变CPC.5-HT3（0.1 nm Granisetron）的模式降低％MI，CPC.5-HT4（5-HT4）的频率（0.1 nm SB204070）（0.1 nm SB204070）不会影响CPC的频率，但是CPC降低了CPC，cpc is it it it is it it is of it is is 5 ens降低了。大鼠十二指肠和该作用的运动活性似乎是由5-HT3和5-HT4受体介导的。较少的