Investigation of the metastatic mechanism by a new in vitro invasion asseay using monolayrs of vascular endothelial cells.

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Metastasis is a complex process. The interaction between endothelial cells and cancer cells is very important in this process. We established an in vitro invasion model using monolayrs of endothelial cells for investigation of this interaction. In human renal cancer cell lines, highly metastatic cell lines were more invasive than their low metastatic counterpart in our in vitro model. Our in vitro invasion assay using calf pulumonary arterial endothelial cells would be useful to evaluate protease activities and colony formation during invasion. Further examination of other sets of low and highly metastatic carcinoma cell lines is required to evaluate this in vitro invasion assay system. Now, we are producing several cell lines which will be useful for our invasion assay. In human renal cancer cell lines, we indicated the increased mRNA expressions of t-PA,u-PA and c-met by morthern blot analysis. In human colon carcinoma cell lines, we indicated the increased mRNA expressions of u-PA,MMP-2 and c-met by northern blot analysis, and we also indicated the increased expression of Siaryl Lewis^a and decreased expression of E-cadherin at cell surface by flow-cytometric analysis. These results were reported at some conferences and papors.