An analysis of mitochondrial transport mechanisms and physiological functions of mammalian molecular chaperone HSP60

哺乳动物分子伴侣HSP60线粒体转运机制及生理功能分析

• 批准号: 12670105
• 负责人: ITOH Hideaki
• 金额: $ 2.18万
• 依托单位: AkitaUniversity
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670105/
• 关键词: Molecular chaperone; Heat shock protein; Stress protein; Mitochondria; シグナルペプチド; ミトコンドリア移行

Prokaryotic chaperonin has been well characterized, and the crystal structure of the asymmetric GroEL-GroES-(ADP)_7 chaperonin complex has been shown by Xu et al. On the contrary, biochemical properties of mammalian chaperonin homolog (HSP60/10) have not yet fully understood.We have analyzed binding protein to the signal sequence of HSP60 using signal sequence affinity column chromatography and identified only one protein with molecular mass of 70kDa. The protein was identified as HSP70 on immunoblotting using an antibody against HSP70.We have also investigated import of HSP60 into mitochondria in vivo. The mRNA of HSP60 was.increased in the papilla of water- restricted rat kidneys for 3 or 5 days. On immunoblotting analysis, HSP60 was detected both in cytoplasm and mitochondria of normal rat kidney papilla. Although the quantity of the protein was decreased in the cytoplasm of water-restricted rat kidney papilla, the protein was increased in the mitochondria of the papilla.These results suggested that mammalian molecular chaperon HSP60 is located both in the cytoplasm and mitochondria under normal conditions, and that cytoplasmic HSP60 is imported into mitochondria under severe conditions through cytoplasmic HSP70.

核核酸蛋白伴侣蛋白已经得到很好的特征，Xu等人已经显示了不对称的凹槽 - 胶质 - （ADP）_7伴侣蛋白复合物的晶体结构。相反，哺乳动物伴侣蛋白同源物（HSP60/10）的生化特性尚未完全理解。我们使用信号序列亲和力色谱柱色谱法分析了与HSP60的信号序列的结合，并仅鉴定出一种用一种70KDA的分子质量鉴定出一种蛋白质。使用针对HSP70的抗体，我们还研究了HSP60进口体内的线粒体，将蛋白鉴定为HSP70。 Hsp60的mRNA在水受限的大鼠肾脏的乳头状含量为3或5天。在免疫印迹分析中，在正常大鼠肾乳头的细胞质和线粒体中都检测到HSP60。尽管在限制水的大鼠肾乳头的细胞质中蛋白质的量减少，但蛋白质在乳头的线粒体中增加了蛋白质。这些结果表明，这些结果表明，在正常情况下，在细胞质中，哺乳动物分子伴侣HSP60均位于细胞质和线粒体中，并将​​其导入了hsp60 ss60，细胞质HSP70。

项目成果

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Kawamura, K.: "Chaperonin 10 in the rat oocyttes and erly embryos : its expression and activity for early pregnancy factor"Am. J. Reprod. Immunol. 44. 242-248 (2000)

Kawamura, K.：“大鼠卵母细胞和早期胚胎中的伴侣蛋白 10：其早孕因子的表达和活性”Am。

Iwabuchi A.: "Specific preinduction of 60-kDa heat shcok protein (chaperonin homolog) by TRH does not protect clonic mucosa against acetic acid-induced lesion in rat"Digest. Dis. Sci. 45. 1480-1489 (2000)

Iwabuchi A.：“TRH 对 60-kDa 热休克蛋白（伴侣蛋白同系物）的特异性预诱导不会保护大鼠中阵挛粘膜免受乙酸诱导的病变”文摘。

Motoyama, S: "Hydrdrogen peroxide induces midzonal heat shock protein 72 and apoptosis in sinusoidal endothelial cells of hypoxic rat liver"Crit. Care Med.. 28. 1509-1514 (2000)

Motoyama, S：“过氧化氢诱导缺氧大鼠肝脏中区热休克蛋白 72 和肝窦内皮细胞凋亡”Crit。

Iwabuchi, A.: "Specific preinduction of 60-kDa heat shock protein (chaperonin homolog) by TRH does not protect clonic mucosa against acetic acid-induced lesion in rat"Digest. Dis. Sci.. 45. 1480-1489 (2000)

Iwabuchi, A.：“TRH 对 60-kDa 热休克蛋白（伴侣蛋白同源物）的特异性预诱导不会保护克隆粘膜免受乙酸诱导的大鼠病变”文摘。