Molecular Biologic Study on Etiological Mechanisms and Therapy of Glaucoma

1)免疫组化检测MYOC/TIGR蛋白在青光眼和正常小梁网中的定位。光镜免疫组化，所有标本的小梁网抗myoc /TIGR多克隆抗体染色阳性。细胞外基质染色。电镜免疫组化染色不仅可见于小梁细胞的细胞质，而且可见于细胞外基质。在细胞外基质中，染色与长间距胶原和细颗粒物质有关。结果表明，MYOC/TIGR蛋白不仅分布在小梁细胞中，还分布在与长间距胶原和细颗粒物质相关的细胞外基质中。2)对去核脱落综合征人眼小梁组织进行组织学和免疫组织化学检查。脱落纤维被发现含有蛋白聚糖以及一些其他大分子。该综合征患者的虹膜血管也出现了一些异常变化。3)对家兔遗传性青光眼的眼角区进行形态学观察。青光眼的角状区位于神经丛下方，细胞外基质内嵌有一厚的异常组织，呈圆形。厚组织中可见大量基底层样细胞外基质。在正常眼睛中，角状区域由发育良好的小梁片组成。这些发现支持了一种假设，即虹膜角膜角发育不良导致的小管下厚组织的残留是这种类型青光眼的主要原因之一。4)我们对新生血管性青光眼人和兔眼前段新生血管的小梁网进行了组织学和免疫组织化学的观察。结果提示生长因子（VEGF）在眼前段发育中起重要作用，新生小梁间隙的侵袭与青光眼的表现密切相关。5)用免疫组化方法研究了正常细胞外基质的定位。结果表明，层粘连蛋白和VI型胶原弥漫性分布于小梁网。6)我们检查了左眼虹膜痣综合征患者小梁切除术后获得的小梁组织，表明虹膜痣综合征可能发生血水屏障的破坏。7)采用激光多普勒血流仪检测富马酸溴长春胺和马来酸噻洛尔对脉络膜血流的影响。结果表明，静脉给药可能不会增加脉络膜血流量。少

1) We immunohistochemically examined the localization of MYOC/TIGR protein in the glaucomatous and normal trabecular meshworks. In light microscopic immunohistochemistry, the trabecular meshworks from all the specimens stained positively with anti-MYOC/TIGR polyclonal antibody. Staining was observed in the extracellular matrix. In electron microscopic immunohistochemistry, staining was seen not only in the cytoplasm of the trabecular cells but also in the extracellular matrix. In the extracellular matrix, staining was associated with the long-spacing collagens and fine granular materials. It is concluded that MYOC/TIGR protein is distributed not only in the trabecular cells but also in the extracellular matrix associating with the long-spacing collagens and fine granular materials.2) The trabecular tissues from enucleated human eyes with exfoliation syndrome were examined histologically and immunohistochemically. Exfoliation fibers were revealed to contain proteoglycans as well as some … More other macromolecules. It was also detected that the iris vessels had some abnormal changes in the syndrome.3) We examined morphologically the angular region of eyes with inherited glaucoma in rabbits. In the angular region of glaucomatous eyes, a thick abnormal tissue with round-formed cells embedded in the extracellular matrix was located just beneath the plexus. A large amount of extracellular matrix of basal lamina-like material was observed in the thick tissue. In normal eyes, the angular region consisted of well-developed trabecular sheets. These findings support the hypothesis that remaining of a thick subcanalicular tissue because of maldevelopment of the iridocorneal angle is one of the main causes of this type of glaucoma.4) We examined the trabecular meshwork from human eyes with neovascular glaucoma, as well as from rabbit eyes with experimental neovascularization in the anterior segment of the eye histologically and immunohistochemically. The results suggested growth factor (VEGF) plays an important role in development of anterior segment of the eye, and that invasion of the newly intertrabecular spaces has close relation to glaucoma manifestation.5) We studied the localization of extracellular matrix in the normal immunohistochemically. The results indicate that laminin and type VI collagen are located diffusely in the trabecular meshwork.6) We examined the trabecular tissue obtained by trabeculectomy from the patient's with iris nevus syndrome in the left eye to show that disruption of the blood-aqueous barrier may occur in iris-nevus syndrome.7) We examined the effects of brovincamine fumarate and timolol maleate on choroidal blood flow using laser Doppler flowmetry. The results indicated that intravenous administration of the drugs might not increase the choroidal blood flow. Less