Activation of T and B lymphocytes by oral mycoplasmas

口腔支原体对 T 和 B 淋巴细胞的激活

• 批准号: 07672035
• 负责人: SHIBATA Kenichiro
• 金额: $ 1.47万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672035/
• 关键词: mycoplasma; phospholipase C; T cells; apoptosis; TNF-alpha; fibroblast; IL-6; IL-8; TNFα; 口腔マイコプラズマ; コンカナバリンA

1. AIDS-associated mycoplasmas possessed phosphatidylcholine- and phosphatidylinositol (PI) -specific phospholipases C (PLC) in the membrane. PLC are shown to be a virulence determinant of bacteria. Especially, PI-PLC are known to release GPI-anchored proteins such as CD14, CD55 and Fc receptor which play important roles in the defense system of the host.2. Oral and AIDS-associated mycoplasmas possessed the activity to enhance concanavalin A-induced apoptosis of mouse splenic T cells. TNF-alpha was found to play an key role in the expression of the activity.3. Oral mycoplasmas induces interleukin-6 and interleukin-8 in human gingival fibroblasts by a mechanism different from that of lipopolysaccharides of gram-negative bacteria.

1.艾滋病相关的支原体具有磷脂酰胆碱和磷脂酰肌醇（PI）特异性磷脂酶C（PLC）的膜。PLC被证明是细菌的毒力决定因子。尤其是PI-PLC可以释放GPI锚定的蛋白，如CD 14、CD 55和Fc受体，这些蛋白在宿主的防御系统中起重要作用.口服和艾滋病相关支原体具有增强刀豆球蛋白A诱导的小鼠脾T细胞凋亡的活性。发现TNF-α在该活性的表达中起关键作用.口腔支原体通过与革兰氏阴性菌脂多糖不同的机制诱导人牙龈成纤维细胞产生白细胞介素-6和白细胞介素-8。

项目成果

柴田健一郎,渡邊継男: "Mycoplasma fermentans enhances concanavalin A-induced apoptosis of mouse splenic T cells" FEMS Immunology and Medical Microbiology. 17. 103-109 (1997)

Kenichiro Shibata、Ttsuguo Watanabe：“发酵支原体增强伴刀豆球蛋白 A 诱导的小鼠脾 T 细胞凋亡”FEMS 免疫学和医学微生物学 17. 103-109 (1997)。

柴田健一郎,佐々木次雄,渡邊継男: "AIDS-associated mycoplasmas possess phospholipases C in the membrane" Infection and Immunity. 63. 4174-4177 (1995)

Kenichiro Shibata、Ttsuguo Sasaki、Tsuguo Watanabe：“艾滋病相关支原体在膜中具有磷脂酶 C”《感染与免疫》63. 4174-4177 (1995)。

柴田健一郎、渡邊継男: "Mycoplasma fermentans enhances concavalin A-induced apoptosis of mouse splenic T cells" FEMS Immunology and Medical Microbiology. 17・2. 103-109 (1997)

Kenichiro Shibata、Tsuguo Watanabe：“发酵支原体增强伴刀豆球蛋白 A 诱导的小鼠脾 T 细胞凋亡”FEMS 免疫学和医学微生物学 17・2（1997）。

Ken-ichiro Shibata, Tsuguo Sasaki, Tsuguo Watanabe: "AIDS-associated mycoplasmas possess phospholipases C in the membrane" Infection and Immunity. 63. 4174-4177 (1995)

Ken-ichiro Shibata、Ttsuguo Sasaki、Ttsuguo Watanabe：“艾滋病相关支原体在膜中拥有磷脂酶 C”感染和免疫。

Ken-ichiro Shibata, Tsuguo Watanabe: "Mycoplasma fermentans enhances concanavalin A-induced apoptosis of mouse splenic T cells" FEMS Immunology and Medical Microbiology. 17. 103-109 (1997)

Ken-ichiro Shibata、Ttsuguo Watanabe：“发酵支原体增强刀豆球蛋白 A 诱导的小鼠脾 T 细胞凋亡”FEMS 免疫学和医学微生物学。