Functions and Analytical applications of Carriers Modified with Metalloporphyrins as Artificial Solid Mimesis of Enzymes.

(1)通过酰胺键将金属卟啉固定在硅胶和玻璃微珠上。改性载体（MPs和MPg）在2年的储存和水分方面都是稳定的。(2)利用过氧化物酶催化的显色反应研究了MPs和MPg对过氧化物的类过氧化物酶催化活性，结果表明，部分MPs和MPg既具有过氧化物酶样活性，又具有过氧化物酶样活性。该活性适用于过氧化氢的测定。我们试图开发一种新的方法来分析过氧化氢的流动。它是基于被锰卟啉修饰的载体的过氧化物酶样活性。此外，我们开发了一种有机过氧化物的流动分析方法，其中用钴卟啉修饰的载体作为催化剂。(4)用钴卟啉固定硅胶可加速抗坏血酸氧化酶催化的氧化还原反应。该载体经Co^<3+>-卟啉修饰后可作为固体催化剂用于测定抗坏血酸。(5)经金属卟啉修饰的离子交换树脂具有类似谷胱甘肽过氧化物酶的活性。该树脂有望作为固定化谷胱甘肽过氧化物酶的良好模拟物在实际应用中发挥作用。此外，经Co^<3+>-卟啉修饰的载体可加速谷胱甘肽氧化酶催化的氧化还原反应。(6)八溴锰卟啉的共振拉曼光谱表明，修饰载体的过氧化物酶样活性是由于Mn^<3+> * Mn^<4+>的氧化还原循环。(7) a)基于mn -卟啉类过氧化物酶的活性，研制了一种新的三碘离子离子选择电极。b)以金属卟啉修饰的硅胶填充的色谱柱可能有利于作为基于pi-pi电子相互作用的固定相。

(1) Metallo-porphyrins were immobilized to silica gels and glass beads through amide-bonds. The modified carriers (MPs and MPg) were revealed to be stable in terms of storage and moisture for 2 years.(2) Peroxidase-like catalytic activities of MPs and MPg for peroxides were investigated by using a color reaction catalyzed by peroxidase : Consequently, some of MPs and MPg was revealed to exhibit the peroxidase-like activity as well as peroxidase. This activity is applicable to determination of hydrogen peroxide.(3) We have attempted to develop a new method for a flow analysis of hydrogen peroxide. which is based on peroxidase-like activity of a carrier modified with Mn-porhyrin. Furthermore, we developed a flow analysis method for organic peroxide, where a carrier modified with cobalt-porphyrin acts as a catalysis.(4) Silica gels immobilized with cobalt-porphyrin proved to accelerate the redox reaction catalyzed by ascorbate-oxidase. The carries modified with Co^<3+>-porphine could be expected to be useful practically as a solid catalyst for the determination of ascorbate.(5) Some of the ion-exchange resins modified with metalloporphyrins were found to exhibit glutathione-peroxidase-like activities. The resins were expected to be useful practically as a good mimesis in place of immobilized glutathione-peroxidase. In addition, the carriers modified with Co^<3+>-porphyrin proved to accelerate the redox reaction catalyzed by glutathione-oxidase.(6) The resonance Raman spectra of octabromo-Mn-porphyrin indicated that an peroxidase-like activity of the modified carrier is due to the redox cycle, Mn^<3+> * Mn^<4+>.(7) a) A new ion selective electrode for triiodide ion has been developed, and is based on the peroxidase-like activity of Mn-porphyrins. b) A column packed with silica gels modified with metalloporphyrins may be advantageous as a stationary phase based on pi-pi electron interaction.