Elucidation of the mechanism of functional expression of metal-porphyrin immobilized carrier as an artificial solid catalyst.

阐明金属卟啉固定载体作为人工固体催化剂的功能表达机制。

• 批准号: 18590033
• 负责人: SAITO Yutaka
• 金额: $ 2.14万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590033/
• 关键词: Metal-porphyrin; Immobilized carrier; Artificial solid catalyst; Adrenaline; Peroxidase; Vibrational spectrum; Resonance Raman spectrum; Mechanism; 固体化担体; 人工個体触媒; ヘテロサイクリックアミン

The aim of this study is to teach measurement skill to young researchers through development of artificial catalyst and elucidation of the mechanism of catalytic activity. Although applicant has already developed several artificial catalysts, the mechanism of the catalysts has not been completely elucidated. Then, development of new artificial catalysts and elucidation of the mechanism were attempted. The results obtained in this research are listed below.1. Carriers modified with Mn-porphyrin as an artificial catalyst for oxidation of catecholamines such as adrenaline were developed. The carriers can oxidize adrenaline to adrenochrome and adrenolutin. It was suggested that this oxidative catalytic function is attributable to the redox cycle of Mn^<3+> 〓 Mn^<4+>2. To develop a new application of metal-porphyrin immobilized carrier, its applicability to the stationary phase for HPLC was investigated.3. The peroxidase-like activity of metal-octabromo-porphyrin such as Fe^<3+>-OBTM, Co^<3+>-OBTM and Mn^<3+>-OBTM on organic peroxide was examined. It was revealed that Fe^<3+>-OBTM has an activity equivalent to conventional enzyme. To elucidate the mechanism of peroxidase-like activity of Fe^<3+>-OBTM by resonance Raman spectra, the flow cell system was developed. The results obtained in this study revealed that said activity is attributable to the repeated redox cycle of the central metal of Fe^<3+>-OBTM, i.e., Fe^<3+>〓Fe^<4+>.The measurement skill for the vibrational spectra such as the resonance Raman specrreum could be imparted to young researchers through the research from 2006 to 2007.

本研究的目的是通过人工催化剂的开发和催化活性机理的阐明，向年轻的研究人员传授测量技能。虽然申请人已经开发了几种人工催化剂，但催化剂的机理尚未完全阐明。在此基础上，对新型人工催化剂的开发和机理的阐明进行了尝试。本研究取得的成果如下.开发了一种锰卟啉修饰的人工催化剂载体，用于肾上腺素等儿茶酚胺的氧化。载体可将肾上腺素氧化为肾上腺素红和肾上腺素。这种氧化催化作用可能是由于Mn^<3+> → Mn^<4+>2的氧化还原循环所致.为开发金属卟啉固定化载体的新用途，考察了其作为高效液相色谱固定相的适用性.研究了Fe^<3+>-OBTM、Co^<3+>-OBTM和Mn^<3+>-OBTM等金属八溴卟啉对有机过氧化物的类过氧化物酶活性。结果表明，Fe^<3+>-OBTM具有与常规酶相当的活性。为了通过共振拉曼光谱研究Fe^<3+>-OBTM的类过氧化物酶活性机制，我们建立了流动池系统。本研究中获得的结果表明，所述活性归因于Fe^<3+>-OBTM的中心金属的重复氧化还原循环，即，Fe^<3+> → Fe^<4+>，通过2006 - 2007年的研究，可以将共振拉曼光谱等振动光谱的测量技巧传授给年轻的研究人员。

项目成果

Catalytic Activity of Silica Gels Bound Manganese (III)-Porphyrin on Oxidative Reaction of Adrenaline

硅胶结合锰(III)-卟啉对肾上腺素氧化反应的催化活性

• 发表时间: 2007
• 期刊: Chem. Pharm. Bull. 55(3)
• 作者: Kitamura Y.;Takatsuki T.;Kawamoto M.;Saito M.;Iwado A.;Tsukamoto I.;Mifune M.;Saito Y.
• 通讯作者: Saito Y.

Evaluation of the State of Active Ingredients in Pharmaceutical Preparations Using Fourier Transform-Raman Difference Spectra

利用傅里叶变换-拉曼差分光谱评价药物制剂中活性成分的状态

• 发表时间: 2006
• 期刊: YAKUGAKU ZASSHI 126(12)
• 作者: Mifune;M.;Iwasaki;T.;Kozaki;Y.;Tsukamoto;I.;Saito;M.;Kitamura;Y.;Yamaguchi;T.;Saito;Y
• 通讯作者: Y

Standard infrared absorption spectrum of betaine and optimal conditions for its measurement

• DOI: 10.3358/shokueishi.47.232
• 发表时间: 2006-10-01
• 期刊: JOURNAL OF THE FOOD HYGIENIC SOCIETY OF JAPAN
• 作者: Kitamura, Youji;Iwasaki, Toshinobu;Tanamoto, Kenichi
• 通讯作者: Tanamoto, Kenichi

Peroxidase-like catalytic activity of Mn- and Fe-tetrakis-(4-carboxyphenyl)porphines bound to aminopropyl-glass bead in oxidative reaction of heterocyclic amines

氨丙基玻璃珠上Mn-和Fe-四-(4-羧基苯基)卟啉在杂环胺氧化反应中的类过氧化物酶催化活性

• 发表时间: 2006
• 期刊: Talanta 69(1)
• 作者: Kitamura;Y.;Mifune;M.;Hino;D.;Yokotani;S.;Saito;M.;Tsukamoto;I.;Iwado;A.;Saito;Y
• 通讯作者: Y

Effect of octabromination of a tetrakis(4-crboxyphenyl)porphine derivative bound to silica gels on HPLC retention behaviors of polyaromatic hydrocarbons

硅胶上四(4-羧基苯基)卟啉衍生物八溴化对多环芳烃 HPLC 保留行为的影响

• 发表时间: 2006
• 期刊: Talanta. 69(5)
• 作者: Kitamura Y.;Kawata K.;Tanaka K.;Furuyashiki Y.;Mifune M.;Tsukamoto I.;Saito M.;Haginaka J.;Saito Y.
• 通讯作者: Saito Y.