Antioxidative substance in sea weeds and the activity to inhibit oxidative damage

海藻中的抗氧化物质及其抑制氧化损伤的活性

• 批准号: 07680066
• 负责人: NISHIBORI Sukie
• 金额: $ 0.77万
• 依托单位: Tokaigakuen Women's College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680066/
• 关键词: antioxidative activity; lipid peroxidation; binding activity of active oxidant; TBA method; sea weed; xanthine; xanthine oxidase system; pheophytin a; derivative of chlorophyll a; キサンチン・キサンチンオキシダーゼ系; 鉄・過酸化水素系; HPLC; クロロフィル関連物質; 抗酸化性; 鉄過酸化水素系

We investigated the binding activity of seaweeds against active oxidant. Seaweeds were powdered by a blender, and extracted with chloroform and methanol. The extracts were classified into the polar or nonpolar compounds with the solvents by a open column. The activity of extracts inhibited active oxidant were measured by chemiluminesence method with xanthine/xanthine oxidase system. The activity inhibited lipid peroxidation were by TBA method with the erythrocyte membrane goust system.In the screening test, some compounds in the polar or nonpolar section showed the activity inhibited active oxidant. Further, the active compounds were fractionated by HPLC.Dark green fraction showed the strong antioxidative activity, and the fraction was separated and purified. The compound was isolated as pheophytin a. Pheophytin a was compared with beta-carotene about the activity inhibited active oxidant in the point of the contribution to the human health and body.

我们研究了海藻对活性氧化剂的结合活性。将海藻用搅拌机打成粉末，并用氯仿和甲醇提取。通过开柱将提取物与溶剂分类为极性或非极性化合物。采用黄嘌呤/黄嘌呤氧化酶系统化学发光法测定提取物抑制活性氧化剂的活性。抑制脂质过氧化的活性是用红细胞膜古斯特系统进行TBA法。在筛选试验中，一些化合物在极性或非极性部分表现出抑制活性氧化剂的活性。进一步，通过HPLC对活性化合物进行分级分离，深绿色组分表现出较强的抗氧化活性，对该组分进行分离纯化。该化合物被分离为脱镁叶绿素a。从对人类健康和身体的贡献角度，将脱镁叶绿素a与β-胡萝卜素的活性抑制活性氧化剂进行了比较。