Antioxidative substance in sea weeds and the activity to inhibit oxidative damage

研究了海藻对活性氧化剂的结合活性。海藻用搅拌器磨成粉末，用氯仿和甲醇提取。用开孔柱将萃取物分为极性化合物和非极性化合物。采用黄嘌呤/黄嘌呤氧化酶体系化学发光法测定抗活性氧化剂提取物的活性。用TBA法测定其对脂质过氧化的抑制作用。在筛选试验中，极性和非极性部分化合物表现出抑制活性氧化剂的活性。采用高效液相色谱法对活性成分进行分离。深绿色部分显示出较强的抗氧化活性，并对其进行了分离纯化。分离得到该化合物为叶绿素a，比较了叶绿素a与β -胡萝卜素在抑制活性氧化剂方面的活性，以及对人体健康和机体的贡献。

We investigated the binding activity of seaweeds against active oxidant. Seaweeds were powdered by a blender, and extracted with chloroform and methanol. The extracts were classified into the polar or nonpolar compounds with the solvents by a open column. The activity of extracts inhibited active oxidant were measured by chemiluminesence method with xanthine/xanthine oxidase system. The activity inhibited lipid peroxidation were by TBA method with the erythrocyte membrane goust system.In the screening test, some compounds in the polar or nonpolar section showed the activity inhibited active oxidant. Further, the active compounds were fractionated by HPLC.Dark green fraction showed the strong antioxidative activity, and the fraction was separated and purified. The compound was isolated as pheophytin a. Pheophytin a was compared with beta-carotene about the activity inhibited active oxidant in the point of the contribution to the human health and body.