Confocal/Multiphoton Microscope Upgrade

共焦/多光子显微镜升级

• 批准号: 8826419
• 负责人: John J Lemasters
• 金额: $ 57.05万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-02-15 至 2016-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8826419
• 关键词: 8 year old; Cancer Center; Cell Death; Computer software; Cost Savings; Detection; Environment; Equipment; Goals; Image; Imaging Techniques; Immersion Investigative Technique; Individual; Injury; Lasers; Medical; Microscope; Microscopy; Natural regeneration; Optics; Performance; Research Personnel; Scanning; South Carolina; System; Training; Universities; Water; Work; base; cellular imaging; detector; in vivo imaging; molecular imaging; optical imaging; vibration

 DESCRIPTION (provided by applicant): The Cell and Molecular Imaging (CMI) Facility is a shared component of the Center for Cell Death, Injury and Regeneration (CCDIR) and the NCI-designated Hollings Cancer Center (HCC) whose purpose is to provide CCDIR/HCC investigators and other researchers at the Medical University of South Carolina (MUSC) with equipment and expertise for laser scanning confocal/multiphoton microscopy and related high-end optical imaging techniques. The only access of our investigators to combined confocal/mulitphoton microscopy is an 8+ year old Zeiss 510 NLO META microscope that is deficient in important functionalities that are essential for high performance confocal/multiphoton and intravital (in vivo) imaging, including spectral detection, long working distance water immersion optics and maximally sensitive descanned and nondescanned detection. Accordingly, the overall goal of this application is to upgrade the Zeiss 510 NLO microscope to an optimally configured and fully functional confocal/multiphoton microscope system to be made available to CCDIR, HCC and MUSC investigators. We propose to upgrade this system to a Zeiss 780 confocal/multiphoton inverted laser scanning microscope with maximally sensitive 3-channel non-descanned GaAsP detectors, a GaAsP-based 34-channel spectral array detector, 6 visible laser lines for confocal imaging, high performance long working distance intravital water-immersion optics and Zen software. Cost savings will be achieved by using microscope objectives, multiphoton laser, vibration isolation table and environmental chamber from the old microscope, whose remaining components will be traded in. The upgraded microscope system will be placed in a multiuser environment that provides technical support, user training and applications expertise in support of individual user projects.