MRI: Acquisition of A Multiphoton Confocal Laser Scanning Microscope for Life Science and Biomedical Research and Training at SUNY Binghamton

该计划旨在购买一台蔡司LSM 880 NOL三通道多光子共聚焦激光扫描显微镜，以升级纽约州立大学宾厄姆顿大学(BU)已有八年历史的徕卡SP 5单光子共聚焦显微镜，该显微镜帮助宾夕法尼亚大学的教职员工成功开发了一流的研究项目。现有的显微镜最初是为薄组织样本和微生物成像而配置的。随着波士顿大学神经科学、干细胞和再生医学、3D组织生物打印、纳米医学等研究项目的扩大，该大学迫切需要一种更先进的多光子共聚焦显微镜来进行活细胞成像。LSM880NOL是一种能够进行亚细胞成像的双光子共聚焦显微镜。这一最先进的显微镜的使用将推动波士顿大学最近的健康科学倡议，提高教师在生物医学工程、生物科学、化学和心理学系以及新成立的药学院的研究能力。它还将帮助校园里更多的人获得初步结果，以寻求NSF和NIH的资金。该项目的结果将对我们对细胞和蛋白质在体内的动态行为的基本理解产生更广泛的影响。该项目解决了在单细胞和单分子水平上研究活细胞、组织和小动物的细胞生物学、基因调控、神经元激活和组织再生/重塑的未得到满足的需求。此外，它的收购将促进BU与行业的合作，以加快将生命科学和生物医学工程发现转化为生物产品。一个全面的管理计划将确保仪器始终在高峰和接近高峰时运行。来自波士顿大学和参与该项目的研究人员的强大财务和管理承诺将在项目结束后维持该设施。该提案寻求购买蔡司LSM 880 NOL三通道多光子共聚焦激光扫描显微镜，以升级纽约州立大学宾厄姆顿大学(BU)已有8年历史的徕卡SP 5单光子共聚焦显微镜，该显微镜帮助波士顿大学的教职员工成功开发了一流的研究项目。LSM880NOL是一台双光子共焦显微镜。它的脉冲超快红外激光只在焦点激发荧光染料或蛋白质，那里的光子密度足够高，足以产生荧光。红色激发光可以更深地穿透到接近1毫米的组织中。它可以横向分辨率140 nm，轴向分辨率400 nm，分辨率为488 nm，允许亚细胞成像。广泛的研究兴趣，加上目前参与该项目的研究人员的资金，迫切需要在波士顿大学购买显微镜。这一先进的多光子显微镜的获得将立即影响到三所学校六个系的17个研究实验室。一旦该系统在校园内建立起来，更多的实验室将受到影响。参与这一项目的研究人员得到了美国国家科学基金会、美国国立卫生研究院等机构的大力资助，他们的研究项目都与显微镜直接相关。收购该仪器不仅将使他们能够与校园、州和全国的研究人员开发更多的合作项目，而且还将使教师能够追求新的研究方向，并为波士顿大学的研究和学术项目的增长做出巨大贡献。它将使研究人员能够研究细胞-细胞和细胞-细胞外基质之间的相互作用；跟踪细胞的迁移、分化和增殖；研究伤口愈合、器官移植和组织再生/重建过程中的细胞招募；以及测量活细胞、组织、3D打印有机物或小动物内的扩散、受体/配体相互作用等生化反应。从这些研究中获得的知识将大大促进生物科学和生物工程的发展。显微镜的获得将推动和加速这些进步。它将通过提供一个对校园、州和全国的所有研究人员和学生开放的多用户成像设施，显著加强波士顿大学在生命科学和生物医学工程方面的研究基础设施。此外，它还将为现代生物学和生物工程的教学提供强大的资源。将开发一门新的实验课程，重点放在活细胞和组织多光子显微镜上，并向本科生和研究生提供。将举办讲习班和开放日，以培训和吸引新的用户接触显微镜，并接触到K-12和当地社区，特别是来自代表性不足群体的社区。它将为夏季REU学生提供额外的研究培训机会。最后，它将成为吸引顶尖教师到该大学的绝佳招聘工具。

This proposal seeks to acquire a Zeiss LSM 880 NOL 3-channel multiphoton confocal laser scanning microscope to upgrade SUNY Binghamton University's (BU) eight-year-old Leica SP 5 single photon confocal microscope that has helped BU faculty to successfully develop top-ranked research programs. The existing microscope was originally configured for thin tissue sample and microorganism imaging. With the expansion of BU research programs in neuroscience, stem cell and regenerative medicine, 3D tissue bioprinting, nanomedicine, etc., the University is in urgent need of a more advanced multiphoton confocal microscope for live cell imaging. LSM880 NOL is a two photon confocal microscope capable of subcellular imaging. Access to this state-of-the-art microscope will propel BU's recent Health Science Initiative, boost faculty research capabilities in Biomedical Engineering, Biological Science, Chemistry, and Psychology Departments and the newly-formed Pharmacy School. It will also help more individuals on campus obtain preliminary results to seek for NSF and NIH funding. The outcome of this project would have a broader impact on our fundamental understanding of how cell and protein behave dynamically in vivo. This project address unmet needs to study cell biology, gene regulation, neuron firing, and tissue regeneration/remodeling in live cells, tissues, and small animals at single cell and single molecule level. Furthermore, its acquisition will foster BU's collaboration with industries to accelerate the translation of life science and biomedical engineering discoveries into bioproducts. A comprehensive management plan will ensure that the instrument always runs at peak and near peak capacity. The strong financial and management commitment from BU and the researchers involved in this project will sustain the facility after the project period.This proposal seeks to acquire a Zeiss LSM 880 NOL 3-channel multiphoton confocal laser scanning microscope to upgrade SUNY Binghamton University's (BU) eight-year-old Leica SP 5 single photon confocal microscope that has helped BU faculty to successfully develop top-ranked research programs. LSM880 NOL is a two photon confocal microscope. Its pulsed, ultrafast infrared laser excites fluorescent dyes or proteins only at the focal point, where photon density is high enough to produce fluorescence. The red excitation light penetrates much deeper into tissue close to 1 mm. It can resolve 140 nm laterally and 400 nm axially at 488 nm, allowing for subcellular imaging. The breadth of research interests, coupled with the current funding of investigators involved in this project poses urgent need for the acquisition of the microscope at BU. The acquisition of this advanced multiphoton microscope will immediately affect more than 17 research labs across six Departments in three schools. More labs will be impacted once the system is set up on campus. The investigators involved in this project are well funded by NSF, NIH, etc. and have research projects that are directly related to the microscope. The acquisition of the instrument will not only permit them to develop more collaborative projects with researchers across the campus, state and nation, but it will also allow faculty to pursue new directions of research and contribute greatly to the growth of BU's research and academic programs. It will enable investigators to study interaction between cell-cell and cell-extracellular matrix; to track cell migration, differentiation, and proliferation; to investigate cell recruitment during wound healing, organ transplantation and tissue regeneration/remodeling; and to measure biochemical reactions such as diffusion, receptors/ligand interaction within live cells, tissues, 3D printed organoids or small animals. Knowledge gained from these studies will significantly advance both bioscience and bioengineering. The acquisition of the microscope will propel and accelerate these advances. It will remarkably enhance BU's research infrastructure in life science and biomedical engineering by providing a multiuser imaging facility that is open to all investigators and students across the campus, state, and nation. Furthermore, it will provide a powerful resource for teaching modern biology and bioengineering. A new lab course focusing on live cell and tissue multiphoton microscopy will be developed and offer to both undergraduate and graduate students. Workshops and open house will be held to train and attract new users to access to the microscope and to reach out to K-12 and local communities, especially those from underrepresented groups. It will offer additional research training opportunities for summer REU students. Finally, it will serve as an excellent recruiting tool for attracting top-notch faculty to the university.