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Mating type and variation of Phytophthora infestans in Asia

亚洲致病疫霉的交配类型和变异

基本信息

批准号：
08041129
负责人：
OGOSHI Akira
金额：
$ 5.25万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

Phytophthora infestans isolates collected from potato or tomato fields in seven Asian countries were analyzed for mating type, metalaxyl sensitivity, isozyme genotypes and mtDNA types. All of the isolates collected in India, Taiwan and the northern China were A1 mating type, and all of the isolates collected in Korea and Indonesia were A2 mating type, while isolates of both mating types were found in samples from Thailand, Nepal, the central inland China. All isolates from India, Thailand, Nepal and the southern China, and most of isolates collected in Taiwan and the central China were sensitive to metalaxyl, while all except one isolate collected in Korea, all of the isolates collected in Indonesia and 11 of 41 isolates collected in the northern China were resistant. Isolates were divided into 6 isozyme genotypes : Types A (42 isolates from Taiwan, 23 from Thailand, 46 from Nepal, 1 from the northern China and 2 from the southern China), B (12 from India and 18 from Nepal), C (32 from Thailand and 34 from Nepal), D (16 from Korea and 4 from Indonesia), E (43 from the central China and 14 from the southern China), and F (40 from the northern China and 9 from the central China). A and B type isolates were of the old population that was predominant worldwide until recently. C type isolates belonged to the new population, which has been detected in the world since the appearance of A2 isolates outside central Mexico. D type isolates has not been detected elsewhere except Poland and Japan. E and F type isolates were also specific to Asia. Isolates were divided into mtDNA types Ia, Ib and IIa by RFLPs of mtDNA. There were relationships between mtDNA types and isozyme genotypes except for a few isolates. The isolates of type IIa were from isozyme genotype B, C or E type, the isolates of type Ib were of A type, and the isolates of type IIa belonged to D or F type.

对亚洲7个国家马铃薯或番茄田分离的疫霉菌进行了配偶型、甲轴酯敏感性、同工酶基因型和mtDNA型分析。分离株分为6种同工酶基因型：A型（台湾42株，泰国23株，尼泊尔46株，中国北方1株，中国南方2株），B型（印度12株，尼泊尔18株），C型（泰国32株，尼泊尔34株），D型（韩国16株，印度尼西亚4株），E型（华中43株，华南14株），F型（华北40株，华中9株）。A型和B型分离株是直到最近才在世界范围内占主导地位的老种群。C型分离株属于新种群，自墨西哥中部以外出现A2型分离株以来，已在世界范围内发现。除波兰和日本外，其他地方未发现D型分离株。E型和F型分离株也是亚洲特有的。根据mtDNA的RFLPs将分离株分为Ia型、Ib型和IIa型。除少数分离株外，mtDNA类型与同工酶基因型之间存在相关性。IIa型分离株为同工酶基因型B、C或E型，Ib型分离株为A型，IIa型分离株为D或F型。