Studies on A1/A2 mating types in Phytophthora infestans

马铃薯晚疫病菌A1/A2交配型的研究

• 批准号: 08406003
• 负责人: OGOSHI Akira
• 金额: $ 23.17万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08406003/
• 关键词: Phytophthora infestans; pectate lyase; isoenzyme; new A1; ジャガイモ; 疫病; メタラキシル; メラタキシル; phytophthora; 交配型

Oosporogenesis of Phytophthora infestans occur in mating condition of A1 and A2 mating type strains. The differences of gene expression between mating and single cultures of A1 and A2 strains were displayed by cDNA representational difference analysis. Subtraction between mating and single cultures were done for three times by RDA.One hundred and twenty-eight clones were picked at random and probes of them were prepared for Northern analysis. It was shown that one of RDA clones (cET58) was up-regulated in mating condition. cDNA clone which isolated from cDNA library of mating cultured cells have 786 bp of open reading frame, and it is most similar to pectate lyase of Fusarium solani by database search of nucleotide and amino acid sequences. We designated this gene as mpl 1. Transcription of mpl 1 reaches its peak during five and six days after mating. Southern analysis on some isolates showed that mpl 1 is a multicopy gene, and the pattern of signal were typical in the groups of isolat … More es.The distribution of each mating type of Phytophthora infestans in Japan was as follows ; In 1999, all of Kyushu isolates were A2 mating type. In 2000, all of Hokkaido and Gunma isolates were A1 mating type. All of Hokkaido and Gunma isolates were metalaxyl- resistant, but there were resistant and susceptble isolates in Kyushu. In isoenzyme genotype, all of Kyushu isolates were D-type (Me=90/100, Gpi=100/100, Pep=96/96), and fifteen of Hokkaido isolates were F-type (Me=90/100, Gpi=98/98, Pep=100/100), and nine of Hokkaido isolates were G-type (Me=90/100, Gpi=100/100, Pep=98/98). All of Gunma isolates were F-type. As the result of DNA fingerprint was done using by RG52 probe, it was confirmed that Japanese old A1 isolates were same with US-1, and Japanese A2 isolates were consist of JP-1 and its mutants. Korea, India, Taiwan, Indonesia, ThaiLand, Nepal and China isolates were independently different. Both of mating types were exist in Northern China and Thailand. As the result of DNA fingerprint using by three kinds of probes, Japanese new A1 mating type isolates showed same difference with old A1 and A2 isolates. There is some possibility of being that new A1 isolates were the progenies of them. And it was showed that Japanese new A1 isolates were same as Northern Chinese isolates. Less

致病疫霉的卵孢子发生在A1和A2交配型菌株的交配条件下。cDNA代表性差异分析显示了A1和A2菌株交配与单培养间基因表达的差异。用RDA法对交配培养物和单培养物进行3次相减，随机挑取128个克隆，制备探针进行北方分析。结果表明，RDA克隆cET 58在交配条件下表达上调。从交配培养细胞的cDNA文库中分离得到的cDNA克隆具有786 bp的开放阅读框，通过数据库检索核苷酸和氨基酸序列，与茄病镰孢的果胶酸裂合酶最相似。我们将该基因命名为mpl 1。mpl 1的转录在交配后5 - 6天达到高峰。对部分菌株的Southern杂交分析表明，mpl 1基因是一个多拷贝基因，信号模式在菌株群中具有典型性 ...更多信息 致病疫霉各交配型在日本的分布如下：1999年九州分离物均为A2交配型。2000年北海道和群马分离物均为A1交配型。北海道和群马的菌株均对甲霜灵产生了抗性，但九州的菌株中既有抗性又有不敏感的菌株.在同功酶基因型上，九州分离株均为D型（Me=90/100，Gpi=100/100，Pep=96/96），北海道分离株15株为F型（Me=90/100，Gpi=98/98，Pep=100/100），北海道分离株9株为G型（Me=90/100，Gpi=100/100，Pep=98/98）。群马分离物均为F型。利用RG 52探针进行DNA指纹图谱分析，结果表明日本A1菌株与US-1菌株完全相同，日本A2菌株由JP-1菌株及其突变株组成。韩国、印度、台湾、印度尼西亚、泰国、尼泊尔和中国分离物是独立不同的。中国北方和泰国均存在这两种交配型。3种探针的DNA指纹图谱结果表明，日本新A1交配型菌株与老A1、A2菌株的DNA指纹图谱基本一致。A_1新菌株有可能是它们的后代。结果表明，日本新A1菌株与中国北方分离物的分离结果一致。少

项目成果

Fujii,N.,Akino,S.,Nishimura,R. and Ogoshi,A.: "Specifically isolated cDNA using cDNA-RDA from A2 mating type isolates of Phytophthora infestans"J.Fac.Agr.Hokkaido Univ. 69. 171-179 (1999)

藤井，N.，秋野，S.，西村，R.

Ryo Nishimura et al.: "Distribution of Phytophthora infestans populations in seven Asian countries" Ann.Phytopathol.Soc.Jpn.65. (1999)

Ryo Nishimura 等人：“七个亚洲国家致病疫霉种群的分布”Ann.Phytopathol.Soc.Jpn.65。

Naoya Fujii, et al.: "Specifically isolated cDNA using cDNA-RDA from A2 mating isolates of Phytophthora infestans"J. Fac. Agr. Hokkaido Univ.. 69. 171-179 (1999)

Naoya Fujii 等人：“使用 cDNA-RDA 从致病疫霉 A2 交配分离株中特异性分离 cDNA”J.

Fujii, N., Akino, S., Nishimura, R.and Ogoshi, A.: "Specifically isolated cDNA using cDNA-RDA from A2 mating type isolates of Phytophthora infestans"J.Fac.Agr.Hokkaido Univ. 69. 171-179 (1999)

Fujii, N.、Akino, S.、Nishimura, R. 和 Ogoshi, A.：“使用 cDNA-RDA 从致病疫霉 A2 交配型分离株中特异性分离 cDNA”J.Fac.Agr.北海道大学。

Ryo Nishimura, et al.: "Distribution of Phytophthora infestans populations in seven Asian countries"Ann. Phytopath. Soc. Jpn.. 65. 163-170 (1999)

Ryo Nishimura 等：“七个亚洲国家致病疫霉种群的分布”Ann。