Epidemiology and Control of Leishmaniasis in Cetral Eurasia ; A new epidemic in association with irrigation of deserts

欧亚大陆中部利什曼病的流行病学和控制；

• 批准号: 08041169
• 负责人: MATSUMOTO Yoshitsugu
• 金额: $ 8.38万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08041169/
• 关键词: Leishmaniasis; Central Eurasia; irrigation of deserts; Great gerbils; Zoonosis; Pathogenic protozoa; Molecular Epidemiology; Arthropod-borne Infection; 中華人民共和国; 中央アジア

An epidmiological research was undertaken in this year in Xinjinang Uyger Autonomous Region of the People's Republic of China. Twelve Great gerbils (Rhombomys opimus) were captured by traps, 101 female sandflies were collected from their burrows. Stamp smears were made from auricula, liver, and spleen of captured great gerbils, fixed with methanol, and stained with Giemsa. A part of the auricula was fixed with 10% buffered formalin and embeded in paraffin wax. Sections were made and stained with hematoxylin and eosin. Sandflies were dissected and their guts were examined microscopically for the presence of promastigotes. Sandflies guts in ehich promastigotes were obtained were transferred to tubes of NNN medium in order to isolate the parasites. As a result, six Leishmania isolates were obtained from three species of sandflies. Three isolates of Leishmania parasites were established from the auricula of the three gerbils. In histopathological examination of naturalli infected great ger … More bils,amastigotes were observed in the auricula but not in spleen and liver. No significant pathological changes were obtained. Eight inbred BALB/c mice and eight Mongolian gerbils were subcutaneously inoculated with eight isolates of Leishmania parasites, KMA-2, KMA-4 and KMA-7 (isolated from Great Gerbils) and KMP-1, KMP-2 and KMP-3, KMP-4 and KMP-6 (isolated from sandflies), pathological and histological examination were performed. Except for KMA-4, the BALB/c mice were shown to be resistant to every isolate. In contrast, high infectivity of Leishmania isolates except KMP-1 and KMP-6 was shown in the experiments on Mongolian gerbils. Promastigotes were successfully re-isolated from the noses of the Mongolian gerbils infected with each isolates. The mini-exon genes were analyzed for four isolates established. Based on the comparison of the sequences with those of reference strains of L. major, L. gerbilli, and L. turanica, Leishmania parasites isolated from the great gerbils were identified as L. gerbilli (KMA-4) and L. turanica (KMA-2), and parasites isolated from sandflies were identified as L. gerbilli (KMP-3) and L. turanica (KMP-2). Less

今年在中华人民共和国新疆南维吾尔自治区开展了一项流行病学研究。用陷阱捕捉大沙鼠（Rhombomys opimus）12只，从洞穴中收集雌性白蛉101只。用捕获的大沙鼠的耳廓、肝脏和脾脏制作邮票涂片，用甲醇固定，并用吉姆萨染色。将耳廓的一部分用10％缓冲福尔马林固定并包埋在石蜡中。制作切片并用苏木精和伊红染色。解剖白蛉并用显微镜检查其内脏是否存在前鞭毛体。将获得的每种前鞭毛体中的白蛉肠道转移至NNN培养基的试管中以分离寄生虫。结果，从三种白蛉中获得了六种利什曼原虫分离株。从三只沙鼠的耳廓中建立了三种利什曼原虫寄生虫分离株。在对自然感染的大耳进行组织病理学检查时，在耳廓中观察到无鞭毛体，但在脾脏和肝脏中未观察到无鞭毛体。未获得明显的病理变化。 8只近交BALB/c小鼠和8只蒙古沙鼠皮下接种8株利什曼原虫KMA-2、KMA-4和KMA-7（分离自大沙鼠）和KMP-1、KMP-2和KMP-3、KMP-4和KMP-6（分离自白蛉），并进行病理和组织学检查。除 KMA-4 外，BALB/c 小鼠对所有分离株均表现出耐药性。相比之下，在蒙古沙鼠的实验中，除KMP-1和KMP-6之外的利什曼原虫分离株均显示出高传染性。成功地从感染每种分离株的蒙古沙鼠的鼻子中重新分离出前鞭毛体。对建立的四个分离株的迷你外显子基因进行了分析。通过与参考菌株L.major、L.gerbilli和L.turanica的序列比对，从大沙鼠中分离出的利什曼原虫寄生虫被鉴定为L.gerbilli（KMA-4）和L.turanica（KMA-2），从白蛉中分离出的寄生虫被鉴定为L.gerbilli（KMP-3）和L.turanica （KMP-2）。较少的

项目成果

Yoshitsugu Matsumoto, Toshimitsu Naya: "Differentiation of Th cells in Leishmaniasis"Igakunoayumi.. 183(4). 257-261 (1997)

Yoshitsugu Matsumoto、Toshimitsu Naya：“利什曼病中 Th 细胞的分化”Igakunoayumi.. 183(4)。

L.R.Guan: "Leishmaniasis in the sest China" Proceeding of XIV International Congress for Tropical Medicine and Malaria. 361 (1996)

L.R.Guan：“中国东部的利什曼病”第十四届国际热带医学和疟疾大会论文集。

松本芳嗣: "リーシュマニア症におけるサイトカインと病態"獣医畜産新報 JVM. 50(2). 165-170 (1997)

Yoshitsugu Matsumoto：“利什曼病的细胞因子和病理学”兽医和牲畜杂志 JVM 50(2) (1997)。

松本 芳嗣: "リーシュマニア症におけるサイトカインと病態" 獣医畜産新報 JVM. 50 (2). 165-170 (1997)

Yoshitsugu Matsumoto：“利什曼病的细胞因子和病理学”兽医和牲畜杂志 JVM 50 (2) (1997)。

Kawazu S. Chang KP. et al.: "Stage-independent splicing of transcripts two heterogeneous neighboring genes in Leishmania amazonensis"Gene.. 196(1-2). 49-59 (1997)

河津 S. Chang KP。