Studies on atypical cutaneous leishmaniasis in Sri Lanka

虽然利什曼病在斯里兰卡并不流行，但2003年通过NNN培养和/或吉姆萨染色涂片证实了皮肤利什曼病病例。皮肤利什曼病患者皮损一般数量少，小，水疱状，无溃疡，皮损均匀，病程1.5 ~ 36个月。为了鉴定致病种，在真核生物中高度保守的肌动蛋白编码基因的一部分、利什曼原虫属中特有的小外显子基因和对婴儿利什曼原虫有用的半胱氨酸蛋白酶B基因的核苷酸序列。使用分离的寄生虫分析Donovani辨别。有趣的是，比较斯里兰卡分离株的核苷酸序列与利什曼原虫属的参考菌株。与杜氏乳杆菌s.1.同源性最高，内脏利什曼病的主要致病种类。黑热病检测试纸条用于内脏利什曼病的抗体检测 关于我们 对杜氏利什曼原虫引起的内脏利什曼病具有高度敏感性。所有患者均为阴性。结果证实，斯里兰卡流行的嗜皮寄生虫属于L。Donovani s.1.此外，还调查了斯里兰卡的白蛉动物群。尽管在任何特定的地方病流行点，查明媒介白蛉对规划防治这一疾病十分重要，但斯里兰卡尚未做到这一点。共采集白蛉584只。根据形态特征对白蛉进行鉴定。银足银汉鱼分布广泛，而斯坦氏银汉鱼仅在Golle区采集到。由于银足拟步行虫是L.在斯里兰卡，银足类黑热病可能是人类皮肤利什曼病最有可能的候选媒介。本研究首次报道了由杜氏利什曼原虫1型引起的皮肤利什曼病地方病。少

Although leishmaniasis is not considered to be epidemic in Sri Lanka, cutaneous leishmaniasis cases were confirmed by NNN culture and/or Giemsa-stained smears in 2003. The skin lesions of these cutaneous leishmaniasis patients were generally a few in number, and were small, blister in appearance, non-ulcerative, even lesions lasted 1.5 to 36 months. To identify pathogenic species, the nucleotide sequences of a part of the actin-encoding gene which is highly conserved among eukaryotes, the mini-exon gene which is unique in the genus Leishmania, and cysteine protease B gene which is useful for L.infantumlL. donovani discrimination were analyzed using isolated parasites. Interestingly, the comparison of the nucleotide sequences of Sri Lanka isolates with those of reference strains of Leishmania spp. showed the highest homology with that of L.donovani s.1., a principal pathogenic species of visceral leishmaniasis. Kalazar detect dipstick for visceral leishmaniasis, which is antibody detect … More ion assay to rK39 antigen and highly sensitive for visceral leishmaniasis caused by L.donovani s.1. was done and all patients were negative. The results confirm that the prevalent dermotropic parasite in Sri Lanka belongs to the L. donovani s.1. In addition, the sand fly fauna of Sri Lanka was investigated. Although, the identification of vector sand flies is important for the planning of control against this disease in any given endemic site, it has not been achieved yet in Sri Lanka. Five hundred and eighty four sand flies were collected in total. Identification of sand flies was performed based on morphological features. P.argentipes were widely distributed in the island whereas P.stantoni were collected only in Golle district. Since P.argentipes is know to be the vector of L. donovani causing visceral leishmaniasis in India, P.argentipes might be the most possible candidate vector of human cutaneous leishmaniasis in Sri Lanka. The present study described at the first time an endemic of cutaneous leishmaniasis caused by L.donovani s.1.. Less