EPIDEMIOLOGICAL STUDY ON LYME DISEASE IN THE FAR EASTERN COUNTRY

远东国家莱姆病流行病学研究

• 批准号: 08041181
• 负责人: MASUZAWA Toshiyuki
• 金额: $ 7.1万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08041181/
• 关键词: LYME DISEASE; BORRELIA; IXODES; BORRELIA BURGDORFERI; BORRELIA GARINII; BORRELIA AFZELII; SYSTEMATIC CLASSIFICATION; FAR EAST; Borrelia burgderteri; ライムボレリア症

We made Lyme disease borrelia survey of vector ticks and resevoir animals in Northern part of China and the Yangtze River valley, and Korea. Borrelia isolates from these sources and from far eastern Russia we got previously were analyzed by 5S-23S rRNA intergenic spacer and flagellin gene PCR-RFLP analysis and sequence analysis.Isolates from Ixodes persulcatus collected Northern China and far eastern Russia were identified as B.afzelii or B.garinii. B.garinii generate RFLP pattern C which is commonly found in Japan but not in Europe were dominant species in these area. Furthermore, isolates from I.nipponensis collected in central Korea were identified as B.garinii of far eastern type or B.afzelii. Isolates from I.nipponensis tentatively described as B.afzelii-related group obtained from Korean researcher were characterized on intergenic spacer and 16SrRNA sequences. These isolates showed relatively higher similarity values to B.valaisiana isolated from I.ricinus in Europe and strain Am501 from I.columnae in Japan. Isolates from Apodemus spp.and Rattus spp.captured in the Yangtze River valley were identified as B.afzelii-related group and new genotype, respectively. Also, one isolate from I.ovatus in the area showed the same feature to those from the Rattus spp. Further study to described new species is undertaken.By the phylogenetic analysis based on the sequence of outer surface protein A,B,and C,genes, transmission of B.garinii and B.afzelii between Japan and Europe via far eastern country, such as far east Russia, northern China, and Korea was suggested.

我们对我国北方、长江流域和韩国的媒介蜱和水库动物进行了莱姆病疏螺旋体调查。通过5S-23 SrRNA基因间隔区和鞭毛蛋白基因PCR-RFLP分析及序列分析，将采集自中国北方和俄罗斯远东地区的全沟硬蜱分离株鉴定为B.afzelii或B. garinii。B.garinii产生的RFLP模式C在日本很常见，但在欧洲没有，是这些地区的优势种。此外，从韩国中部采集的日本I.nipponensis分离株被鉴定为远东型B.garinii或B. afzelii。从韩国获得的日本硬蜱分离物暂时被描述为阿氏B. afzelii相关组，其特征在于基因间隔区和16 SrRNA序列。这些分离物与欧洲的野蓖麻B.valaisiana分离物和日本的柱状野豌豆Am 501菌株具有较高的相似性。从长江流域捕获的黑线姬鼠属和家鼠属分离株分别鉴定为阿氏布氏杆菌相关群和新基因型。B. afzelii。另外，从该地区卵形硬蜱分离到的一个分离物也表现出与家鼠分离物相同的特征。对新发现的种进行了进一步的研究，根据外表面蛋白A、B和C基因序列的系统发育分析，提出了B.garinii和B.afzelii在日本和欧洲之间通过远东国家，如俄罗斯远东、中国北方和韩国传播的可能性。

项目成果

Toshiyuki Masuzawa et al.: "Genetic diversity of Borrelia burgdorferi sensu lato isolated in far eastern Russia." Microbiol.Immunol.41. 595-600 (1997)

Toshiyuki Masuzawa 等人：“俄罗斯远东地区分离出的伯氏疏螺旋体的遗传多样性。”

Toshiyuki Masuzawa, Tetsuro komikado, Atue Iwaki, Hiroyuki Suzuki, Kazuhide Kaneda, and Yasutake Yanagihara.: "Characterization of Borrelia sp.Iiolated from Ixodes tanuki, I.turdus, and I.columnae in Japan by restriction fragment length polymorphism of rr

Toshiyuki Masuzawa、Tetsuro komikado、Atue Iwaki、Hiroyuki Suzuki、Kazuhide Kaneda 和 Yasutake Yanagihara。：“通过 rr 的限制性片段长度多态性表征来自日本狸蜱、I.turdus 和 I.columnae 的疏螺旋体 sp.Iiolated”

Jianhui Wang, Toshiyuki Masuzawa, Tetsuro komikado, and Yasutake Yanagihara.: "Consensus sequence on the genes encoding the major outer surface proteins (OspA and OspB) of Borrelia garnii isolate." Microbiol.Immunol.41. 83-91 (1997)

Kenhui Wang、Toshiyuki Masuzawa、Tetsuro komikado 和 Yasutake Yanagihara：“编码卡尼疏螺旋体分离物主要外表面蛋白（OspA 和 OspB）的基因的共有序列。”

Kazuhide Kaneda, Toshiyuki Masuzawa, Kitsuzo Yasugami, Takashi Suzuki, Yasuo Suzuki, and Yasutake Yanagihara.: "Glycosphingolipid-binding protein of Borrelia burgdorferi sensu lato." Infect.Immun.65. 3180-3185 (1997)

Kazuhide Kaneda、Toshiyuki Masuzawa、Kitsuzo Yasugami、Takashi Suzuki、Yasuo Suzuki 和 Yasutake Yanagihara：“伯氏疏螺旋体的糖鞘脂结合蛋白”。

FEMS Immunol.Med.Microbiol.18. 249-261 (1997)

FEMS 免疫医学微生物学 18。